CXCR3 CHEMOKINE RECEPTOR EXPRESSION ON PERIPHERAL BLOOD B-CELL SUBSETS IN PATIENTS WITH CHRONIC HEPATITIS C
https://doi.org/10.15789/1563-0625-2013-5-471-476
Abstract
Abstract. Present study was aimed to perform a quantitative determination of B lymphocyte subpopulations, i.e., B1, B2, memory B cells and CXCR3-positive B cells in peripheral blood of nineteen patients with chronic hepatitis C (HCV) and thirty-two healthy blood donors. Phenotyping of B cell subsets was performed by means of flow cytometry, using a combination of specific monoclonal antibodies, i.e., CD5-FITC/CD27-PC7/CXCR3-APC/CD19-APC-AF700/CD45-APC-AF750. Total numbers of B-lymphocytes and their subpopulations in peripheral blood of HCV-infected patients did not differ from appropriate values in control group. Meanwhile, expression of CXCR3 chemokine receptor in HCV patients proved to be significantly increased in all subsets of B cells. These results suggest a possible involvement of CXCR3-positive B cells into pathogenesis of chronic HCV infection.
About the Authors
N. A. Arsentieva
St. Petersburg Pasteur Institute of Epidemiology and Microbiology, St. Petersburg
Russian Federation
Russian Federation
Research Associate, Laboratory of Molecular Immunology and Seroepidemiology
197101, Mira str., 14. Phone: 7 (812) 232-31-55
I. V. Kudryavtsev
Research Institute of Experimental Medicine, Russian Academy of Medical Sciences, North-West Branch, St. Petersburg;
Far Eastern Federal University, Vladivostok;
St. Petersburg State University, St. Petersburg
Russian Federation
Russian Federation
PhD (Biology), Senior Researcher,
Department of Immunology; Associate Professor, Department
of Fundamental Medicine; Associate Professor, Department of Cytology and
Histology
D. S. Elezov
St. Petersburg Pasteur Institute of Epidemiology and Microbiology, St. Petersburg
Russian Federation
Russian Federation
Research Associate, Laboratory of Molecular Immunology and Seroepidemiology
A. V. Semenov
St. Petersburg Pasteur Institute of Epidemiology and Microbiology, St. Petersburg
Russian Federation
Russian Federation
PhD (Biology), Chief, Laboratory of HIV Immunology and Epidemiology
E. V. Esaulenko
St. Petersburg State Pediatric Medical University, St. Petersburg
Russian Federation
Russian Federation
PhD, MD (Medicine), Professor, Chief, Department of Adult Infectious Diseases and Epidemiology
V. V. Basina
St. Petersburg State Pediatric Medical University, St. Petersburg
Russian Federation
Russian Federation
Assistant Professor, Department of Adult Infectious Diseases and Epidemiology
Areg A. Totolian
St. Petersburg Pasteur Institute of Epidemiology and Microbiology, St. Petersburg
Russian Federation
Russian Federation
PhD, MD (Medicine), Professor, Corresponding Member, Russian Academy of Medical Sciences, Deputy Director
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Review
For citations:
Arsentieva N.A., Kudryavtsev I.V., Elezov D.S., Semenov A.V., Esaulenko E.V., Basina V.V., Totolian A.A. CXCR3 CHEMOKINE RECEPTOR EXPRESSION ON PERIPHERAL BLOOD B-CELL SUBSETS IN PATIENTS WITH CHRONIC HEPATITIS C. Medical Immunology (Russia). 2013;15(5):471-476. (In Russ.) https://doi.org/10.15789/1563-0625-2013-5-471-476
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