Dynamics of changes in B1 and B2 lymphocyte populations in articular form of seronegative rheumatoid arthritis
https://doi.org/10.15789/1563-0625-2019-6-1073-1080
Abstract
Immunopathogenesis of rheumatoid arthritis (RA) is characterized by polyclonal activation of B lymphocytes, changes in their subpopulation composition, impaired peripheral tolerance, increased activation and differentiation of B cells, as well as deficient negative selection. Peripheral blood phenotypes of B1 and B2 lymphocytes, their correlation and response patterns in seronegative variant of articular RA type A decrease in B1 lymphocytes along with increased degree of clinical activity and duration of RA has been revealed. B2 lymphocytes remained the predominant subpopulation in peripheral blood. The studied parameters of B2 lymphocytes in the patients with disease duration of up to 5 years and more were higher than the data obtained in a control group. However, no significant changes in their dynamics were revealed upon comparative analysis. With increasing degree of clinical activity, the decreased activation potential of B2 lymphocytes was revealed, along with reduction of costimulatory signal transmission via the CD40/CD40L complex. Hence, detection of B1 and B2 lymphocytes in various types of RA may be used as diagnostic and prognostic markers.
About the Authors
L. P. Sizyakina
Rostov State Medical University
Russian Federation
Competing Interests: not
Russian Federation
Sizyakina Lyudmila Petrovna - PhD, MD (Medicine), Professor, Head, Department of Clinical Immunology and Allergology, Director, Research Institute of Clinical Immunology.
Rostov-on-Don
Competing Interests: not
M. V. Kharitonova
Rostov State Medical University
Russian Federation
Competing Interests: not
Russian Federation
Kharitonova Maria Vladimirovna - Head, Laboratory of Clinical Immunology and Allergology.
344022, Rostov-on-Don, Nakhichevansky lane, 29., Phone: 7 (863) 263-44-41
Competing Interests: not
References
1. Baydun L.A., Zurochka A.V., Totolian Areg A., Khaydukov S.V. Standardized technology “Peripheral blood lymphocyte subpopulations by using of flow cytometry-analizators” (project). Meditsinskaya immunologiya = Medical Immunology (Russia), 2012, Vol. 14, no. 3, pp. 255-268. (In Russ.) doi: 10.15789/1563-0625-2012-3-255-268.
2. Budkova A.I., Lapin S.V., Serebryakova M.K., Kudryavtsev I.V., Trishina I.N., Maslyansky A.L., Totolyan Areg A. Subpopulation composition of peripheral blood B cells in patients with systemic lupus erythematosus. Meditsinskaya immunologiya = Medical Immunology (Russia), 2017, Vol. 19, no. 2, pp. 175-184. (In Russ.) doi: 10.15789/1563-0625-2017-2-175-184.
3. Dyakov I.N., Sidorova E.V. Subpopulations of B lymphocytes and the effect of the microenvironment on their functional activity. Pulmonologiya = Pulmonology, 2010, no. 5, pp. 116-123. (In Russ.)
4. Karateev D.E., Olyunin Yu.A., Luchikhina E.L. New classification criteria for rheumatoid arthritis ACR/EULAR 2010 a step forward to early diagnosis. Naucho-prakticheskaya revmatologiya = Rheumatology Science and Practice, 2011, Vol. 49, no. 1, pp. 10-15. (In Russ.)
5. Kozlov V.A. Suppressor cells are the basis of the immunopathogenesis of autoimmune diseases. Meditsinskaya immunologiya = Medical Immunology (Russia), 2016, Vol. 18, no. 1, pp. 7-14. (In Russ.) doi: 10.15789/1563-0625-2016-1-7-14.
6. Kudryavtsev I.V, Ilves A.G., Krobinets I. I., Mineev K.K., Serebryakova M.K., Petrov A.M., Stolyarov I.D. Changes in the subpopulation composition ofperipheral blood B lymphocytes in multiple sclerosis. Rossiyskiy immunologicheskiyzhurnal = Russian Journal of Immunology, 2017, Vol. 11 (20), no. 2, pp. 150-154. (In Russ.)
7. Lazareva N.M., Lapin S.V., Mazing A.V., Bulgakova T.V., Ilivanova E.P., Maslyansky A.L., Totolian A.A. Optimization of serological diagnostic methods of connective tissue diseases. Klinicheskaya laboratornaya diagnostika = Clinical Laboratory Diagnostics, 2011, no. 12, pp. 12-17. (In Russ.)
8. Nasonov E.L. Inhibition of immune control points and autoimmunity: rheumatological problems. Naucho-prakticheskaya revmatologiya = Rheumatology Science and Practice, 2018, Vol. 56, no. 1, pp. 5-9. (In Russ.)
9. Nasonov E.L. Problems of immunopathology of rheumatoid arthritis: the evolution of the disease. Naucho-prakticheskaya revmatologiya = Rheumatology Science and Practice, 2017, Vol. 55, no. 3, pp. 277-294. (In Russ.)
10. Sidorova E.V. Long-lived B cells. Uspekhi sovremennoy biologii = Successes of Modern Biology, 2013, Vol. 133, no. 4, pp. 333-348. (In Russ.)
11. Sizyakina L.P., Kharitonova M.V. Characteristics of B2 lymphocytes in patients with seropositive rheumatoid arthritis of the articular form. Immunologiya = Immunology, 2017, no. 4, pp. 226-228. (In Russ.)
12. Sizyakina L.P., Kharitonova M.V. Characteristics of B2 lymphocytes in patients with seronegative rheumatoid arthritis of the articular form. Immunologiya = Immunology, 2018, Vol. 39, no. 2-3, pp. 134-136. (In Russ.)
13. Sizyakina L.P., Kharitonova M.V. Features of B lymphocyte phenotypes depending on the duration of the seropositive variant of the articular form of rheumatoid arthritis. Rossiyskiy allergologicheskiy zhurnal = Russian Allergology Journal, 2019, Vol. 16, no. 1, Pt 2, pp. 141-143. (In Russ.)
14. Suponitskaya E.V., Aleksandrova E.N., Aleksankin A.P., Nasonov E.L. The effect of therapy with genetically engineered biological preparations on subpopulations of B-lymphocytes in rheumatic diseases: new data. Naucho-prakticheskaya revmatologiya = Rheumatology Science and Practice, 2015, Vol. 53, no. 1, pp. 78-83. (In Russ.)
15. Suponitskaya E.V., Aleksankin A.P., Aleksandrova E.N., Avdeeva A.S., Panafidina T.A., Verizhnikova Zh.G., Nasonov E.L. Determination of subpopulations of peripheral blood B lymphocytes using flow cytofluorometry in healthy individuals and patients with rheumatic diseases. Klinicheskaya laboratornaya diagnostika = Clinical Laboratory Diagnostics, 2015, Vol. 60, no. 6, pp. 30-33. (In Russ.)
16. Suprun E.N. Humoral immune response. Allergologiya i immunologiya vpediatrii = Allergology and Immunology in Pediatrics, 2014, no. 4 (39), pp. 44-48. (In Russ.)
17. Ярилин А.А. Иммунология. М.: ГЭОТАР-Медиа, 2010. 752 с. [Yarilin A.A. Immunology]. Moscow: GEOTAR-Media, 2010. 752 p.
18. Adlowitz D.G., Barnard J., Biear J.N., Cistrone C., Owen T., Wang W., Palanichamy A., Ezealah E., Campbell D., Wei C., Looney R.J., Sanz I., Anolik J.H. Expansion of activated peripheral blood memory B cells in rheumatoid arthritis, impact of B cell depletion therapy, and biomarkers of response. PLoS ONE, 2015, Vol. 10, no. 6, e0128269. doi: 10.1371/journal.pone.0128269.
19. Bugatti S., Bogliolo L., Montecucco C., Manzo А. B cell autoimmunity and bone damage in rheumatoid arthritis. Reumatismo, 2016. Vol. 68, no. 3, pp. 117-125.
20. Bugatti S., Vitolo B., Caporali R., Montecucco C., Manzo A. B cells in rheumatoid arthritis: from pathogenic players to disease biomarkers. BioMed. Res. Int., 2014, Vol. 2014, 681678. doi: 10.1155/2014/681678.
21. Vale A.M., Kearney J.F., Nobrega A., Schroeder H.W Molecular biology of B cells. 3rd ed. 2015, pp. 99-119.
Supplementary files
|
1. Неозаглавлен | |
| Subject | ||
| Type | Other | |
Download
(16KB)
|
Indexing metadata ▾ | |
|
|
2. Динамика изменений В1 и В2-лимфоцитов при серонегативном ревматоидном артрите суставной формы | |
| Subject | серонегативный ревматоидный артрит, В1 и В2-лимфоциты, ко-стимулирующие молекулы, проточная цитометрия | |
| Type | Результаты исследования | |
Download
(16KB)
|
Indexing metadata ▾ | |
|
3. Динамика изменений В1 и В2-лимфоцитов при серонегативном ревматоидном артрите суставной формы | |
| Subject | серонегативный ревматоидный артрит, В1 и В2-лимфоциты, ко-стимулирующие молекулы, проточная цитометрия | |
| Type | Результаты исследования | |
Download
(B)
|
Indexing metadata ▾ | |
|
|
4. Таблица1. Показатели В-лимфоцитов при серонегативном варианте суставной формы РА | |
| Subject | ||
| Type | Результаты исследования | |
Download
(15KB)
|
Indexing metadata ▾ | |
|
|
5. Таблица 2. Показатели В-лимфоцитов серонегативного варианта суставной формы РА в зависимости от длительности течения | |
| Subject | ||
| Type | Результаты исследования | |
Download
(16KB)
|
Indexing metadata ▾ | |
|
|
6. TABLE 3. PARAMETERS OF B-LYMPHOCYTES WITH DIFFERENT DEGREES OF ACTIVITY OF THE SERONEGATIVE VARIANT OF ARTICULAR FORM RA | |
| Subject | ||
| Type | Результаты исследования | |
Download
(16KB)
|
Indexing metadata ▾ | |
|
|
7. Литература | |
| Subject | ||
| Type | Other | |
Download
(33KB)
|
Indexing metadata ▾ | |
Review
For citations:
Sizyakina L.P., Kharitonova M.V. Dynamics of changes in B1 and B2 lymphocyte populations in articular form of seronegative rheumatoid arthritis. Medical Immunology (Russia). 2019;21(6):1073-1080. (In Russ.) https://doi.org/10.15789/1563-0625-2019-6-1073-1080
Views:
1980
Email this article 