mimmunМедицинская иммунологияMedical Immunology (Russia)1563-06252313-741XSPb RAACI10.15789/1563-0625-2016-4-379-384mimmun-1061Research ArticleКРАТКИЕ СООБЩЕНИЯSHORT COMMUNICATIONSФЕНОТИПИЧЕСКИЙ СОСТАВ В-ЛИМФОЦИТОВ У ЖЕНЩИН С ХРОНИЧЕСКИМ ЭНДОМЕТРИТОМ И АДНЕКСИТОМPHENOTYPIC PROFILE OF B-LYMPHOCYTES IN WOMEN WITH CHRONIC ENDOMETRITIS AND ADNEXITISСавченкоА. А.SavchenkoA. A.

д.м.н., профессор, руководитель лаборатории молекулярно-клеточной физиологии и патологии,

г. Красноярск

PhD, MD (Medicine), Professor, Head, Laboratory of Molecular and Cellular Physiology and Pathology,

Krasnoyarsk

КаспаровЭ. В.KasparovE. V.

д.м.н., профессор, директор,

г. Красноярск

PhD, MD (Medicine), Professor, Director,

Krasnoyarsk

АрутюнянС. С.ArutyunyanS. S.

аспирант,

г. Красноярск

Postgraduate Student,

Krasnoyarsk

БорисовА. Г.BorisovA. G.

к.м.н., ведущий научный сотрудник лаборатории молекулярно-клеточной физиологии и патологии,

г. Красноярск

PhD (Medicine), Leading Research Associate, Laboratory of Molecular and Cellular Physiology and Pathology,

Krasnoyarsk

КудрявцевИ. В.KudryavtsevI. V.

к.м.н., старший научный сотрудник отдела иммунологии,

197376, Санкт-Петербург, ул. акад. Павлова, 12

PhD (Medicine), Senior Research Associate, 197376, Russian Federation, St. Petersburg, Acad. Pavlov str., 12;

Vladivostok;

St. Petersburg

 

igorek1981@yandex.ruМошевА. В.MoshevA. V.

младший научный сотрудник лаборатории молекулярно-клеточной физиологии и патологии,

г. Красноярск

Junior Research Associate, Laboratory of Molecular and Cellular Physiology and Pathology,

Krasnoyarsk

ФГБНУ «Научно-исследовательский институт медицинских проблем Севера»; ГБОУ ВПО «Красноярский государственный медицинский университет им. профессора В.Ф. Войно-Ясенецкого» МЗ РФРоссияResearch Institute of Medical Problems of the North; Krasnoyarsk V.F. Voino-Yasenetsky State Medical UniversityRussian FederationФГБНУ «Научно-исследовательский институт медицинских проблем Севера»РоссияResearch Institute of Medical Problems of the NorthRussian FederationФГБНУ «Институт экспериментальной медицины»; ФГАОУ ВПО «Дальневосточный федеральный университет»; ГБОУ ВПО «Первый Санкт-Петербургский государственный медицинский университет имени академика И.П. Павлова» Министерства здравоохранения РФРоссияResearch Institute of Experimental Medicine; Far Eastern Federal University; Pavlov First St. Petersburg State Medical UniversityRussian Federation201623082016184379384Copyright © Савченко А.А., Каспаров Э.В., Арутюнян С.С., Борисов А.Г., Кудрявцев И.В., Мошев А.В., 20162016Савченко А.А., Каспаров Э.В., Арутюнян С.С., Борисов А.Г., Кудрявцев И.В., Мошев А.В.Savchenko A.A., Kasparov E.V., Arutyunyan S.S., Borisov A.G., Kudryavtsev I.V., Moshev A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.mimmun.ru/mimmun/article/view/1061

The aim of this study was to investigate phenotypic profile of B lymphocytes in peripheral blood of the patients with chronic endometritis and adnexitis. The study involved 89 women in their reproductive age (18 to 45 years) with chronic endometritis (48 cases) and adnexitis (41 cases). Ninety-eight healthy agematched women participated as a control group. Phenotypic B-cell subpopulations were analyzed by flow cytometry performed with direct immunofluorescent staining of peripheral cells from whole blood using the following antibody panel: CD5-FITC/CD23-PE/CD19-ECD/CD45-PC5/CD27-PC7. A significantly reduced B-lymphocyte content was revealed in peripheral blood of women with chronic endometritis and adnexitis. The reduced cell numbers occurred due to reduced B2 (main fraction of B-lymphocytes) and as B1 cells (minor fraction) which determines insufficient reactivity of specific humoral immune response, including immune reactions at the mucous membranes. However, percentage of B2-lymphocytes was decreased only in endometriosis, whereas patients with adnexitis showed decrease in both relative and absolute counts of this B cell subpopulation. A decreased content of naive B-cells in the peripheral blood is another feature of the B cell phenotypic profile in chronic endometritis and adnexitis. Moreover, the drop of the naive B-cell levels in patients with adnexitis proved to be more pronounced than in persons with endometritis. Expression of CD23- antigen (a low-affinity receptor for IgE) has been investigated as a functional marker of B cells. All the studied peripheral B cell subpopulations expressing CD23 were decreased in the patients with chronic endometritis. The numbers of different B cell fractions expressing CD23 antigen were also reduced in the women with chronic adnexitis as compared to the levels detected in patients with chronic endometritis. Alterations of the B-cell immunity were more pronounced in chronic adnexitis, due to more extensive infectious/inflammatory process which involved both endometrium, and appendages, and clinical manifestation of the disease. The results suggest a need for evaluation of the B cell phenotypic profiles as a marker of inadequate immune response in infectious and inflammatory diseases of pelvic organs, as well as planning rational immunotherapy, in order to enhance therapeutic efficiency in these disorders.

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The authors declare that there are no conflicts of interest present.