CLINICAL SIGNIFICANCE OF Toll2, Toll4, CD14, AND HLA-DR EXPRESSION ON THE MONOCYTES IN PATIENTS WITH SEPSIS
https://doi.org/10.15789/1563-0625-2015-3-221-228
Abstract
Expression of Toll-like receptors (TLRs) on myeloid cells is of key importance for induction of systemic reactions caused by bacterial damage, and activation mechanisms of innate immune response. The study included thirty-six patients with sepsis in whom we assessed a prognostic value of TLR2, TLR4, CD14, HLA-DR expressed on monocytes, depending on severity of the disease and clinical outcomes. There were neither significant differences in TLR4 expression at all stages of clinical observation, nor correlations between expression of this marker on monocytes and severity of the disease, or outcomes of septic conditions. Low levels of MFI on monocytes TLR2, HLA-DR at 5 and 10 days, like as decreased expression of CD14 on 10th day of the disease were shown to be predictive for poor clinical outcomes of sepsis.
About the Authors
V. A. Lazanovich
Pacific State Medical University, Vladivostok
Russian Federation
Russian Federation
PhD (Medicine), Assistant Professor, Department of Clinical Laboratory Diagnostics, General and Clinical Immunology.
Address for correspondence: Lazanovich Vladimir A. G.P. Somov Research Institute of Epidemiology and Microbiology, Siberian Branch of Russian Academy of Medical Sciences 690062,Russian Federation,Vladivostok, The stoletiya pr., 14-49. Phone: 7 (914) 703-45-09. E-mail: immuno2003@mail.ru
E. V. Markelova
Pacific State Medical University, Vladivostok
Russian Federation
PhD, MD (Medicine), Professor, Head, Department of Human Physiology
Russian Federation
PhD, MD (Medicine), Professor, Head, Department of Human Physiology
G. A. Smirnov
Pacific State Medical University, Vladivostok
Russian Federation
PhD (Medicine), Associate Professor, Department of Intensive Care, Anesthesiology, and Emergency Medicine
Russian Federation
PhD (Medicine), Associate Professor, Department of Intensive Care, Anesthesiology, and Emergency Medicine
T. P. Smolina
G.P. Somov Research Institute of Epidemiology and Microbiology, Siberian Branch of Russian Academy of Medical Sciences, Vladivostok
Russian Federation
PhD (Medicine), Leading Research Associate, Laboratory of Immunology
Russian Federation
PhD (Medicine), Leading Research Associate, Laboratory of Immunology
References
1. Киселева Е.П. Новые представления о противоинфекционном иммунитете // Инфекция и иммунитет, 2011. Т. 1, № 1. C. 9-14. [Kisseleva E.P. New aspects of anti-infection immunity]. Infekcii i immunitet = Russian Journal of Infections and Immunity, 2011, Vol. 1, no. 1, рр. 9-14. (In Russ.)]
2. Лазанович В.А., Степанюк В.Н., Смирнов Г.А. Маркелова Е.В., Биомаркеры воспаления в прогнозе исхода абдоминального сепсиса // Фундаментальные исследования, 2012. № 4. С. 311-315. [Lazanovich V.A., Stepanyuk V.N., Smirnov G.A., Markelova E.V. Biomarkers inflammation in the forecast of the outcome of the abdominal sepsis]. Fundamental`nye issledovaniya = Fundamental Research, 2012, no. 4, рр. 311-315. (In Russ.)]
3. Чикилева И.О., Караулов А.В., Анисимова Н.Ю., Киселевский М.В. Двойственная роль толлподобных рецепторов в регуляции противоопухолевого иммунитета // Иммунология, 2010. № 1. С. 52-55. [Chikileva I.O., Karaulov A.V., Anisimova N.Yu., Kiselevskiy M.V. Dual role TLRs in regulation antitumor immunity]. Immunologiya = Immunology, 2010, no. 1, рр. 52-55. (In Russ.)]
4. Armstrong L., Medford A.R., Hunter K.J. Differential expression of Toll-like receptor (TLR)-2 and TLR-4 on monocytes in human sepsis. Clin. Exp. Immunol., 2004, Vol. 136, рр. 312-319.
5. Brunialti M.K., Martins P.S., Barbosa de Carvalho H. TLR2, TLR4, CD14, CD11B, and CD11C expressions on monocytes surface and cytokine production in patientswith sepsis, severe sepsis, and septic shock. Shock, 2006, Vol. 25, no. 4, рр. 351-357.
6. Dessing M.C., Schouten M., Draing C. Role played by Toll-like receptors 2 and 4 in lipoteichoic acidinduced lung inflammation and coagulation. Infect. Dis., 2008, Vol. 197, no. 2, рр. 245-252.
7. Gaieski D.F., Edwards J.M., Kallan M.J. Benchmarking the incidence and mortality of severe sepsis in the United States. Crit. Care Med., 2013, Vol 41, no. 5, рр. 1167-1174.
8. Hiki N., Berger D., Prigl C. Endotoxin binding and elimination by monocytes: secretionof soluble CD14 represents an inducible mechanism counteracting reduced expression of membrane CD14 in patients with sepsis and in a patient with paroxysmal nocturnal emoglobinuria. Infect. Immun., 1998, Vol. 66, no. 3, pp. 1135-1141.
9. Kawai T., Akira S. The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors. Nat. Immunol., 2010, Vol. 11, no. 5, рр. 373-384.
10. Lukaszewicz A.C., Faivre V., Payen D. Is monocyte HLA-DR expression monitoring a useful tool to predict the risk of secondary infection? Minerva Anestesiol., 2010, Vol. 76 no. 9, рр. 737-743.
11. Moseley P. Stress proteins and the immune response. Immunopharmacology, 2000, Vol. 48, no. 3, рр. 299302.
12. Opal S.M., Laterre P.F., Francois B. ACCESS Study Group Effect of eritoran, an randomized trial. JAMA, 2013, Vol. 309, рр. 1154-1162.
13. Gaieski D.F., Edwards J.M., Kallan M.J. Benchmarking the incidence and mortality of severe sepsis in the United States. Crit. Care Med., 2013, Vol. 41, no. 5, рр. 1167-1174.
14. Ranieri V.M., Thompson B.T., Barie P.S. Drotrecogin alfa (activated) in adults with septic shock. PROWESSSHOCK Study Group. N. Engl. J. Med, 2012, Vol. 366, no. 22, рр. 2055-2064.
15. Schaaf B., Luitjens K., Goldmann T., van Bremen T., Sayk F., Dodt C., Dalhoff K., Droemann D. Mortality in human sepsis is associated with downregulation of Toll -like receptor 2 and CD14 expression on blood monocytes. Diagn. Pathol., 2009, no. 16, рр. 4-12.
16. Tsung A., Sahai R., Tanaka H.The nuclear factor HMGB1 mediates hepatic injury after murine liver ischemia-reperfusion. J. Exp. Med., 2005, Vol. 201, no. 7, рр. 1135-1143.
17. Wittebole X., Castanares-Zapatero D., Laterre P.F. Toll-like receptor 4 modulation as a strategy to treat sepsis. Mediators Inflamm., 2010, Vol. 2010, рр. 5683-5696.
18. Xu J., Zhang X., Pelayo R. Extracellular histones are major mediators of death in sepsis. Nat. Med., 2009, Vol. 15, no. 11, рр.1318-1321.
Review
For citations:
Lazanovich V.A., Markelova E.V., Smirnov G.A., Smolina T.P. CLINICAL SIGNIFICANCE OF Toll2, Toll4, CD14, AND HLA-DR EXPRESSION ON THE MONOCYTES IN PATIENTS WITH SEPSIS. Medical Immunology (Russia). 2015;17(3):221-228. (In Russ.) https://doi.org/10.15789/1563-0625-2015-3-221-228
Views:
1281
Email this article 