СHANGES IN PARAMETERS OF LUMINOL-DEPENDENT AND LUCIGENIN-DEPENDENT CHEMILUMINESCENCE OF PERIPHERAL BLOOD NEUTROPHILS IN PATIENTS WITH BLADDER CANCER IN THE DISEASE DYNAMICS
https://doi.org/10.15789/1563-0625-2015-2-173-178
Abstract
The study deals with parameters of luminol-dependent and lucigenin-dependent chemiluminescence (CL) of peripheral blood neutrophils from patients with bladder cancer (BC) prior to surgical treatment.
We examined sixty patients (45 to 55 years old) with advanced bladder cancer (TNM) prior to the operation, and forty-six patients at 10 days after surgical treatment. A control group consisted of 56 healthy donors. Luminol-dependent and lucigenin-dependent chemiluminescence of blood neutrophils was assessed according to De Sole et al. (1983).
Chemiluminescence assays of peripheral blood neutrophils from the patients with bladder cancer revealed changes in production of reactive oxygen species (ROS), both for initial stage of oxidation reaction, and total level of active oxygen radicals. We have found disturbed values of primary-to-secondary ROS ratio in the cells.
In the patients with bladder cancer, some changes in oxidative metabolism of the blood neutrophils have been registered. These alterations may play an important role in promotion of potential effector cell functions, thus, probably, affecting the whole-scale development of a cytopathic effect exerted by neutrophilic granulocytes.
About the Authors
L. M. Kurtasova
Krasnoyarsk State Medical University named after Professor V.F. Voyno-Yasenetsky of the Ministry of Health Care of the Russian Federation
660022, Russian Federation, Krasnoyarsk, Partizan Zheleznyak str., 1.
Russian Federation
Russian Federation
PhD, MD (Medicine), Professor, Department of Clinical Immunology, Krasnoyarsk State Medical University named after Professor V.F. Voyno-Yasenetsky of the Ministry of Health Care of the Russian Federation, Krasnoyarsk, Russian Federation
E. V. Semenov
Krasnoyarsk State Medical University named after Professor V.F. Voyno-Yasenetsky of the Ministry of Health Care of the Russian Federation
660022, Russian Federation, Krasnoyarsk, Partizan Zheleznyak str., 1.
Russian Federation
Russian Federation
PhD Candidate, Department of Oncology and Radiotherapy with Course of Postgraduate Education, Krasnoyarsk State Medical University named after Professor V.F. Voyno-Yasenetsky of the Ministry of Health Care of the Russian Federation, Krasnoyarsk, Russian Federation
R. A. Zukov
Krasnoyarsk State Medical University named after Professor V.F. Voyno-Yasenetsky of the Ministry of Health Care of the Russian Federation
660022, Russian Federation, Krasnoyarsk, Partizan Zheleznyak str., 1.
Russian Federation
Russian Federation
PhD (Medicine), Associate Professor, Chief, Department of Oncology and Radiotherapy with Course of Postgraduate Education, Krasnoyarsk State Medical University named after Professor V.F. Voyno-Yasenetsky of the Ministry of Health Care of the Russian Federation, Krasnoyarsk, Russian Federation
E. A. Shkapova
Krasnoyarsk Regional Clinical Oncology Center named after A.I. Kryzhanovsky, Krasnoyarsk, Russian Federation
Russian Federation
Russian Federation
PhD (Biology), Doctor in Clinical Laboratory Diagnostics, Krasnoyarsk Regional Clinical Oncology Center named after A.I. Kryzhanovsky, Krasnoyarsk, Russian Federation
References
1. Владимиров Ю.А., Проскурина Е.В. Свободные радикалы и клеточная хемилюминесценция // Успехи биологической химии, 2009. Т. 49. С. 341-388. [Vladimirov Yu.A., Proskurina E.V. Free radicals and cellular chemiluminescence. Uspekhi biologicheskoy khimii = Advances Biological Chemistry, 2009, Vol. 49, pp. 341-388. [In Russ.])
2. Коленчукова О.А., Савченко А.А., Смирнова С.В. Особенности люминоли люцигенин-зависимой хемилюминесценции нейтрофильных гранулоцитов у больных хроническим риносинуситом // Медицинская иммунология, 2010. Т. 12, No 4-5. С. 437-440. [Kolenchukova O.A., Savchenko A.A., Smirnova S.V. Features of luminoland lucigenin-dependent chemiluminescence of neutrophilic granulocytes in patients with chronic rhinosinusitis. Meditsinskaya Immunologiya = Medical Immunology (Russia), 2010, Vol. 12, no. 4-5, pp. 437-440. [In Russ.])
3. Нестерова И.В., Ковалева С.В., Чудилова Г.А., Ломтатидзе Л.В., Евглевский А.А. Двойственная роль нейтрофильных гранулоцитов в реализации противоопухолевой защиты // Иммунология, 2012. No 5. С. 281-287. [Nesterova I.V., Kovaleva S.V., Chudilova G.A., Lomtatidze L.V., Yevglevsky A.A. The dual role of neutrophilic granulocytes in the realization anti-tumor protection. Immunologiya = Immunology, 2012, no. 5, pp. 281287. [In Russ.])
4. Benbarek H., Ayad A., Deby-Dupont G., Boukraa L., Serteyn D. Modulatory effects of non-steroidal antiinflammatory drugs on the luminol and lucigenin amplified chemiluminescence of equine neutrophils. Vet. Res. Commun., 2012, Vol. 36, no. 1, pp. 29-33.
5. De Sole P., Lippa S., Lixxarru G. Whole blood chemiluminescence: a new technical approach to access oxyden-dependent microbial activity of granulocytes. J. Clin. Lab. Autom., 1983, Vol. 3, pp. 391-400.
6. Di Carbo E., Forni G., Musiani P. Neutrophils in the antitumoral immune response. Chem. Immunol. Allergy, 2003, Vol. 83, pp. 182-286.
7. Kubota K., Saiwai H., Kumamaru H., Maeda T., Ohkawa Y., Aratani Y., Nagano T., Iwamoto Y., Okada S. Myeloperoxidase exacerbates secondary injury by generating highly reactive oxygen species and mediating neutrophil recruitment in experimental spinal cord injury. Spine, 2012, Vol. 37, no. 16, pp. 1363-1369.
8. Marcoux J., Man P., Petit-Haertlein I., Vives C., Forest E., Fieschi F. P47phox molecular activation for assembly of the neutrophil NADPH oxidase complex. J. Biol. Chem., 2010, Vol. 285, no. 37, pp. 28980-28990.
9. Seth R., Ribeiro M., Romaschin A., Scott J.A., Manno M., Scott J.A., Liss G.M., Tarlo S.M. Occupational endotoxin exposure and a novel luminol-enhanced chemiluminescence assay of nasal lavage neutrophil activation. J. Allergy Clin. Immunol., 2011, Vol. 127, no. 1, pp. 272-275.
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For citations:
Kurtasova L.M., Semenov E.V., Zukov R.A., Shkapova E.A. СHANGES IN PARAMETERS OF LUMINOL-DEPENDENT AND LUCIGENIN-DEPENDENT CHEMILUMINESCENCE OF PERIPHERAL BLOOD NEUTROPHILS IN PATIENTS WITH BLADDER CANCER IN THE DISEASE DYNAMICS. Medical Immunology (Russia). 2015;17(2):173-178. (In Russ.) https://doi.org/10.15789/1563-0625-2015-2-173-178
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