EVALUATION OF NEUTROPHIL GRANULOCYTE RESPIRATORY BURST IN COLORECTAL CANCER

Abstract

Colorectal cancer (CRC) is the third most common cancer in men and the second most common cancer in women. The ambiguity of neutrophils in the implementation of the immune response indicates their transformation into protumor cells in the late stages of the disease. In this regard, the aim of the study was to study the respiratory burst of neutrophilic granulocytes in the blood of patients with colorectal cancer at different stages of the disease and in the dynamics of treatment. The study included 99 patients with colorectal cancer in different stages and 112 practically healthy volunteers. The respiratory burst of neutrophils was assessed using chemiluminescent analysis of neutrophil granulocyte activity for 90 minutes on a 36-channel chemiluminescent analyzer “CL3607” (Russia). Statistical data processing was performed using Statistica 10 software packages (StatSoftInc, USA). Statistical significance of differences was determined using the Mann–Whitney criterion equal to p<0.05. In patients with CRC, an increase in the intensity of CL and total synthesis of ROS was recorded both in a state of relative rest and under additional functional load, reflecting the state of the respiratory burst in patients' neutrophils, both on the first day of admission and on the 7th day after surgery. The activation index is increased in patients with CRC at all stages and throughout the examination. Significant deviations in the indices of chemiluminescent activity of neutrophil granulocytes were recorded in patients with CRC: before surgery, an increase in the intensity of spontaneous CL was detected at stages II-IV of the disease, and induced CL at all stages, with the luminescence intensity at the late stage being higher. After surgical treatment, the spontaneous intensity of ROS production at stages I-III normalized, in the induced test, NG patients were still more reactive than the controls. The total ROS production by NG patients with CRC at all stages exceeded the indices of practically healthy people in the spontaneous and induced CL test both upon admission to the hospital and after treatment. The existing reserve capacity for ROS synthesis is also indicated by the increased neutrophil granulocyte activation index in all groups.

1. Порядковый номер ссылки Авторы, название публикации и источника, где она опубликована, выходные данные ФИО, название публикации и источника на английском Полный интернет-адрес (URL) цитируемой статьи или ее doi.

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