LEVEL SCF DURING PHYSICAL ACTIVITIES OF VARIOUS INTENSITIES AND AFTER ADMINISTRATION OF MELDONIUM

Abstract

Regenerative mechanisms in the liver during damage are associated with the activation of cytokines. SCF is an important signaling system; in addition to regulating the proliferation of hematopoietic stem cells, SCF is involved in liver restoration and regeneration after damage. In this regard, the aim of the study was to study the level of SCF in blood plasma and liver during physical activity of varying intensity and after taking meldonium. Material and methods of study. The experiments were conducted on male rats. The experiments were conducted in two stages. At the first stage, the rats were given loads of varying intensity: the 1st group of rats performed light physical activity, the 2nd group - moderate, the 3rd group performed heavy physical activity. A total of 10 sessions of water loading were performed. Intact animals were male rats of sexually mature age, which did not undergo physical activity, the number of animals corresponded to the number of experimental ones. The concentration of SCF (Rat Stem cell factor/mast cell growth factor) in blood plasma was determined using enzyme immunoassay, the level of SCF in the liver was studied using enzyme immunoassay. The study revealed that in the intact group, the SCF level was higher in the liver tissue than in the blood plasma. In the first group of animals, an increase in SCF by 1.5 times in the blood and a decrease in the liver were noted compared to the intact group (p < 0.05). In the second group, the SCF indicator in the liver increased, and in the blood there was a decrease by 1.1 times compared to the first group, but the indicator remained higher than in intact animals by 1.4 times (p < 0.05). In the third group, a decrease in SCF was noted both in the blood and in the liver compared to intact animals (p < 0.05). After the use of meldonium during mild and moderate physical exertion, an increase in SCF in the liver by 1.1 times was noted, during severe physical exertion by 1.4 times compared to the experimental animals that did not receive meldonium. Thus, taking meldonium during physical exercise of varying intensity led to a significant increase in the concentration of SCF in liver tissue.

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