mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2008-4-5-319-326mimmun-179Research ArticleОБЗОРЫREVIEWSИСПОЛЬЗОВАНИЕ «SIDE POPULATION» ДЛЯ ВЫДЕЛЕНИЯ СТВОЛОВЫХ КЛЕТОК В НОРМАЛЬНЫХ И ОПУХОЛЕВЫХ ТКАНЯХEMPLOYMENT OF A «SIDE POPULATION» APPROACH TO STEM CELL ISOLATION IN NORMAL AND TUMOR TISSUESЦынкаловскийО. Р.TsinkalovskyO. R.

Медицинский факультет

MD, PhD, Stem Cell Research Group, Department of Pathology

 

oleg.tsinkalovsky@gades.uib.noЗайденовВ. А.ZaidenovV. A.oleg.tsinkalovsky@gades.uib.noПахотныхА. С.PakhotnykhA. S.

Хирургический центр

oleg.tsinkalovsky@gades.uib.noАндриановВ. А.AndrianovV. A.oleg.tsinkalovsky@gades.uib.noБергенский университет, г. БергенНорвегияThe Gade Institute, Haukeland University HospitalNorwayИнститут трансплантологии и искусственных органов Росмедтехнологий, МоскваРоссияУзловая железнодорожная больница, г. ТимашевскРоссияКраевой нефрологический центр, г. КраснодарРоссия200817072014104-5319326Copyright © Цынкаловский О.Р., Зайденов В.А., Пахотных А.С., Андрианов В.А., 20142014Цынкаловский О.Р., Зайденов В.А., Пахотных А.С., Андрианов В.А.Tsinkalovsky O.R., Zaidenov V.A., Pakhotnykh A.S., Andrianov V.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/179

Резюме. Анализ клеток костного мозга мышей, окрашенного флуоресцентным красителем Hoechst 33342, на проточном цитометре позволяет выявить side population (SP), которая в значительной степени обогащена гемопоэтическими стволовыми клетками, способными полностью восстанавливать кроветворение у летально облученных реципиентов. В последние годы эта методика также использовалась для анализа клеток SP в некроветворных тканях и злокачественных опухолях. Хотя фенотип и функции SP во многих тканях до конца не изучены, методика выделения SP может быть полезна для анализа и получения популяций клеток, обладающих свойствами стволовых. В данном обзоре приводится краткое описание методики и анализируются исследования SP в нормальных и опухолевых тканях.

Abstract. A combination of fluorescent staining with Hoechst 33342 dye, and flow cytometry of murine bone marrow cells may be used for separation of a side population (SP), which is highly enriched for hematopoietic stem cells capable of long-term hematopoietic reconstitution in lethally irradiated recipients. Recently, this approach was also applied to analysis of SP cells in several types of non-hematopoietic tissues, and malignant tumours. In spite of yet poor definition of phenotype and functional potency of SP cells from various tissues, the method of SP isolation may be a useful tool for analysis and pre-enrichment of stem cell-like cells of different origin. Present review article presents a brief description of Hoechst 33342-staining approach, and of recent reports concerning SP studies in various normal and malignant tissues. (Med. Immunol., vol. 10, N 4-5, pp 319-326).

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The authors declare that there are no conflicts of interest present.