SERUM SECRETORY IMMUNOGLOBULIN A AND Gln223Arg POLYMORPHISM OF THE LEPR GENE IN ALCOHOLIC AND NON-ALCOHOLIC FATTY LIVER DISEASES
https://doi.org/10.15789/1563-0625-2014-5-465-472
Abstract
Level of serum secretory immunoglobulin A was investigated in alcoholic liver disease (ALD, 59 men and 23 women) and non-alcoholic fatty liver disease (NAFLD, 17 men and 93 women).The data were compared with those of persons without liver pathology (control group, 43 men and 73 women). Moreover, we studied possible associations between ssIgA level and Gln223Arg polymorphism of the LEPR gene. Immunological and DNA diagnostics was performed by means of, respectively, ELISA and allele-specific polymerase chain reaction. We have found that the average level of ssIgA was three times higher in ALD group (11.45±0.82 mg/l), than in the NAFLD group (4.35±0.35 mg/l) or in controls (3,60±0,29 mg/l). SsIgА concentration did not depend on adiposity and gender. The ssIgА concentration proved to be increased in Gln223Arg heterozygotes with NAFLD, when compared with controls. However, the frequency of 223Arg and 223Gln alleles was virtually equal in all observed groups with above-normal concentration of ssIgА, as compared to a sub-group with normal ssIgА concentration. Hence, we have not revealed any significant association between Gln223Arg polymorphism of LEPR and ssIgA level. The data obtained will be useful for studying genetic risk factors in development of infectious mucosаl lesions.
About the Authors
N. V. Mal’tseva
Novokuznetsk State Institute of Postgraduate Medicine, Novokuznetsk, Russian Federation
Russian Federation
Russian Federation
PhD, MD, Chief, Research Laboratory of Molecular Biology, Novokuznetsk State Institute of Postgraduate Medicine 654005, Russian Federation, Novokuznetsk, Stroitelei av., 5. Phone: 7 (3843) 45-56-41. Fax: 7 (3843) 45-42-19
O. F. Lykova
Novokuznetsk State Institute of Postgraduate Medicine, Novokuznetsk, Russian Federation
Russian Federation
Russian Federation
PhD (Biology), Leading Research Associate, Research Laboratory of Molecular Biology, Novokuznetsk State Institute of Postgraduate Medicine, Novokuznetsk, Russian Federation
A. V. Morozova
Novokuznetsk State Institute of Postgraduate Medicine, Novokuznetsk, Russian Federation
Russian Federation
Russian Federation
PhD Candidate, Department of Therapy, Novokuznetsk State Institute of Postgraduate Medicine, Novokuznetsk, Russian Federation
S. V. Arkhipova
Novokuznetsk State Institute of Postgraduate Medicine, Novokuznetsk, Russian Federation
Russian Federation
Russian Federation
PhD (Medicine), Research Laboratory of Biochemistry, Novokuznetsk State Institute of Postgraduate Medicine, Novokuznetsk, Russian Federation
Ya. A. Gorbatovskii
Novokuznetsk State Institute of Postgraduate Medicine, Novokuznetsk, Russian Federation
Russian Federation
Russian Federation
PhD, MD (Medicine), Professor, Chief, Department of Therapy, Novokuznetsk State Institute of Postgraduate Medicine, Novokuznetsk, Russian Federation
References
1. Кишкун А.А. Руководство по лабораторным методам диагностики. М.: ГЭОТАР-Медиа, 2009. 800 с. Kishkun A.A. Rukovodstvo po laboratornym metodam diagnostiki [Management on laboratory methods of diagnostics]. Мoscow: GEOTAR-Media, 2009. 800 p.]
2. Кукайн Э.М., Хазенсон Л.Б., Рыбкин М.П., Лосева А.Г., Боброва О.И. Секреторный иммуноглобулин А в сыворотках крови здоровых и больных людей // Журнал микробиологии эпидемиологии и иммунологии. 1977. № 8. С. 106-111. Kukayn E.M., Hazenson L.B., Rybkin M.P., Loseva A.G., Bobrova O.I. ekretornyy immunoglobulin A v syvorotkakh krovi zdorovykh i bol`nykh lyudey [Secretory immunoglobulin A in blood sera of healthy and sick people]. Zhurnal mikrobiologii epidemiologii i immunologii = Journal of Microbiology, Epidemiology and Immunology, 1977, no. 8, pp. 106-111].
3. Ройт А., Бростофф Дж., Мейл Д. Иммунология: Пер. с англ. М.: Мир, 2000. 592 с. Royt A., Brostoff Dzh., Meyl D. Immunologiya. Рer. s angl. [Immunology. Engl. Transl.] Moscow: Mir, 2000. 592 p.]
4. Duggal P., Guo X., Haque R., Peterson K.M., Ricklefs S., Mondal D., Alam F., Noor Z., Verkerke H.P., Marie Ch., Leduc Ch.A., ChPetri Jr. W.A. A mutation in the leptin receptor is associated with Entamoeba histolytica infection in children. J. Clin. Invest., 2011, Vol. 121, no. 3, pp. 1191-1198.
5. Iorio R., Sepe A., Giannattasio A., Cirillo F., Spagnuolo M.I., Franzese A., Fontana S., Aufiero D., Perna F., Vegnente A., Matarese G. Immune phenotype and serum leptin in children with obesity-related liver disease. J. Clin. Endocrinol. Metab., 2006, Vol. 91, no. 1, pp. 341-344.
6. Gonz lez-Quintela A., Alende M.R., Gamallo R., Gonz lez-Gil P., L pez-Ben S., Tomé S., Otero E., Torre J.A. Serum immunoglobulins (IgG, IgA, IgM) in chronic hepatitis C. A comparison with non-cirrhotic alcoholic liver disease. Hepatogastroenterology, 2003, Vol. 50, no. 54, pp. 2121-2126.
7. Okamatsu Y., Matsuda K., Hiramoto I., Tani H., Kimura K., Yada Y., Kakuma T., Higuchi S., Kojima M., Matsuishi T. Ghrelin and leptin modulate immunity and liver function in overweight children. Pediatr. Int., 2009, Vol. 51, no. 1, pp. 9-13.
8. Tomita K., Teratani T., Yokoyama H., Suzuki T., Irie R., Ebinuma H., Saito H., Hokari R., Miura S., Hibi T. Serum immunoglobulin concentration is an independent predictor of liver fibrosis in nonalcoholic teatohepatitis before the cirrhotic stage. Dig. Dis. Sci., 2011, Vol. 56, no. 12, pp. 3648-3654.
9. van de Wiel A., van Hattum J., Schuurman H.J., Kater L. Immunoglobulin A in the diagnosis of alcoholic liver disease. Gastroenterology, 1988, Vol. 94, no. 2, pp. 457-462.
Review
For citations:
Mal’tseva N.V., Lykova O.F., Morozova A.V., Arkhipova S.V., Gorbatovskii Ya.A. SERUM SECRETORY IMMUNOGLOBULIN A AND Gln223Arg POLYMORPHISM OF THE LEPR GENE IN ALCOHOLIC AND NON-ALCOHOLIC FATTY LIVER DISEASES. Medical Immunology (Russia). 2014;16(5):465-472. (In Russ.) https://doi.org/10.15789/1563-0625-2014-5-465-472
Views:
1429
Email this article 