EXPRESSION OF ERGOTOPE-ASSOCIATED MARKERS BY ACTIVATED T-LYMPHOCYTES
https://doi.org/10.15789/1563-0625-2009-2-3-256-260
Abstract
Abstract. Anti-ergotypic response is considered to contribute significantly to the regulation of immune response. Ergotope-associated antigenic determinants include molecules upregulated on the surface of activated T cells (CD25, hsp60 and others). Anti-ergotypic cells are directed against these surface determinants usually complexed to MHC-I and/or MHC-II. Here we demonstrate regulated expression of HLA-Dr, СD25, hsp60 and hTERT mRNA during in vitro activation of peripheral T-lymphocytes with anti-CD3 antibodies and IL - 2, with reactivation on day 7. The percentage of CD25-expressing T cells showed sharp increase as early, as by the day 3 of activation, and it varied only slightly at later terms. The hsp60 levels rose as well, reached a peak at day 3, and gradually decreased thereafter. HLA-Dr expression was induced upon the activation, with a peak at the days 8 or 10. hTERT mRNA amounts also increased, as compared with baseline values, showing two peaks at the days 3 and 8. Anti-ergotopic response may control autoimmunity by targeting the activated T cells, regardless of their specificity. Therefore, immunotherapy of different autoimmune disorders aiming to activate anti-ergotopic responses, may be of a sufficient clinical interest.
About the Authors
O. Yu. Korol’kova
ГУ НИИ клинической иммунологии СО РАМН, г. Новосибирск
Russian Federation
Russian Federation
V. V. Senyukov
ГУ НИИ клинической иммунологии СО РАМН, г. Новосибирск
Russian Federation
Russian Federation
V. S. Kozhevnikov
ГУ НИИ клинической иммунологии СО РАМН, г. Новосибирск
Russian Federation
Russian Federation
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Review
For citations:
Korol’kova O.Yu., Senyukov V.V., Kozhevnikov V.S. EXPRESSION OF ERGOTOPE-ASSOCIATED MARKERS BY ACTIVATED T-LYMPHOCYTES. Medical Immunology (Russia). 2009;11(2-3):256-260. (In Russ.) https://doi.org/10.15789/1563-0625-2009-2-3-256-260
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