LIGAND INDUCED CHEMOTAXIS OF MACROPHAGE CELL LINE U937
https://doi.org/10.15789/1563-0625-2014-5-443-448
Abstract
Treatment of malignant tumors is among challenging issues of clinical medicine. Dissemination and matestasis of tumor cells plays a major role in oncology, being immediately dependent on chemotaxis of tumor cells. Hence, the aim of our study is to evaluate migration of tumor cells, in terms of ligand-mediated chemotaxis of a human U 937 lymphoma cell line. Synthetic TLR ligands (DNA_lig, RNA_lig) were used as chemoattractants. Assessment of time-dependent TLR expression by the migrating cells (including TLR2, TLR3, TLR7, TLR9) was carried out by means of real-time PCR, using Boyden’s chamber system for the migration assays. We have revealed an increased cell migration towards DNA_ lig and TNFα gradient. Expression of TLR2, TLR7 and TLR9 was found to be increased under the influence of TNFα (respectively 1.5-, 4- and 19-fold). Under the influence of DNA_lig, TLR9 expression was 105-fold increased, whereas TLR3 expression was 2-fold higher, along with decrease of TLR2 expression. Expression of TLR 3 was shown to be 3-times higher under the influence of RNA_lig. The effects observed could be potentially applied for suppression of tumor cell chemotaxis by means of these ligands and by influencing different pathways of
chemotaxis regulation.
About the Authors
A. B. FilinaRussian Federation
Laboratory Assistant, Laboratory of Molecular Immunology, I.I. Mechnikov Research Institute of Vaccines and Serology, Russian Academy of Medical Sciences, Moscow, Russian Federation
O. A. Svitich
Russian Federation
PhD, MD, Chief, Laboratory of Molecular Immunology, I.I. Mechnikov Research Institute of Vaccines and Serology 105064, Russian Federation, Moscow, Maly Kazenny Lane, 5а. Phone: 7 (926) 148-83-22
L. V. Gankovskaya
Russian Federation
PhD, MD (Medicine), Chief, Department of Immunology, N.I. Pirogov Russian State Medical University, Russian Ministry of Health Care, Moscow, Russian Federation
P. A. Labzhinov
Russian Federation
PhD Candidate, Laboratory of Molecular Immunology, I.I. Mechnikov Research Institute of Vaccines and Serology, Russian Academy of Medical Sciences, Moscow, Russian Federation
T. M. Parfenova
Russian Federation
Senior Research Associate, Laboratory of Viral Diagnostics, I.I. Mechnikov Research Institute of Vaccines and Serology, Russian Academy of Medical Sciences, Moscow, Russian Federation
V. V. Zverev
Russian Federation
PhD, MD (Biology), Professor, Full Member of RAS, Director, I.I. Mechnikov Research Institute of Vaccines and Serology, Russian Academy of Medical Sciences, Moscow, Russian Federation
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Review
For citations:
Filina A.B., Svitich O.A., Gankovskaya L.V., Labzhinov P.A., Parfenova T.M., Zverev V.V. LIGAND INDUCED CHEMOTAXIS OF MACROPHAGE CELL LINE U937. Medical Immunology (Russia). 2014;16(5):443-448. https://doi.org/10.15789/1563-0625-2014-5-443-448
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