LYMPHOCYTE SUBPOPULATIONS IN GASTRIC AND COLORECTAL CANCER PATIENTS AFTER IMMUNOTHERAPY WITH ACTIVATED LYMPHOCYTES
https://doi.org/10.15789/1563-0625-2014-5-449-456
Abstract
Phenotyping of peripheral blood lymphocytes and immune activation markers of activation (HLADR, CD38, CD69, CD314, CD25) was performed in thirteen patients with disseminated forms of stomach and rectal cancer before cell treatment, and following adoptive immunotherapy with activated lymphocytes. It was
shown that the lymphocytes are well activated and are able to proliferate in vitro. It was revealed that the relative content of Tregs and NK cells is increased in these patients. After the courses of immunotherapy, initially low levels of B-cells (average 5%) remained in all the patients, whereas concentration of Тregs didn’t change. Increased expression of activation markers was revealed for all lymphocyte subsets (CD25, CD314, CD38, HLA-DR) and, especially, for T-lymphocytes (CD3+HLA-DR, CD3+CD38+). Significant decrease of T helpers, activated NK-cells (CD16+CD314+) and CD4+/CD8+ was noted in peripheral blood. A non-significant elevation in mature lymphocytes (CD45RO+) and reduced content of young lymphocytes (CD45RA+) were revealed. Adoptive immunotherapy is safe and well tolerated, being characterized by lack of side effects, and it may be recommended as a complement to conventional radio- and chemotherapy.
About the Authors
E. V. Abakushina
Medical Radiological Research Center, Russian Ministry of Health Care, Obninsk, Russian Federation
Russian Federation
Russian Federation
PhD, Senior Research Associate, Laboratory of Clinical Immunology, Medical Radiological Research Center, Russian
Ministry of Health Care 249036, Russian Federation, Obninsk, Kaluga Region, Koroleva str., 4. Phone: 7 (484) 392-96-04.
Fax: 7 (495) 956-14-40
Yu. V. Marizina
Medical Radiological Research Center, Russian Ministry of Health Care, Obninsk, Russian Federation
Russian Federation
Russian Federation
Laboratory Research Assistant, Science Organization Department, Medical Radiological Research Center, Russian Ministry of Health Care, Obninsk, Russian Federation
G. S. Neprina
Medical Radiological Research Center, Russian Ministry of Health Care, Obninsk, Russian Federation
Russian Federation
Russian Federation
PhD (Medicine), Leading Researcher, Laboratory of Clinical Immunology, Medical Radiological Research Center, Russian Ministry of Health Care, Obninsk, Russian Federation
I. A. Pasova
Medical Radiological Research Center, Russian Ministry of Health Care, Obninsk, Russian Federation
Russian Federation
Russian Federation
Immunologist, Laboratory of Clinical Immunology, Medical Radiological Research Center, Russian Ministry of Health Care, Obninsk, Russian Federation
B. A. Berdov
Medical Radiological Research Center, Russian Ministry of Health Care, Obninsk, Russian Federation
Russian Federation
Russian Federation
PhD, MD (Medicine),Professor, Head, Department of Radiology and Surgery Methods of Therapy, Medical Radiological Research Center, Russian Ministry of Health Care, Obninsk, Russian Federation
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Review
For citations:
Abakushina E.V., Marizina Yu.V., Neprina G.S., Pasova I.A., Berdov B.A. LYMPHOCYTE SUBPOPULATIONS IN GASTRIC AND COLORECTAL CANCER PATIENTS AFTER IMMUNOTHERAPY WITH ACTIVATED LYMPHOCYTES. Medical Immunology (Russia). 2014;16(5):449-456. (In Russ.) https://doi.org/10.15789/1563-0625-2014-5-449-456
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