EXPRESSION OF T-bet FACTOR IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN BRONCHIAL ASTHMA
https://doi.org/10.15789/1563-0625-2012-1-2-169-173
Abstract
Abstract. We studied expression of transcription factor T-bet protein in allergic (ABA) versus non-allergic bronchial asthma (NABA), depending on severity grade and clinical phase of the disease. A Western blotting technique was applied, according to the standard protocol (Amersham), using polyclonal anti-T-bet antibodies at a 1:500 dilution (Santa Cruz Biotechnology, UK). The protein levels were quantified, using β-actin as a reference. It is shown for the first time that T-bet expression in ABA patients, as compared to healthy controls, was reduced with increasing disease severity, but did not correlate with clinical phase of the disease. In NABA patients, no sufficient differences in T-bet expression were revealed, as compared with healthy controls. The work was supported by St.Petersburg governmental grant PD04-4.0-102 (Certificate № ASP604079).
About the Authors
V. N. Mineev
Санкт-Петербургский государственный медицинский университет имени академика И.П. Павлова,
Санкт-Петербург
Russian Federation
Russian Federation
L. N. Sorokina
Санкт-Петербургский государственный медицинский университет имени академика И.П. Павлова,
Санкт-Петербург
Russian Federation
Russian Federation
References
1. Минеев В.Н., Сорокина Л.Н. Экспрессия STAT6 в лимфоцитах периферической крови больных аллергической бронхиальной астмой // Медицинская иммунология. – 2007. – Т. 9, № 4-5. – С. 405-411.
2. Минеев В.Н., Сорокина Л.Н., Нёма М.А. Влияние IL4 на активность транскрипционного фактора STAT6 в лимфоцитах периферической крови больных бронхиальной астмой // Медицинская иммунология. – 2009. – Т. 11, № 2-3. – С. 177-184.
3. Минеев В.Н., Сорокина, Л.Н., Нёма М.А., Трофимов В.И. Экспрессия транскрипционного фактора GATA-3 в лимфоцитах периферической крови больных бронхиальной астмой // Медицинская иммунология. – 2010. – Т. 12, № 1-2. – С. 21-28.
4. Фрейдлин И.С., Тотолян А.А. Иммунологические механизмы аллергических реакций // Общая аллергология. Т. 1 / Под ред. Г.Б. Федосеева. – СПб.: Нордмед-Издат, 2001. – С. 169-381.
5. Buono C., Binder C.J., Stavrakis G., Witztum J.L., Glimcher L.H., Lichtman A.H. T-bet deficiency reduces atherosclerosis and alters plaque antigen-specific immune responses // Proc. Natl. Acad. Sci. USA.– 2005. – Vol. 102 (5). – P. 1596-1601.
6. Finotto S., Neurath M.F., Glickman J.N., Qin S., Lehr H.A., Green F.H., Ackerman K., Haley K., Galle P.R., Szabo S.J., Drazen J.M., De Sanctis G.T., Glimcher L.H. Development of spontaneous airway changes consistent with human asthma in mice lacking T-bet // Science. – 2002. – Vol. 295. – P.336-338.
7. Ko F.W.S., Lun S.W.M., Wong C.K., Szeto C.C., Lam C.W.K., Leung T.F., Hui D.S.C. Decreased T-bet expression and changes in chemokine levels in adults with asthma // J. Clin. Exp. Immunol. – 2007. – Vol. 147. – P. 526-532.
8. Szabo S.J., Kim S.T., Costa G.L., Zhang X., Fathman C.G., Glimcher L.H. A novel transcription factor, T-bet, directs Th1 lineage commitment // Cell. – 2000. – Vol. 100. – P. 655-669.
Review
For citations:
Mineev V.N., Sorokina L.N. EXPRESSION OF T-bet FACTOR IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN BRONCHIAL ASTHMA. Medical Immunology (Russia). 2012;14(1-2):169-173. (In Russ.) https://doi.org/10.15789/1563-0625-2012-1-2-169-173
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