PHENOTYPIC PROFILE OF B-LYMPHOCYTES IN WOMEN WITH CHRONIC ENDOMETRITIS AND ADNEXITIS

The aim of this study was to investigate phenotypic profile of B lymphocytes in peripheral blood of the patients with chronic endometritis and adnexitis. The study involved 89 women in their reproductive age (18 to 45 years) with chronic endometritis (48 cases) and adnexitis (41 cases). Ninety-eight healthy agematched women participated as a control group. Phenotypic B-cell subpopulations were analyzed by flow cytometry performed with direct immunofluorescent staining of peripheral cells from whole blood using the following antibody panel: CD5-FITC/CD23-PE/CD19-ECD/CD45-PC5/CD27-PC7. A significantly reduced B-lymphocyte content was revealed in peripheral blood of women with chronic endometritis and adnexitis. The reduced cell numbers occurred due to reduced B2 (main fraction of B-lymphocytes) and as B1 cells (minor fraction) which determines insufficient reactivity of specific humoral immune response, including immune reactions at the mucous membranes. However, percentage of B2-lymphocytes was decreased only in endometriosis, whereas patients with adnexitis showed decrease in both relative and absolute counts of this B cell subpopulation. A decreased content of naive B-cells in the peripheral blood is another feature of the B cell phenotypic profile in chronic endometritis and adnexitis. Moreover, the drop of the naive B-cell levels in patients with adnexitis proved to be more pronounced than in persons with endometritis. Expression of CD23- antigen (a low-affinity receptor for IgE) has been investigated as a functional marker of B cells. All the studied peripheral B cell subpopulations expressing CD23 were decreased in the patients with chronic endometritis. The numbers of different B cell fractions expressing CD23 antigen were also reduced in the women with chronic adnexitis as compared to the levels detected in patients with chronic endometritis. Alterations of the B-cell immunity were more pronounced in chronic adnexitis, due to more extensive infectious/inflammatory process which involved both endometrium, and appendages, and clinical manifestation of the disease. The results suggest a need for evaluation of the B cell phenotypic profiles as a marker of inadequate immune response in infectious and inflammatory diseases of pelvic organs, as well as planning rational immunotherapy, in order to enhance therapeutic efficiency in these disorders.

B-lymphocytes, chronic endometritis, chronic adnexitis, subpopulation structure, antibacterial immunity, low-affinity IgE receptor

1. Борисов А.Г. Клиническая характеристика нарушения функции иммунной системы) // Медицинская иммунология, 2013. Т. 15, № 1. С. 45-50. [Borisov A.G. Clinical characterization of functional disorders affecting immune system. Meditsinskaya immunologiya = Medical Immunology (Russia), 2013, Vol. 15, no. 1, pp. 45-50. (In Russ.)] http://dx.doi.org/10.15789/1563-0625-2013-1-45-50

2. Кудрявцев И.В., Субботовская А.И. Опыт измерения параметров иммунного статуса с использованием шести-цветного цитофлуоримерического анализа // Медицинская иммунология, 2015. Т. 17, № 1. С. 19-26. [Kudryavtsev I.V., Subbotovskaya A.I. Application of six-color flow cytometric analysis for immune profile monitoring. Meditsinskaya immunologiya = Medical Immunology (Russia), 2015, Vol. 17, no. 1, pp. 19-26. (In Russ.)] http://dx.doi.org/10.15789/1563-0625-2015-1-19-26

3. Мальцева Л.И., Смолина Г.Р., Шарипова Р.И., Багаева М.И., Петрянина Е.В., Стовбун С.В. Хронический эндометрит в практике акушера-гинеколога // Российский вестник акушера-гинеколога, 2015. Т. 15, № 5. С. 102-105. [Maltseva L.I., Smolina G.R., Sharipova R.I., Bagaeva M.I., Petrjanina E.V., Stovbun S.V. Chronic endometritis in the practice of an obstetrician/gynecologist. Rossiyskiy vestnik akushera-ginekologa = Russian Bulletin of Obstetrician-Gynecologist, 2015, Vol. 15, no. 5, pp. 102-105. (In Russ.)]

4. Муравьева В.В., Припутневич Т.В., Якушевская О.В., Чернуха Г.Е., Марченко Л.А., Анкирская А.С., Любасовская Л.А., Мелкумян А.Р. Роль условно-патогенных микроорганизмов в этиологии хронического эндометрита у женщин репродуктивного возраста // Клиническая микробиология и антимикробная химиотерапия, 2015. Т. 17, № 4. С. 318-327. [Muravyeva V.V., Priputnevich T.V., Yakushevskaya O.V., Chernukha G.E., Marchenko L.A., Ankirskaya A.S., Ljubasovskaya L.A., Melkumian A.R. The role of the opportunistic pathogens in the etiology of chronic endometritis in women of reproductive age. Klinicheskaya mikrobiologiya i antimikrobnaya khimioterapiya = Clinical Microbiology and Antimicrobial Chemotherapy, 2015, Vol. 17, no. 4, pp. 318-327. (In Russ.)]

5. Хайдуков С.В., Байдун Л.А., Зурочка А.В., Тотолян Арег А. Стандартизованная технология «Исследование субпопуляционного состава лимфоцитов периферической крови с применением проточных цитофлюориметров-анализаторов» (Проект) // Медицинская иммунология, 2012. Т. 14, № 3. С. 255-268. [Khaydukov S.V., Baydun L.A., Zurochka A.V., Totolian Areg A. Standardized technology «Research of lymphocytes subpopulation composition in peripheral blood using flow cytometry analyzers» (Draft). Meditsinskaya immunologiya = Medical Immunology (Russia), 2012, Vol. 14, no. 3, pp. 255-268. (In Russ.)] http://dx.doi.org/10.15789/1563-0625-2012-3-255-268

6. Amezcua Vesely M.C., Schwartz M., Bermejo D.A., Montes C.L., Cautivo K.M., Kalergis A.M., Rawlings D.J., Acosta-Rodríguez E.V., Gruppi A. FcγRIIb and BAFF differentially regulate peritoneal B1 cell survival. J. Immunol., 2012, Vol. 188, no. 10, pp. 4792-4800.

7. Beruchashvili M., Gogiashvili L., Datunashvili E., Topuria Z., Tsagareli Z. Morphological peculiarities of endometrium in chronic endometritis associated with bacterial vaginosis. Georgian Med. News, 2010, Vol. 181, pp. 59-63.

8. Braza F., Chesne J., Durand M., Dirou S., Brosseau C., Mahay G., Cheminant M.A., Magnan A., Brouard S. A regulatory CD9(+) B-cell subset inhibits HDM-induced allergic airway inflammation. Allergy, 2015, Vol. 70, no. 11, pp. 1421-1431.

9. Kitaya K., Yasuo T. Aberrant expression of selectin E, CXCL1, and CXCL13 in chronic endometritis. Mod. Pathol., 2010, Vol. 23, no. 8, pp. 1136-1146.

10. Luider J.1., Cyfra M., Johnson P., Auer I. Impact of the new Beckman Coulter Cytomics FC 500 5-color flow cytometer on a regional flow cytometry clinical laboratory service. Lab. Hematol., 2004, Vol. 10, pp. 102-108.

11. Maecker H., McCoy P., Nussenblatt R. Standardizing immunophenotyping for the human immunology project. Nat. Rev. Immunol., 2012, Vol. 12, pp. 191-200.

12. Pittrof R., Sully E., Bass D.C., Kelsey S.F., Ness R.B., Haggerty C.L. Stimulating an immune response? Oral sex is associated with less endometritis. Int. J. STD AIDS, 2012, Vol. 23, no. 11, pp. 775-780.

13. Pore D., Matsui K., Parameswaran N., Gupta N. Cutting Edge: Ezrin Regulates Inflammation by Limiting B Cell IL-10 Production. J. Immunol., 2016, Vol. 196, no. 2, pp. 558-562.

14. Potter T.J., Guitian J., Fishwick J., Gordon P.J., Sheldon I.M. Risk factors for clinical endometritis in postpartum dairy cattle. Theriogenology, 2010, Vol. 74, no. 1, pp. 127-134.

15. Sutton B.J., Davies A.M. Structure and dynamics of IgE-receptor interactions: FcεRI and CD23/FcεRII. Immunol. Rev., 2015, Vol. 268, no. 1, pp. 222-235.