AUTOANTIBODIES TO DNA IN BLOOD SERUM OF PATIENTS WITH HEMORRHAGIC FEVER WITH RENAL SYNDROME
https://doi.org/10.15789/1563-0625-2006-5-6-653-658
Abstract
Abstract. The levels of autoantibodies (AAbs) to double- and single-stranded DNA were studied in blood serum of sixty-one patients with hemorrhagic fever complicated by renal syndrome (HFRS), and twenty-five healthy persons using ELISA technique. In 95% of HFRS patients, the AAb levels to native (double-stranded) DNA were close to their values in healthy group (resp., 0,02-0,26 vs 0,06-0,27 arbitrary units). The levels of AAbs to single-stranded level in serum of patients significantly exceeded the threshold values for healthy persons (resp., 0,37-0,80 vs 0,14-0,57 arbitrary units). The average levels of AAbs to single-stranded DNA in the patients were increased twice, in comparison with normal values. These results demonstrate that hantavirus infection followed by development of HFRS are accompanied by activation of autoimmune processes in the organism, being associated with increased synthesis of AAbs to denatured DNA. AAbs to denatured DNA autoantibodies may participate in inflammatory destruction of kidney glomerular capillaries in HFRS.
About the Authors
D. G. Ishmukhametova
Казанский государственный университет, Казань
Russian Federation
Russian Federation
B. K. Temesgen
Казанский государственный университет, Казань
Russian Federation
Russian Federation
O. V. Vlasova
Казанский государственный университет, Казань
Russian Federation
Russian Federation
F. A. Babushkina
Казанский государственный медицинский университет, Казань
Russian Federation
Russian Federation
References
1. Гармашова Н.В., Казанский В.Е., Тышкевич О.Б., Доронин Б.М., Бунева В.Н., Невинский Г.А. Антитела к ДНК в крови больных клещевым энцефалитом // Молекулярная биология. – 2004. – №29. – С.723-730.
2. Гланц С. Медико-биологическая статистика. – М.: Практика, 1999. – 459с.
3. Лакин Г.Ф. Биометрия. – М.: Высшая школа, 1990. – 352с.
4. Рязанова Г.А., Коксин В.П., Хамзина Р.В. Свободные и связанные антитела к структурным белкам ВИЧ-1 в ранний период заболевания // Медицинская иммунология. – 2005. – Т.7. – №1. – С.73-76.
5. Саттарова Л.И., Гафиятуллина Л.А., Аглиуллина Д.Г. Оптимизация иммуноферментной тестсистемы для определения антител к ДНК // Биотехнология. – 1994. – №11-12. – С.38-41.
6. Сомова-Исачкова Л.М., Плехова Н.Г. Патоморфогенез геморрагической лихорадки с почечным синдромом: от прошлого к будущему // Хантавирусы и хантавирусные инфекции. – Владивосток: ОАО «Примполиграфкомбинат», 2003. – С.182-200.
7. Christen U., von Herrath M.G. Induction, acceleration or prevention of autoimmunity by molecular mimicry // Mol. Immunol. – 2004. – Vol.40. – P.1113-1120.
8. Clifford B.D., Donahue D., Smith L., Cable E., Luttig B., Manns M., Bonkovsky H.L. High prevalence of serological markers of autoimmunity in patients with chronic hepatitis C // Hepatology. – 1995. – Vol.21. – P.613-619.
9. Goodnow C.C., Crosbie J., Adelstein S., Lavoie T.B., Smith-Gill S.J., Brink R.A., Pritchard-Briscoe H., Wotherspoon J.S., Loblay R.H., Raphael K. Altered immunoglobulin expression and functional silencing of self-reactive B lymphocytes in transgenic mice // Nature. – 1988. – Vol.334. – P. 676-682.
10. Lacroix-Desmazes S., Kaveri S.V., Mouthon L., Ayouba A., Malanchere E., Coutinho A., Kazatchkine M.D. Self-reactive antibodies in healthy individividuals // J. Immunol. Meth. – 1998. – Vol.217. – P.117-137.
11. Limaye N., Mohan C. Patogenecity of anti-DNA and glomelural autoantibodies: weighing the evidence // Drug Discovery Today: Disease Models. – 2004. – Vol.1. – Р.395-403.
12. Lin C.F., Lei H.Y., Shiau A.L., Liu C.C., Liu H.S., Yeh T.M., Chen S.H., Lin Y.S. Antibodies from dengue patient sera cross-react with endothelial cells and induce damage // J. Med. Virol. – 2003. – Vol.69. – P.82-90.
13. Misra R., Venables P.J.W., Plater-Zyberk C., Watkins P.F., Maini R.N. Anti-cardiolipin antibodies in infectious mononucleosis react with membrane of activated lymphocytes // Clin. Exp. Immunol. – 1989. – Vol.75. – P. 35-40.
14. Pawlotsky J., Yahia M., Andre C., Voisin M.C., Intrator L., Roudot-Thoraval F., Deforges L., Duvoux C., Zafrani E.S., Duval J., Dhumeaux D. Immunologic disorders in C virus chronic active hepatitis: а prospective case-control study // Hematology. – 1994. – Vol.19. – Р.841-848.
15. Vogel A., Strassburg C.P., Obermayer-Straub P., Brabant G., Manns M.P. The genetic background of autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy and its autoimmune disease components // J. Mol. Med. – 2002. – Vol.80. – P.201-211.
16. Zandman-Goddard G., Shoenfeld Yehuda. HIV and autoimmunity // Autoimmunity Reviews. – 2002. – Vol.1. – Р.329-337.
Review
For citations:
Ishmukhametova D.G., Temesgen B.K., Vlasova O.V., Babushkina F.A. AUTOANTIBODIES TO DNA IN BLOOD SERUM OF PATIENTS WITH HEMORRHAGIC FEVER WITH RENAL SYNDROME. Medical Immunology (Russia). 2006;8(5-6):653-658. (In Russ.) https://doi.org/10.15789/1563-0625-2006-5-6-653-658
Views:
1022
Email this article 