FULL-LENGTH PEPTIDE ASSAY OF ANTIGENIC PROFILE OF ENVELOPE PROTEINS FROM SIBERIAN ISOLATES OF HEPATITIS C VIRUS
https://doi.org/10.15789/1563-0625-2010-1-2-115-124
Abstract
Antigenic profiles of envelope glycoproteins of hepatitis C virus presented by three genotypes 1b, 2a/2c and 3a, which are most widespread in the territory of Russia and, in particular, in Novosibirsk, were studied using a panel of overlapping synthetic peptides. It was shown that highly immunogenic peptide epitopes of Е1 and Е2 proteins common for all HCV genotypes, are located in amino acid positions 250-260, 315-325 (Е1 protein), 390-400 (hypervariable region 1), 430-440, and 680-690 (Е2 protein). The greatest inter-genotypic differences were recorded in positions 280-290, 410-430 and 520-540. A novel antigenic determinant was detected in the region of aa 280-290 of the Е1 protein which was typical only for HCV 2a/2c genotype. A broad variation in the boundaries for the most epitopes suggests a high variability of the Е1 and Е2 viral proteins; however, a similar repertoire of antibodies induced by different HCV genotypes indicates to an opportunity of designing a new generation of cross-reactive HCV vaccines based on mapping of the E1 and E2 antigenic regions.
About the Authors
A. A. Grazhdantseva
ФГУН Государственный научный центр вирусологии и биотехнологии «Вектор» Роспотребнадзора,
Новосибирская обл., г. Кольцово
Russian Federation
Russian Federation
G. F. Sivolobova
ФГУН Государственный научный центр вирусологии и биотехнологии «Вектор» Роспотребнадзора,
Новосибирская обл., г. Кольцово
Russian Federation
Russian Federation
M. Major
Центр по оценке и исследованиям биологических препаратов, Управление по контролю за пищевыми продуктами и лекарственными средствами США, Мэриленд, г. Бетезда
Russian Federation
Russian Federation
E. V. Chub
ФГУН Государственный научный центр вирусологии и биотехнологии «Вектор» Роспотребнадзора,
Новосибирская обл., г. Кольцово
Russian Federation
Russian Federation
A. N. Shvalov
ФГУН Государственный научный центр вирусологии и биотехнологии «Вектор» Роспотребнадзора,
Новосибирская обл., г. Кольцово
Russian Federation
Russian Federation
P. V. Yudin
ФГУН Государственный научный центр вирусологии и биотехнологии «Вектор» Роспотребнадзора,
Новосибирская обл., г. Кольцово
Russian Federation
Russian Federation
S. V. Netesov
ФГУН Государственный научный центр вирусологии и биотехнологии «Вектор» Роспотребнадзора,
Новосибирская обл., г. Кольцово
ГОУ ВПО «Новосибирский государственный университет», г. Новосибирск
Russian Federation
Russian Federation
V. B. Loktev
ФГУН Государственный научный центр вирусологии и биотехнологии «Вектор» Роспотребнадзора,
Новосибирская обл., г. Кольцово
ГОУ ВПО «Новосибирский государственный университет», г. Новосибирск
Russian Federation
Russian Federation
G. V. Kochneva
ФГУН Государственный научный центр вирусологии и биотехнологии «Вектор» Роспотребнадзора,
Новосибирская обл., г. Кольцово
Russian Federation
Russian Federation
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Review
For citations:
Grazhdantseva A.A., Sivolobova G.F., Major M., Chub E.V., Shvalov A.N., Yudin P.V., Netesov S.V., Loktev V.B., Kochneva G.V. FULL-LENGTH PEPTIDE ASSAY OF ANTIGENIC PROFILE OF ENVELOPE PROTEINS FROM SIBERIAN ISOLATES OF HEPATITIS C VIRUS. Medical Immunology (Russia). 2010;12(1-2):115-124. (In Russ.) https://doi.org/10.15789/1563-0625-2010-1-2-115-124
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