ANTI-TUMOR ACTIVITY OF DENDRITIC CELLS IN HEALTHY DONORS AND PATIENTS WITH BRAIN TUMORS
https://doi.org/10.15789/1563-0625-2010-3-199-206
Abstract
In present work, a comparative analysis of cytostatic and cytotoxic activity of IL-4 and IFNα-induced dendritic cells (IL-4- and IFN-DCs) has been performed in healthy donors and patients with brain tumors. Cytostatic activity was evaluated, as a capacity of DCs to inhibit growth of NK-resistant HEp-2 and A-549 tumor cell lines, whereas cytotoxic activity was measured by assessment of HEp-2 lysis. As compared with IL4-DC, IFN-DCs from healthy donors displayed a more pronounced cytostatic effect and similar cytotoxic activity against HEp-2 cells. In the patients with brain tumors, IFN-DCs were characterized by decreased cytotoxic activity. Meanwhile, DCs from the patients with malignant gliomas virtually did not exhibit a cytotoxic potential, whereas IFN-DCs from the patients with benign brain tumors retained their cytotoxic properties. A comparative study has shown that, in contrast to healthy donors, IFN-DCs from the patients with either malignant or benign brain tumors did not inhibit HEp-2 proliferation. The loss of DC-mediated cytostatic activity was not associated with decrease of CD123+ DCs (known as cells with maximal tumor-inhibitory potential), and, most likely, it resulted from functional changes in IFN-DC populations.
About the Authors
E. R. Chernykh
НИИ клинической иммунологии СО РАМН, г. Новосибирск
Russian Federation
Russian Federation
O. Yu. Leplina
НИИ клинической иммунологии СО РАМН, г. Новосибирск
Russian Federation
Russian Federation
T. V. Tyrinova
НИИ клинической иммунологии СО РАМН, г. Новосибирск
Russian Federation
Russian Federation
M. A. Tikhonova
НИИ клинической иммунологии СО РАМН, г. Новосибирск
Russian Federation
Russian Federation
V. V. Stupak
ФГУ НИИ травматологии и ортопедии Росмедтехнологий, г. Новосибирск
Russian Federation
Russian Federation
S. V. Mishinov
ФГУ НИИ травматологии и ортопедии Росмедтехнологий, г. Новосибирск
Russian Federation
Russian Federation
I. V. Pendyurin
ФГУ НИИ травматологии и ортопедии Росмедтехнологий, г. Новосибирск
Russian Federation
Russian Federation
A. A. Ostanin
НИИ клинической иммунологии СО РАМН, г. Новосибирск
Russian Federation
Russian Federation
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Review
For citations:
Chernykh E.R., Leplina O.Yu., Tyrinova T.V., Tikhonova M.A., Stupak V.V., Mishinov S.V., Pendyurin I.V., Ostanin A.A. ANTI-TUMOR ACTIVITY OF DENDRITIC CELLS IN HEALTHY DONORS AND PATIENTS WITH BRAIN TUMORS. Medical Immunology (Russia). 2010;12(3):199-206. (In Russ.) https://doi.org/10.15789/1563-0625-2010-3-199-206
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