CLINICAL AND DIAGNOSTIC SIGNIFICANCE OF DEOXYRIBONUCLEASE ACTIVITY OF POLYCLONAL IgG AND BLOOD SERA IN PATIENTS WITH SPONDYLOARTHRITIS
https://doi.org/10.15789/1563-0625-2012-4-5-337-346
Abstract
Abstract. A group of 266 patients with spondyloarthritidis and 69 healthy persons were included in our study. IgG preparations were isolated from blood sera by a combined rivanol/affine chromatography technique. Homogeneity of IgGs was tested by means of SDS-PAGE. Serum samples from patients and healthy persons, and IgG subclasses 1, 2 and 4 were tested for DNAse activity. A method of DNAse activity measurement was based on rivanol capacity to form a clot with DNA. We have found highly significant differences between the levels of DNAse activity associated with IgG preparations and in blood sera from patients with spondyloarthritidis, and healthy donors (p < 0,0001). DNAse activity of IgG and sera in patients with psoriatic arthritis was higher than in patients with reactive arthritis and ankylosing spondylitis (p < 0,0001). Multiple correlations were revealed between DNAse activity of IgG, blood serum, clinical signs of psoriatic arthritis, reactive arthritis, ankylosing spondylitis, and laboratory findings. We have developed novel tests for differential diagnosis between various disorders, e.g., spondyloarthritidis, based on IgG and serum-associated DNAse activity, corresponding to the criteris of useful and very useful diagnostic tests in rheumatology.
About the Authors
E. V. Kunder
Государственное учреждение образования «Белорусская медицинская академия последипломного
образования», г. Минск
Belarus
Belarus
M. V. Volkova
Учреждение образования «Витебский государственный медицинский университет», г. Витебск
Belarus
Belarus
I. I. Generalov
Учреждение образования «Витебский государственный медицинский университет», г. Витебск
Belarus
Belarus
G. A. Nevinsky
Учреждение Российской академии наук «Институт химической биологии и фундаментальной медицины» Сибирского отделения РАН, г. Новосибирск
Russian Federation
Russian Federation
References
1. Генералов И.И., Сидорская Е.В. Абзимная активность препаратов IgG у больных ревматоидным артритом и системной красной волчанкой // Иммунология. – 1998. – № 3. – С. 54-56.
2. Мальцев К.А., Хитров А.Н., Введенская О.Ю., Пономаренко Н.А., Исаева М.А., Кимова М.В., Третьяк Е.Б., Шогенов З.С., Алекберова З.С., Габибов А.Г., Сучков С.В. Каталитические аутоантитела – новый молекулярный инструмент в кардиологии и офтальмологии // Тер. архив. – 2006. – № 11. – С. 70-76.
3. Насонов Е.Л., Александрова Е.Н. Современные стандарты лабораторной диагностики ревматических заболеваний. Москва, 2006. – 70 с.
4. Невинский Г.А., Канышкова Т.Г., Бунева В.Н. Природные каталитически активные антитела (абзимы) в норме и при патологии // Биохимия. – 2000. – № 65. – С. 1245-1255.
5. Способ определения ДНКазной активности: пат. 243А Респ. Беларусь, МПК С12 Q1/34, С 12 N 9/22 / К.С. Азаренок, И.И. Генералов, А.Г. Голубева, Н.В. Железняк, М.Р. Конорев; 1996. – № 3. – С. 174.
6. Способ очистки иммуноглобулинов класса G из сыворотки крови и устройство для аффинной хроматографии: пат. 3205 Респ. Беларусь, МПК 6G 01 N 33/48 / М.Р. Конорев, И.И. Генералов, И.В. Жильцов, А.Г. Генералова; 1999. – № 3. – С. 121.
7. Хитров А.Н. ДНК-абзимы при ревматоидном артрите: патогенетическое и клиническое значение // Тер. архив. – 2005. – № 77. – С. 75-80.
8. Akkoc N. Are spondyloarthropathies as common as rheumatoid arthritis worldwide? // Curr. Rheumatol. Rep. – 2008. – Vol. 10. – P. 371-378.
9. Bergman M.J. Social and economic impact of inflammatory arthritis // Postgrad. Med. – 2006. – Vol. 5. – P. 8-11.
10. Braun J., Kingsley G., van der Heijde D., Sieper J. On the difficulties of establishing a consensus on the definition of and diagnostic investigations for reactive arthritis. Results and discussion of a questionnaire prepared for the 4th International Workshop on Reactive arthritis // J. Rheumatol. – 2000. – Vol. 27. – P. 2185-2192.
11. Calzavara-Pinton P.G., Franceschini F., Manera C. Incidence of antiperinuclear factor in patients with psoriatic arthritis // Adv. Exp. Med. Biol. – 1999. – Vol. 455. – P. 215-220.
12. Chen C.H., Yu D.T., Chou C.T. Biomarkers in spondyloarthropathies // Adv. Exp. Med. Biol. – 2009. – Vol. 649. – P. 122-132.
13. Dougados M., van der Linder S., Juhlin R., Huitfeldt B., Amor B., Calin A., Cats A., Dijkmans B., Olivieri I., Pasero G. The European Spondyloarthropathy Study Group preliminary criteria for classification of spondyloarthropathy // Arthritis Rheum. – 1991. – Vol. 34. – P. 1218-1227.
14. Healy P.J., Helliwell P.S. Classification of the spondyloarthropathies // Curr. Opin. Rheumatol. – 2005. – Vol. 17. – P. 393-395.
15. Johnson S.R., Schentag C.T., Gladman D.D. Autoantibodies in biological agents na ve patients with psoriatic arthritis // Ann. Rheum.Dis. – 2005. – Vol. 64. – P. 770-772.
16. Kavanaugh A., Solomon D.H., Schur P., American college of rheumatology ad hoc committee on immunologic testing guidelines Guidelines for immunologic laboratory testing in the rheumatic diseases: an introduction // Arthritis Rheum. – 2002. – Vol. 47. – P. 429-433.
17. Kawai Y., Yoshida M., Arawaka K., Kumamoto T., Morikawa N., Masamura K., Tada H., Ito S., Hoshizaki H., Oshima S., Taniguchi K., Terasawa H., Miyamori I., Kishi K., Yasuda T. Diagnostic Use of Serum Deoxyribonuclease I Activity as a Novel Early-Phase Marker in Acute Myocardial Infarction // Circulation. – 2004. – Vol. 109. – P. 2398-2400.
18. Kozyr A.V., Kolesnikov A.V., Zelenova N.A., Sashenko L.P., Mikhalap S.V., Bulina M.E., Ignatova A.N., Favorov P.V., Gabibov A.G. Autoantibodies to nuclear antigens: correlation between cytotoxicity and DNA-hydrolyzing activity // Appl. Biochem Biotechnol. – 2000. – Vol. 83. – P. 255-268.
19. Rader C., List B. Catalytic antibodies as magic bullets // Chemistry. – 2000. – Vol. 12. – P. 2091-2095.
20. Sallai K., Nagy E., Derfalvy B., Muzes G., Gergely P. Antinucleosome antibodies and decreased deoxyribonuclease activity in sera of patients with systemic lupus erythematosus // Clinical and93-398. Diagnostic Laboratory Immunology. – 2005. – Vol. 1. – P. 56-59.
21. Shojania K. What laboratory tests are needed? // CMAJ. – 2000. – Vol. 162. – P. 743-746.
22. Suchkov S.V., Gabibiv A.G., Gnuchev N.V. Phenomenon of DNA-abzyme cross-reactivity and its significance for the mechanisms of cytotoxicity and apoptosis // Ontogenez. – 2001. – Vol. 32. – P. 348-352.
23. Taylor W., Gladman D.D., Helliwell P., Marchesoni A., Mease P., Mielants H. CASPAR Study Group. Classification criteria for psoriatic arthritis: development of new criteria from a large international study // Ann. Rheum. Dis. – 2006. – Vol. 54. – P. 2665-2673.
24. Van der Linder S., Valkenburg H.A., Cat Valkenburgs A. Evaluation of diagnostic criteria for ankylosing spondylitis: a proposal for modification of the New York criteria // Arthritis Rheum. – 1984. – Vol. 2. – P. 361-368.
Review
For citations:
Kunder E.V., Volkova M.V., Generalov I.I., Nevinsky G.A. CLINICAL AND DIAGNOSTIC SIGNIFICANCE OF DEOXYRIBONUCLEASE ACTIVITY OF POLYCLONAL IgG AND BLOOD SERA IN PATIENTS WITH SPONDYLOARTHRITIS. Medical Immunology (Russia). 2012;14(4-5):337-346. (In Russ.) https://doi.org/10.15789/1563-0625-2012-4-5-337-346
Views:
778
Email this article 