Sex differences of subpopulation composition of lymphocytes in the peripheral blood in experimental acute and chronic ulcerative colitis
https://doi.org/10.15789/1563-0625-SDO-1661
Abstract
We studied sex differences lymphocytes subpopulations of peripheral blood in adult C57Bl/6 mice during acute and chronic colitis, induced with 1% DSS. We measured subpopulations of lymphocytes with flow cytometry. We showed that in the control group the female mice had statistically significantly higher values of the relative number of regulatory and cytotoxic T lymphocytes comparing to the males. During acute colitis the females showed an increase in the relative number of Thelpers and a decrease of cytotoxic Tlymphocytes, which reflects the activation of immune response. The males had a decrease in the absolute number of leukocytes, lymphocytes and cytotoxic and regulatory T lymphocytes, probably because of an increase in migration of these cells to the inflammation locus and local lymph nodes. In chronic colitis the females had a decrease in the absolute number of leukocytes, lymphocytes, T helpers, cytotoxic T lymphocytes and B lymphocytes when comparing with acute colitis. During chronic colitis the males had a decrease in the absolute number of T helpers and B lymphocytes but an increase of regulatory T cells in comparison with the control group; in comparison with acute colitis the males with chronic colitis had higher relative and absolute number of regulatory T cells. The increase of T regulatory lymphocytes is due to an increase in their proliferation rate in the thymus and increase of their migration to the inflammatory locus – the colon. Future clinical studies may be based on these results, which show that the treatment of colitis, especially with immunotropic agents, must take sex differences into account.
About the Authors
O. V. Makarova
Research Institute of Human Morphology
Russian Federation
Russian Federation
Makarova O.V., PhD, MD (Medicine), Professor, Main Research Associate, Head, Laboratory of Immunomorphology of Inflammation
117418, Moscow, Tsyurupa str., 3.
E. A. Postovalova
Research Institute of Human Morphology
Russian Federation
Russian Federation
Postovalova E.A., Research Associate, Laboratory of Immunomorphology of Inflammation
117418, Moscow, Tsyurupa str., 3.
Yu. Gao
M. Lomonosov Moscow State University
Russian Federation
Russian Federation
Gao Yuan, Postgraduate Student, Department of Cell Biology and Histology, Faculty of Biology
Moscow
M. T. Dobrynina
M. Lomonosov Moscow State University
Russian Federation
Russian Federation
Dobrynina M.T., PhD (Biology), Senior Lecturer, Department of Cell Biology and Histology, Faculty of Biology
Moscow
References
1. Kravchenko P.N., Oleinik E.K. The system of regulatory T cells and autoimmunity. Trudy Karelskogo nauchnogo tsentra RAN = Transactions of Karelian Research Centre of the Russian Academy of Sciences, 2013, no. 3, pp. 18-30. (In Russ.)
2. Angele M.K., Frantz M.C., Chaudry I.H. Gender and sex hormones influence the response to trauma and sepsis: potential therapeutic approahes. Clinics, 2006, Vol. 61, no. 5, pp. 479-488.
3. Bouman A., Heineman M.J., Faas M.M. Sex hormones and the immune response in humans. Hum. Reprod. Update, 2005, Vol. 11, no. 4, pp. 411-423.
4. Fish E.N. The X-files in immunity: sex-based differences predispose immune responses. Nat. Rev. Immunol., 2008, Vol. 8, no. 9, pp. 737-744.
5. Frisullo G., Nociti V., Iorio R., Patanella A.K., Caggiula M., Marti A., Sancricca C., Angelucci F., Mirabella M., Tonali P.A., Batocchi A.P. Regulatory T cells fail to suppress CD4+T-bet+ T cells in relapsing multiple sclerosis patients. Immunology, 2009, no. 127, pp. 418-428.
6. Giefing-Kröll C., Berger P., Lepperdinger G., Grubeck-Loebenstein B. How sex and age affect immune responses, susceptibility to infections, and response to vaccination. Aging Cell, 2015, Vol. 14, no. 3, pp. 309-321.
7. Glickman R.M. Inflammatory bowel disease: ulcerative colitis and Crohn’s disease. In: Wilson J.D., Braunwald E., Isselbacher K.J. Harrison’s principles of internal medicine. New York, NY: McGraw-Hill, Inc., 1991, pp. 1268-1281.
8. Kim I.K., Park K.J., Kang G.H., Im J.P., Kim S.G., Jung H.C., Song I.S., Kim J.S. Risk factors for complications after total colectomy in ulcerative colitis. Turk J. Gastroenterol., 2012, Vol. 23, no. 5, pp. 515-522.
9. Kissick H.T., Sanda M.G., Dunn L.K., Pellegrini K.L., On S.T., Noel J.K., Arredouani M.S. Androgens alter T-cell immunity by inhibiting T-helper 1 differentiation. Proc. Natl. Acad. Sci. USA, 2014, Vol. 111, no. 27, pp. 9887-9892.
10. Knöferl M.W., Angele M.K., Schwacha M.G., Bland K., Chaudry I.H. Preservation of splenic immune functions by female sex hormones after trauma-hemorrhage. Crit. Care. Med., 2002, Vol. 30, pp. 888-893.
11. Kryczek I., Wu K., Zhao E., Wei S., Vatan L., Szeliga W., Huang E., Greenson J., Chang A., Roliński J., Radwan P., Fang J., Wang G., Zou W. IL-17+ regulatory T cells in the microenvironments of chronic inflammation and cancer. J. Immunol., 2011, no. 186, pp. 4388-4395.
12. Latham K.A., Zamora A., Drought H., Subramanian S., Matejuk A., Offner H., Rosloniec E.F. Estradiol treatment redirects the isotype of the autoandibody response and prevents the development of autoimmune arthritis. J. Immunol., 2003, Vol. 171, pp. 5820-5827.
13. Levings M.K., Bacchetta R., Schulz U., Roncarolo M.G. The role of IL-10 and TGF-beta in the differentiation and effector function of T regulatory cells. Int. Arch. Allergy Immunol., 2002, no. 129, pp. 263-276.
14. Maul J., Loddenkemper C., Mundt P., Berg E. Giese T., Stallmach A., Zeitz M., Duchmann R. Peripheral and intestinal regulatory CD4+CD25(high) T cells in inflammatory bowel disease. Gastroenterology, 2005, no. 128, pp. 1868-1878.
15. Mullen A.C., High F.A., Hutchins A.S., Lee H.W., Villarino A.V., Livingston D.M., Kung A.L., Cereb N., Yao T.P., Yang S.Y., Reiner S.L. Role of T-bet in commitment of Th1 cells before IL-12-dependent selection. Science, 2001, Vol. 292, pp. 1907-1910.
16. Pedros C., Duguet F., Saoudi A., Chabod M. Disrupted regulatory T cell homeostasis in inflammatory bowel diseases. World J. Gastroenterol., 2016, Vol. 22, no. 3, pp. 974-995.
17. Powrie F. Immune regulation in the intestine: a balancing act between effector and regulatory T cell responses. Ann. N.Y. Acad. Sci., 2004, no. 1029, pp. 132-141.
18. Roberts C.W., Walker W., Alexander J. Sex-associated hormones and immunity to protozoan parasites. Rev. Clin. Microbiol., 2010, Vol. 14, pp. 476-488.
19. Suen J.L., Li H.T., Jong Y.J., Chiang B.L., Yen J.H. Altered homeostasis of CD4(+) FoxP3(+) regulatory T-cell subpopulations in systemic lupus erythematosus. Immunology, 2009, no. 127, pp. 196-205.
20. Walecki M., Eisel F., Klug J., Baal N., Paradowska-Dogan A., Wahle E., Hackstein H., Meinhardt A., Fijak M. Androgen receptor modulates Foxp3 expression in CD4+CD25+Foxp3+ regulatory T-cells. Mol. Biol. Cell, 2015, Vol. 26, no. 15, pp. 2845-2857.
21. Wang Y., Liu X.P., Zhao Z.B., Chen J.H., Yu C.G. Expression of CD4+ forkhead box P3 (FOXP3)+ regulatory T cells in inflammatory bowel disease. J. Dig. Dis., 2011, no. 12, pp. 286-294.
22. Yu T.Q., Saruta M., Avanesyan A., Fleshner R.F., Banham H.A., Papadakis A.K. Expression and functional characterization of FOXP3CD4 regulatory T cells in ulcerative colitis. Inflamm. Bowel Dis., 2007, Vol. 13, no. 2, pp. 191-199.
Supplementary files
|
1. Метаданные | |
| Subject | ||
| Type | Исследовательские инструменты | |
Download
(14KB)
|
Indexing metadata ▾ | |
|
|
2. Титульный лист | |
| Subject | ||
| Type | Исследовательские инструменты | |
Download
(14KB)
|
Indexing metadata ▾ | |
|
|
3. Резюме | |
| Subject | ||
| Type | Исследовательские инструменты | |
Download
(14KB)
|
Indexing metadata ▾ | |
|
|
4. Подписи авторов | |
| Subject | ||
| Type | Исследовательские инструменты | |
View
(2MB)
|
Indexing metadata ▾ | |
|
|
5. Рисунок 1 | |
| Subject | ||
| Type | Исследовательские инструменты | |
Download
(23KB)
|
Indexing metadata ▾ | |
|
|
6. Подпись к рисунку 1 | |
| Subject | ||
| Type | Исследовательские инструменты | |
Download
(12KB)
|
Indexing metadata ▾ | |
|
|
7. Таблица 1 | |
| Subject | ||
| Type | Исследовательские инструменты | |
Download
(14KB)
|
Indexing metadata ▾ | |
|
|
8. Таблица 2 | |
| Subject | ||
| Type | Исследовательские инструменты | |
Download
(16KB)
|
Indexing metadata ▾ | |
|
|
9. Таблица 3 | |
| Subject | ||
| Type | Исследовательские инструменты | |
Download
(16KB)
|
Indexing metadata ▾ | |
|
|
10. Литература | |
| Subject | ||
| Type | Исследовательские инструменты | |
Download
(19KB)
|
Indexing metadata ▾ | |
|
|
11. Таблица 1 | |
| Subject | ||
| Type | Other | |
Download
(16KB)
|
Indexing metadata ▾ | |
|
|
12. Таблица 2 | |
| Subject | ||
| Type | Other | |
Download
(20KB)
|
Indexing metadata ▾ | |
|
|
13. Таблица 3 | |
| Subject | ||
| Type | Other | |
Download
(20KB)
|
Indexing metadata ▾ | |
|
|
14. Литература | |
| Subject | ||
| Type | Other | |
Download
(21KB)
|
Indexing metadata ▾ | |
|
|
15. Таблица 1 | |
| Subject | ||
| Type | Other | |
Download
(14KB)
|
Indexing metadata ▾ | |
|
|
16. Таблица 2 | |
| Subject | ||
| Type | Other | |
Download
(16KB)
|
Indexing metadata ▾ | |
|
|
17. Таблица 3 | |
| Subject | ||
| Type | Other | |
Download
(16KB)
|
Indexing metadata ▾ | |
|
|
18. Таблица 1 | |
| Subject | ||
| Type | Other | |
Download
(14KB)
|
Indexing metadata ▾ | |
|
|
19. Таблица 2 | |
| Subject | ||
| Type | Other | |
Download
(16KB)
|
Indexing metadata ▾ | |
|
|
20. Таблица 3 | |
| Subject | ||
| Type | Other | |
Download
(16KB)
|
Indexing metadata ▾ | |
Review
For citations:
Makarova O.V., Postovalova E.A., Gao Yu., Dobrynina M.T. Sex differences of subpopulation composition of lymphocytes in the peripheral blood in experimental acute and chronic ulcerative colitis. Medical Immunology (Russia). 2020;22(1):157-164. (In Russ.) https://doi.org/10.15789/1563-0625-SDO-1661
Views:
984
Email this article 