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Медицинская иммунология

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ИСПОЛЬЗОВАНИЕ «SIDE POPULATION» ДЛЯ ВЫДЕЛЕНИЯ СТВОЛОВЫХ КЛЕТОК В НОРМАЛЬНЫХ И ОПУХОЛЕВЫХ ТКАНЯХ

https://doi.org/10.15789/1563-0625-2008-4-5-319-326

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Аннотация

Резюме. Анализ клеток костного мозга мышей, окрашенного флуоресцентным красителем Hoechst 33342, на проточном цитометре позволяет выявить side population (SP), которая в значительной степени обогащена гемопоэтическими стволовыми клетками, способными полностью восстанавливать кроветворение у летально облученных реципиентов. В последние годы эта методика также использовалась для анализа клеток SP в некроветворных тканях и злокачественных опухолях. Хотя фенотип и функции SP во многих тканях до конца не изучены, методика выделения SP может быть полезна для анализа и получения популяций клеток, обладающих свойствами стволовых. В данном обзоре приводится краткое описание методики и анализируются исследования SP в нормальных и опухолевых тканях.

Об авторах

О. Р. Цынкаловский
Бергенский университет, г. Берген
Норвегия
Медицинский факультет


В. А. Зайденов
Институт трансплантологии и искусственных органов Росмедтехнологий, Москва
Россия


А. С. Пахотных
Узловая железнодорожная больница, г. Тимашевск
Россия
Хирургический центр


В. А. Андрианов
Краевой нефрологический центр, г. Краснодар
Россия


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Для цитирования:


Цынкаловский О.Р., Зайденов В.А., Пахотных А.С., Андрианов В.А. ИСПОЛЬЗОВАНИЕ «SIDE POPULATION» ДЛЯ ВЫДЕЛЕНИЯ СТВОЛОВЫХ КЛЕТОК В НОРМАЛЬНЫХ И ОПУХОЛЕВЫХ ТКАНЯХ. Медицинская иммунология. 2008;10(4-5):319-326. https://doi.org/10.15789/1563-0625-2008-4-5-319-326

For citation:


Tsinkalovsky O.R., Zaidenov V.A., Pakhotnykh A.S., Andrianov V.A. EMPLOYMENT OF A «SIDE POPULATION» APPROACH TO STEM CELL ISOLATION IN NORMAL AND TUMOR TISSUES. Medical Immunology (Russia). 2008;10(4-5):319-326. (In Russ.) https://doi.org/10.15789/1563-0625-2008-4-5-319-326

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