PARTICIPATION OF TLR4 IN ENGULFMENT OF ESCHERICHIA COLI BY HUMAN BLOOD NEUTROPHILS IN PRESENCE OF LIPOPOLYSACCHARIDES
https://doi.org/10.15789/1563-0625-2012-3-219-222
Abstract
Abstract. TLR4 is a key player in signaling system of host cells. Possible role of TLR4 is actively discussed, e.g. its significance for phagocytosis. A capacity of neutrophils to engulf FITC-labeled E. coli bacteria upon activation with LPS of different origin was studied in presence of anti-TLR4 Mab’s (HTA125 clone). It was shown that, in whole blood, TLR4 does not play any essential role in engulfment of bacteria by the neutrophils. Phagocytic activity of neutrophils in blood increases increased after their priming with E. coli endotoxins. LPS from Rb. сapsulatus did not affect phagocytosis. In presence of endotoxins, the degree of TLR4 involvement in neutrophil phagocytosis depends on LPS structure.
About the Authors
S. V. Zubova
Институт фундаментальных проблем биологии РАН, г. Пущино, Московская область
Russian Federation
Russian Federation
S. V. Grachev
Московский государственный медицинский университет им. И.М. Сеченова, Москва
Russian Federation
Russian Federation
I. R. Prokhorenko
Институт фундаментальных проблем биологии РАН, г. Пущино, Московская область
Московский государственный медицинский университет им. И.М. Сеченова, Москва
Russian Federation
Russian Federation
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Review
For citations:
Zubova S.V., Grachev S.V., Prokhorenko I.R. PARTICIPATION OF TLR4 IN ENGULFMENT OF ESCHERICHIA COLI BY HUMAN BLOOD NEUTROPHILS IN PRESENCE OF LIPOPOLYSACCHARIDES. Medical Immunology (Russia). 2012;14(3):219-222. (In Russ.) https://doi.org/10.15789/1563-0625-2012-3-219-222
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