EXPRESSION OF AID-SPECIFIC mRNA IN PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA
https://doi.org/10.15789/1563-0625-2015-1-71-74
- Р Р‡.МессенРТвЂВВВВВВВВжер
- РћРТвЂВВВВВВВВнокласснРСвЂВВВВВВВВРєРСвЂВВВВВВВВ
- LiveJournal
- Telegram
- ВКонтакте
- РЎРєРѕРїРСвЂВВВВВВВВровать ссылку
Full Text:
Abstract
The aim of this study was to evaluate a possible role of AID (AICDA – activation-induced (cytidine) deaminase) in the bronchial asthma (BA) pathogenesis. Materials and methods. We have examined twelve healthy control persons, forty-two patients with allergic bronchial asthma (ABA) and twenty-seven patients with non-allergic bronchial asthma (NABA). The AID mRNA expression was evaluated by means of RT-PCR. Results: AID mRNA was more significantly expressed in BA, than in healthy controls. Meanwhile, no significant differences were revealed between ABA and NABA groups. Correlation analysis of AID mRNA expression levels in peripheral blood lymphocytes has shown that CHε mRNA and total serum IgE revealed significant negative correlation, in the NABA group only. The levels of AID mRNA expression in peripheral blood lymphocytes exhibited positive and significant correlations with clinical characteristics, reflecting severity and stage of the disease, in BA patients. Moreover, a significant positive correlation was revealed with eosinophil contents in sputum. Conclusion. It was concluded that normal regulation of IgE class switching normally based on feedback regulation, was impaired in ABA but not affected in NABA.
About the Authors
V. N. Mineev
St. Petersburg State I. Pavlov Medical University
198156, Russian Federation, St. Petersburg, Petrodvorets, St. Petersburg pr., 56, apt 15.
Russian Federation
Russian Federation
PhD, MD (Medicine), Professor,
M. Chernorutsky Chair of Hospital Therapy, St. Petersburg State I. Pavlov Medical University, St. Petersburg, Russian Federation
M. A. Nyoma
St. Petersburg State I. Pavlov Medical University
198156, Russian Federation, St. Petersburg, Petrodvorets, St. Petersburg pr., 56, apt 15.
Russian Federation
Russian Federation
PhD (Medicine), Assistant Professor,
M. Chernorutsky Chair of Hospital Therapy, St. Petersburg State I. Pavlov Medical University, St. Petersburg, Russian Federation
L. N. Sorokina
St. Petersburg State I. Pavlov Medical University
198156, Russian Federation, St. Petersburg, Petrodvorets, St. Petersburg pr., 56, apt 15.
Russian Federation
Russian Federation
PhD, MD (Medicine), Professor, M. Chernorutsky Chair of Hospital Therapy, St. Petersburg State I. Pavlov Medical University, St. Petersburg, Russian Federation
References
1. Mineev V.N., Sorokina L.N., Nëma M.A., Eremeeva A.V. Vzaimodeĭstvie transkriptsionnykh faktorov PAX-5 i STAT6 v patogeneze allergicheskoĭ bronkhial'noĭ astmy // Meditsinskaya immunologiya, 2014. T. 16, No 1. S. 35-42. [ Mineev V.N., Sorokina L.N., Nyoma M.A., Eremeeva A.V. Interaction of transcription factors PAX-5 and STAT6 in pathogenesis of allergic bronchial asthma. Meditsinskaya immunologiya = Medical Immunology (Russia), 2014, Vol. 16. no. 1. pp. 35-42. (In Russ.)]
2. Sorokina L.N., Nëma M.A., Ivanov V.A., Lipkin G.I., Mineev V.N. Rol' transkriptsionnogo faktora PAX-5 v immunologicheskikh protsessakh // Meditsinskaya immunologiya, 2011. T. 13, No 6. S. 569580. [Mineev V.N., Sorokina L.N., Nyoma M.A., Ivanov V.A., Lipkin G.I . Role of PAX-5 transcription factor in immunological processes. Meditsinskaya immunologiya = Medical Immunology (Russia), 2011. Vol. 13, no 6, pp. 569-580. (In Russ.)]
3. Coker H.A., Durham S.R., Gould H.J. Local somatic hypermutation and class switch recombination in the nasal mucosa of allergic rhinitis patients. J. Immunol., 2003, Vol. 171, pp. 5602-5610.
4. Laffleur B., Denis-Lagache N., Péron S., Sirac C., Moreau J., Cogné M. AID-induced remodeling of immunoglobulin genes and B cell fate. Oncotarget, 2014, no. 5. pp. 1118-1131.
5. Takhar P., Corrigan C.J., Smurthwaite L., O’Connor B.J., Durham S.R., Lee T.H., Gould H.J. Class switch recombination to IgE in the bronchial mucosa of atopic and nonatopic patients with asthma. J. Allergy Clin. Immunol., 2007, Vol. 119. pp. 213-218.
6. Takhar P., Smurthwaite L., Coker H.A., Fear D.J., Banfield G.K., Carr V.A., Durham S.R., Gould H.J. Allergen drives class switching to IgE in the nasal mucosa in allergic rhinitis. J. Immunol., 2005. Vol. 174. pp. 5024-5032.
Review
For citations:
Mineev V.N., Nyoma M.A., Sorokina L.N. EXPRESSION OF AID-SPECIFIC mRNA IN PERIPHERAL BLOOD LYMPHOCYTES IN BRONCHIAL ASTHMA. Medical Immunology (Russia). 2015;17(1):71-74. (In Russ.) https://doi.org/10.15789/1563-0625-2015-1-71-74
Views:
1047
ISSN 1563-0625 (Print)
ISSN 2313-741X (Online)
ISSN 2313-741X (Online)
Email this article 