NOVEL METHODS FOR AUTOANTIBODY DETECTION IN LABORATORY DIAGNOSTICS OF AUTOIMMUNE RHEUMATIC DISEASES
https://doi.org/10.15789/1563-0625-2014-3-221-226
Abstract
Autoantibodies are well-established biomarkers of autoimmune rheumatic diseases. Their detection in the routine clinical laboratory provides key information for early and differential diagnosis, prognosis, and monitoring of disease-activity. However, the currently existing variety of laboratory tests and methods as well as different diagnostic and clinical algorithms of autoimmune testing is the reason for lack of reproducibility and harmonization across immunological laboratories. Novel technical developments in the area of digital analysis of immunofluorescent images paved the way for the improvement of intra- and interlaboratory standardizationof test results. Additionally, digital immunofluorescence analysis enables the simultaneous multiparametric combination of cell- or tissue-based indirect immunofluorescence screening tests with confirmatory testing employing microparticles covered with purified autoantigens in one reaction environment.
About the Authors
D. Roggenbuck
Brandenburg Technical University Cottbus-Senftenberg, Senftenberg, Germany
Russian Federation
Russian Federation
PhD, MD (Medicine), Professor, Faculty of Science,
Brandenburg Technical University Cottbus-Senftenberg
P. Schierac
Brandenburg Technical University Cottbus-Senftenberg, Senftenberg, Germany
Russian Federation
Russian Federation
PhD, MD (Medicine), Professor, Faculty of Science,
Brandenburg Technical University Cottbus-Senftenberg
U. Sack
Institute of Translational and Regenerative Medicine, University of Leipzig, Germany
Russian Federation
Russian Federation
PhD, MD (Medicine), Professor, Institute of Translational
and Regenerative Medicine, University of Leipzig
S. V. Lapin
St. Petersburg Pavlov State Medical University, St. Petersburg
Russian Federation
Russian Federation
PhD (Medicine), Сhief, Laboratory of Autoimmune
Diagnostics, St. Petersburg Pavlov State Medical University
A. V. Mazing
St. Petersburg Pavlov State Medical University, St. Petersburg
Russian Federation
Russian Federation
PhD (Medicine), Senior Research Associate,
Laboratory of Autoimmune Diagnostics, St. Petersburg Pavlov State Medical University
Areg A. Totolian
St. Petersburg Pasteur Institute of Epidemiology and Microbiology, St. Petersburg
Almazov Federal Medical Research Centre, St. Petersburg
Russian Federation
Russian Federation
PhD, MD (Medicine), Professor, Corresponding Member of RAMS, Deputy Director, St. Petersburg Pasteur Institute of
Epidemiology and Microbiology; Head, Laboratory of Immunology, Almazov Federal Medical Research Centre
References
1. Lasareva N.M., Lapin S.V., Mazing A.V., Bulgakova T.V., Ilivanova E.P., Maslyanskiy A.L. Optimizatsiya kompleksa serologicheskikh metodov diagnostiki sistemnykh zabolevaniy soedinitel`noy tkani [Optimization of complex of serological methods for diagnosis of systemic connective tissue diseases]. Klinicheskaya laboratornaya diagnostika – Clinical Laboratory Diagnostics, 2011, vol. 12, pp. 12-17.
2. Lapin S.V., Totolian A.A. Antinuklearnye antitela: laboratornye testy i diagnosticheskoe znachenie [Antinuclear antibody: laboratory tests and clinical significance]. Meditsinskaya immunologiya – Medical Immunology, 2001, vol. 3, no. 1, pp. 35-50.
3. Lapin S.V., Totolian A.A. Diagnostika autoimmunnykh zabolevaniy s pomoshch`yu metoda nepryamoy immunoflyuorestsentsii [DIagnostics of autoimmune diseases with indirect immunofluorascence]. Meditsinskaya immunologiya – Medical Immunology, 2000, vol. 2, no. 2, pp. 174.
4. Lapin S.V., Totolian A.A. Immunologicheskaya diagnostika zabolevaniy pochek pri vaskulitakh [Immunolgical diagnostics of kidney involvment in vasculitis]. Nephrologia – Nephrology, 2003, vol. 16, no. 4, pp. 39-44.
5. Sozina A.V., Neustroeva Yu.A., Tikhomirova T.A., Lapin S.V. Sochetannaya vstrechaemost` autoantitel u bol`nykh s diffuznymi boleznyami soedinitel`noy tkani [Сoincidence of autoantibodies among patients with diffuse connective tissue disorders]. Meditsinskaya immunologiya – Medical Immunology, 2007, vol. 9, no. 1, pp. 69-76.
6. Agmon-Levin N., Damoiseaux J., Kallenberg C., Sack U., Witte T., Herold M., Bossuyt X., Musset L., Cervera R., Plaza-Lopez A., Dias C., Sousa M. J., Radice A., Eriksson C., Hultgren O., Viander M., Khamashta M., Regenass S., Andrade L.E., Wiik A., Tincani A., Ronnelid J., Bloch D.B., Fritzler M.J., Chan E.K., Garcia-De La Torre I., Konstantinov K.N., Lahita R., Wilson M., Vainio O., Fabien N., Sinico R.A., Meroni P., Shoenfeld Y. International recommendations for the assessment of autoantibodies to cellular antigens referred to as anti-nuclear antibodies. Ann. Rheum. Dis., 2014, vol. 73, no. 1, pp. 17-23.
7. Bossuyt X., Cooreman S., De Baere H., Verschueren P., Westhovens R., Blockmans D., Marien G. Detection of antinuclear antibodies by automated indirect immunofluorescence analysis. Clin. Chim. Acta, 2013, vol. 415, pp. 101-106.
8. Conrad K., Roggenbuck D., Reinhold D., Sack U. Autoantibody diagnostics in clinical practice. Autoimmun. Rev., 2012, vol. 11, no. 3, pp. 207-211.
9. Conrad K., Sack U. Multiparametric analyses in diagnostics and monitoring of autoimmune diseases: current state and perspectives. Laboratoriumsmedizin – Journal of Laboratory Medicine, 2011, vol. 35, pp. 375-382.
10. Czaja A.J., Freese D.K. Diagnosis and treatment of autoimmune hepatitis. Hepatology, 2002, vol. 36, no. 2, pp. 479-497.
11. Damoiseaux J. Autoantibodies in the grocery shop: does quantity matter? Immunol. Res., 2013, vol. 56, no. 2-3, pp. 413-419.
12. Egerer K., Roggenbuck D., Hiemann R., Weyer M.G., Buttner T., Radau B., Krause R., Lehmann B., Feist E., Burmester G.R. Automated evaluation of autoantibodies on human epithelial-2 cells as an approach to standardize cell-based immunofluorescence tests. Arthritis Res. Ther., 2010, vol. 12, no. 2, p. R40.
13. Fritzler M.J. Challenges to the use of autoantibodies as predictors of disease onset, diagnosis and outcomes. Autoimmun. Rev., 2008, vol. 7, no. 8, pp. 616-620.
14. Hiemann R., Buttner T., Krieger T., Roggenbuck D., Sack U., Conrad K.Challenges of automated screening and differentiation of non-organ specific autoantibodies on HEp-2 cells. Autoimmun. Rev., 2009, vol. 9, no. 1, pp. 17-22.
15. Hiemann R., Hilger N., Michel J., Nitschke J., Bohm A., Anderer U., Weigert M., Sack U. Automatic analysis of immunofluorescence patterns of HEp-2 cells. Ann. N Y Acad. Sci., 2007, vol. 1109, pp. 358-371.
16. Kivity S., Gilburd B., Agmon-Levin N., Carrasco M.G., Tzafrir Y., Sofer Y., Mandel M., Buttner T., Roggenbuck D., Matucci-Cerinic M., Danko K., Hoyos M.L., Shoenfeld Y. A novel automated indirect immunofluorescence autoantibody evaluation. Clin. Rheumatol., 2012, vol. 31, no. 3, pp. 503-509.
17. Knutter I., Hiemann R., Brumma T., Buttner T., Grossmann K., Cusini M., Pregnolato F., Borghi M.O., Anderer U., Conrad K., Reinhold D., Roggenbuck D., Csernok E.Automated interpretation of ANCA patterns – a new approach in the serology of ANCA-associated vasculitis. Arthritis Res. Ther., 2012, vol. 14, no. 6, p. R271.
18. Meroni P.L., Borghi M.O., Raschi E., Tedesco F. Pathogenesis of antiphospholipid syndrome: understanding the antibodies. Nat. Rev. Rheumatol., 2011, vol. 7, no. 6, pp. 330-339.
19. Meroni P.L., Schur P.H. ANA screening: an old test with new recommendations. Ann. Rheum. Dis., 2010, vol. 69, no. 8, pp. 1420-1422.
20. Pakozdi A., Nihtyanova S., Moinzadeh P., Ong V.H., Black C.M., Denton C.P. Clinical and serological hallmarks of systemic sclerosis overlap syndromes. J. Rheumatol., 2011, vol. 38, no. 11, pp. 2406-2409.
21. Roggenbuck D., Hiemann R., Bogdanos D., Reinhold D., Conrad K. Standardization of automated interpretation of immunofluorescence tests. Clin. Chim. Acta, 2013, vol. 421, pp. 168-169.
22. Roggenbuck D., Reinhold D., Hiemann R., Anderer U., Conrad K.Standardized detection of anti-ds DNA antibodies by indirect immunofluorescence – a new age for confirmatory tests in SLE diagnostics. Clin. Chim. Acta, 2011, vol. 412, no. 21-22, pp. 2011-2012.
23. Savige J., Davies D., Falk R.J., Jennette J.C., Wiik A. Antineutrophil cytoplasmic antibodies and associated diseases: a review of the clinical and laboratory features. Kidney Int., 2000, vol. 57, no. 3, pp. 846-862.
24. Savige J., Dimech W., Fritzler M., Goeken J., Hagen E.C., Jennette J.C., McEvoy R., Pusey C., Pollock W., Trevisin M., Wiik A., Wong R. Addendum to the International Consensus Statement on testing and reporting of antineutrophil cytoplasmic antibodies. Quality control guidelines, comments, and recommendations for testing in other autoimmune diseases. Am. J. Clin. Pathol., 2003, vol. 120, no. 3, pp. 312-318.
25. Tozzoli R., Antico A., Porcelli B., Bassetti D. Automation in indirect immunofluorescense testing: a new step in the evolution of the autoimmunology laboratory. Autoimmun. Highlights, 2012, vol. 3, pp. 59-65.
26. van Venrooij W.J., Zendman A.J., Pruijn G.J. Autoantibodies to citrullinated antigens in (early) rheumatoid arthritis. Autoimmun. Rev., 2006, vol. 6, no. 1, pp. 37-41.
27. Wiik A. Autoantibodies in vasculitis. Arthritis Res. Ther., 2003, vol. 5, no. 3, pp. 147-152.
28. Wiik A., Cervera R., Haass M., Kallenberg C., Khamashta M., Meroni P.L., Piette J.C., Schmitt R., Shoenfeld Y. European attempts to set guidelines for improving diagnostics of autoimmune rheumatic disorders. Lupus, 2006, vol. 15, no. 7, pp. 391-396.
29. Wiik A.S., Gordon T.P., Kavanaugh A.F., Lahita R.G., Reeves W., van Venrooij W.J., Wilson M.R., Fritzler M. Cutting edge diagnostics in rheumatology: the role of patients, clinicians, and laboratory scientists in optimizing the use of autoimmune serology. Arthritis Rheum., 2004, vol. 51, no. 2, pp. 291-298.
30. Willitzki A., Lorenz S., Hiemann R., Guttek K., Goihl A., Hartig R., Conrad K., Feist E., Sack U., Schierack P., Heiserich L., Eberle C., Peters V., Roggenbuck D., Reinhold D. Fully automated analysis of chemically induced gammaH2AX foci in human peripheral blood mononuclear cells by indirect immunofluorescence. Cytometry A, 2013, vol. 83, no. 11, pp. 1017-1026.
Review
For citations:
Roggenbuck D., Schierac P., Sack U., Lapin S.V., Mazing A.V., Totolian A.A. NOVEL METHODS FOR AUTOANTIBODY DETECTION IN LABORATORY DIAGNOSTICS OF AUTOIMMUNE RHEUMATIC DISEASES. Medical Immunology (Russia). 2014;16(3):221-226. (In Russ.) https://doi.org/10.15789/1563-0625-2014-3-221-226
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