Grculation of microparticles of lymphocytic origin at critical ischemia of the lower extremities

Great scientific interest to problems of obliterating atherosclerosis of vessels of the lower extremities is caused by increase in incidence of this pathology and multidisciplinary approach in diagnostics and treatment the research objective: to study interrelations between quantity of the circulating microparticles of lymphocytic origin and development of critical ischemia of the lower extremities. The executed research belonged to cohort prospective researches. Two studied groups were created: 1^st group included 75 patients with ischemia IIB degrees on Pokrovsky—Fontaine's classification. The 2nd group included 75 patients with clinical manifestations of critical ischemia. Also, the group of control of 75 almost healthy faces which do not have symptoms of obliterating diseases of vessels of the lower extremities was created. For objectification of assessment of degree of ischemia measured anklebone’s arterial blood pressure, the anklebone-humeral index, toe’s arterial blood pressure, Transcutaneous tension of oxygen in foot fingers. For blood-groove assessment in arteries of the lower extremities used ultrasonic doppler sonography of arteries of the lower extremities and the KT-angiography. The research was conducted at arrival of the patient in a hospital prior to therapy. Lymphocytes and the circulating microparticles defined according to the standard protocol. At almost healthy faces the number of the circulating microparticles of lymphocytic origin made of control group 385 (260479) on 100 lymphocytes. In 1^st group this indicator was twice higher and made 728 (654-836) (p₁ = 0.002). In the 2^nd group it was 5 times higher, than in group of control and authentically differed from an indicator of 1^st group (1570 (1124-2120) (p₁< 0.001, p₂ = 0.002) the Correlation coefficient between number of the circulating microparticles and anklebone’s arterial blood pressure r_S = -0.82 (p = 0.003), Correlation between the anklebone-humeral index and number of microparticles was equal to r_S = -0.92 (p < 0.001). Toe’s arterial blood pressure had average force negative correlation with number of microparticles. The transcutaneous tension of oxygen and number of microparticles had a correlation coefficient equal rS = -0.89 (p = 0.002). The correlation coefficient between a gleam of the general femoral artery and number of microparticles of lymphocytic origin made r_S = -0.44 (p = 0.016). A correlation coefficient between a gleam of a superficial femoral artery and number of microparticles of r_S = -0.64 (p = 0.002). Negative correlations of number of microparticles with the kept gleam of a popliteal artery — r_S = -0.79 (p < 0.001), and a back tibial artery — r_S = -0.86 were high, (p < 0.001). Very high negative correlation coefficient was between the kept gleam of a lobby tibial and number of microparticles of lymphocytic origin of r_S = -0.91 (p = 0.003). The revealed high and very high correlation bonds between number of the circulating microparticles of lymphocytic origin and indicators of a condition of an arterial blood-groove of the lower extremities, and the kept gleam of arteries of the lower extremities allow to speak about the prospects of use of microparticles as pathogenetic marker of critical ischemia of the lower extremities.

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