СHANGES OF TLR GENE EXPRESSION IN CERVICAL EPITHELIUM FROM THE PATIENTS WITH UROGENITAL INFLAMMATORY DISEASES
https://doi.org/10.15789/1563-0625-2015-3-269-274
Abstract
Toll-like receptors (TLR) are involved into innate immune recognition of microorganisms, including pathogenic bacteria. The aim of this work is to assess relative levels of TLR2 and TLR9 expression in cervical epithelial cells of women with inflammatory diseases of pelvic organs. The total group of patients consisted of 47 persons, including 20 women with urogenital infections, and 27 women comprised a control group. Using real-time PCR, we identified the opportunistic pathogens and gene expression levels of TLR2, TLR9 in the cervix epithelial cells. It was shown that expression of TLR9 in the group with urogenital infections was 13.7-fold higher than in control group. We have revealed that a significant increase of TLR9 expression in the mucosal epithelium from cervical canal correlated with detection of infectious pathogens in the samples.
About the Authors
O. A. Svitich
I.I. Mechnikov Scientific Research Institute of Vaccines and Serums, Moscow
Russian Federation
Russian Federation
PhD, MD (Medicine), Head, Laboratory of Molecular Immunology/
Address for correspondence: Svitich Oksana A. I.I. Mechnikov Scientific Research Institute of Vaccines and Serums 690062,Russian Federation,Moscow,Zoologicheskii Lane, 8, 100. Phone: 7 (926) 148-83-22. Fax: 7 (495) 674-57-10. E-mail: svitichoa@yandex.ru
L. I. Krasnoproshina
I.I. Mechnikov Scientific Research Institute of Vaccines and Serums, Moscow
Russian Federation
PhD, MD (Medicine), Professor, Chief, Laboratory of Immunological Methods
Russian Federation
PhD, MD (Medicine), Professor, Chief, Laboratory of Immunological Methods
L. V. Gankovskaya
Russian National N.I. Pyrogov Research Medical University, Moscow
Russian Federation
PhD, MD (Medicine), Professor, Head, Department of Immunology
Russian Federation
PhD, MD (Medicine), Professor, Head, Department of Immunology
L. V. Shibina
I.I. Mechnikov Scientific Research Institute of Vaccines and Serums, Moscow
Russian Federation
PhD (Medicine), Laboratory of Immunological Methods
Russian Federation
PhD (Medicine), Laboratory of Immunological Methods
I. I. Zaitseva
Russian National N.I. Pyrogov Research Medical University, Moscow
Russian Federation
Student Engaged on Degree Thesis, Department of Immunology
Russian Federation
Student Engaged on Degree Thesis, Department of Immunology
References
1. Савельева Г.М., Бреусенко В.Г. Гинекология. М.: ГЭОТАР-Медиа, 2007. 432 с. [Savelieva G.M., Breusenko V.G. Gynaecology]. Moscow: GEOTAR-Media, 2007. 432 p.
2. Ковальчук Л.В., Макаров О.В., Ганковская Л.В. Невынашивание беременности, инфекция, врожденный иммунитет. М.: ГЭОТАР-Медиа, 2007. 176 с. [Kovalchuk L.V., Makarov O.V., Gankovskaya L.V. Preterm birth, infection, innate immunity]. Moscow: GEOTAR-Media, 2007. 176 p.
3. Ковальчук Л.В., Ганковская Л.В., Свитич О.А., Мироншиченкова А.М., Ганковский В.А. Роль TOLLподобных рецепторов в патогенезе инфекционных заболеваний человека // Курский научно-практический вестник «Человек и его здоровье», 2012. № 2. С. 147-153. [Kovalchuk L.V., Gankovskaya L.V. Svitich O.A., Miroshnichrnkova A.M., Gankovskii V.A. The role of TOLL-like receptors in pathogenesis of infection humon deseases]. Kurskij nauchno-prakticheskij vestnik “Chelovek i ego zdorov’e” = Kursk Scientific and Practical Journal «Human and Health», 2012, no. 2, pp. 147-153. (In Russ.)]
4. Ганковская О.А., Ганковская Л.В., Сомова О.Ю., Зверев В.В. Toll-подобные рецепторы, распознающие лиганды вируса герпеса // Журнал микробиологии, эпидемиологии и иммунобиологии, 2009. № 2. С. 108-111. [Gankovskaya O.A., Gankovskaya L.V., Somova O.Yu., Zverev V.V. Toll-like receptors recognizing ligands of herpesvirus]. Zhurnal mikrobiologii, epidemiologii i immunobiologii = Journal of Microbiology, Epidemiology, Immunobiology, 2009, no. 2, pp. 108-111. (In Russ.)]
5. Ганковская О.А., Бахарева И.В., Ганковская Л.В., Сомова О.Ю., Зверев В.В. Исследование экспрессии генов TLR9, NF-kB, ФНОα в клетках слизистой цервикального канала беременных с герпесвирусной инфекцией // Журнал микробиологии, эпидемиологии и иммунобиологии, 2009. № 2. С.61-65. [Gankovskaya O.A., Bakhareva I.V., Gankovskaya L.V., Somova O.Yu., Zverev V.V. Study of expression of TLR9, NF-kappaB, TNFalpha genes in cells of cervical canal mucosa in pregnant women with herpesvirus infection]. Zhurnal mikrobiologii, epidemiologii i immunobiologii = Journal of Microbiology, Epidemiology, Immunobiology, 2009, no. 2, pp. 61-64. (In Russ.)]
6. Ганковская О.А., Зверев В.В., Лавров В.Ф., Блинкова Л.П., Ганковская Л.В., Кузнецов П.А. Изменение уровня экспрессии сигнальных рецепторов врожденного иммунитета при инфекции, вызванной Candida albicans in vitro и in vivo // Журнал микробиологии, эпидемиологии и иммунобиологии, 2009. № 3. С. 60-64. [Gankovskaya O.A., Zverev V.V., Lavrov V.F., Blinkova L.P., Gankovskaya L.V., Kuznetsov P.A. Changes of expression levels of innate immunity signaling receptors during Candida albicans infections in vitro and in vivo]. Zhurnal mikrobiologii, epidemiologii i immunobiologii = Journal of Microbiology, Epidemiology, Immunobiology, 2009, no. 3, pp. 60-63. (In Russ.)]
7. Гланц С. Медико-биологическая статистика. М.: Практика, 1998. 459 с. [Glants C. Medico-biological statistics]. Moscow: Praktika, 1998. 459 p.
Review
For citations:
Svitich O.A., Krasnoproshina L.I., Gankovskaya L.V., Shibina L.V., Zaitseva I.I. СHANGES OF TLR GENE EXPRESSION IN CERVICAL EPITHELIUM FROM THE PATIENTS WITH UROGENITAL INFLAMMATORY DISEASES. Medical Immunology (Russia). 2015;17(3):269-274. (In Russ.) https://doi.org/10.15789/1563-0625-2015-3-269-274
Views:
1150
Email this article 