EXPRESSION OF A NEW A3 ANTIGEN IN THE CELLS OF PATIENTS WITH VARIOUS LYMPHOPROLIFERATIVE DISEASES
https://doi.org/10.15789/1563-0625-2014-5-437-442
Abstract
We have conducted a study of a novel monoclonal A3 antibody raised by means of hybridoma biotechnology. The study was performed with malignant cells of the patients with various lymphoproliferative disorders, and persons with nonmalignant diseases, as compared with intact lymphocytes from healthy people,
using a method of immunocytochemical staining and indirect immunofluorescence technique. It was found that in cases of lymphoproliferative diseases with low proliferation rates, as based on the numbers of Ki-67 positive cells, as well as in non-malignant blood diseases, the A3 antigen was localized in nucleoli, and it was visualized as focal fluorescence. In malignant lymphoproliferative diseases with high proliferation indexes, the number of brightly fluorescent foci is observed, with formation of necklace-like structures within the nucleolar structures. The obtained data point to a diagnostic significance of A3 Mab in assessment of cellular proliferative rates in patients with various lymphoproliferative diseases. It was established that, in contrast to Ki-67, the proliferation stage could be determined for each cell, according to the number of fuorescent foci in nucleoli. This specific property of the A3 antigen points to its significance for diagnostics and malignancy staging of lymphoproliferative disorders.
About the Authors
N. L. Deineko
National Research Center for Hematology, Moscow, Russian Federation
Russian Federation
Russian Federation
PhD (Biology), Research Associate, Clinical Laboratory of Transfusion Immunology and Pathology Department, National Research Center for Hematology Moscow, Russian Federation
T. I. Bulycheva
National Research Center for Hematology, Moscow, Russian Federation
Russian Federation
Russian Federation
PhD, MD, Professor, Research Consultant, Clinical Laboratory of Transfusion Immunology and Pathology Department, National Research Center for Hematology 125167, Russian Federation, Moscow, Novozykovsky pass., 4. Phone: 7 (495) 613-26-56
A. M. Kovrigina
National Research Center for Hematology, Moscow, Russian Federation
Russian Federation
Russian Federation
PhD, MD (Biology), Professor, Head, Pathology Department, National Research Center for Hematology Moscow, Russian Federation
A. A. Grigoriyv
National Research Center for Hematology, Moscow, Russian Federation
Russian Federation
Russian Federation
PhD (Biology), Research Associate, Clinical Laboratory of Transfusion Immunology and Pathology Department, National Research Center for Hematology Moscow, Russian Federation
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Review
For citations:
Deineko N.L., Bulycheva T.I., Kovrigina A.M., Grigoriyv A.A. EXPRESSION OF A NEW A3 ANTIGEN IN THE CELLS OF PATIENTS WITH VARIOUS LYMPHOPROLIFERATIVE DISEASES. Medical Immunology (Russia). 2014;16(5):437-442. (In Russ.) https://doi.org/10.15789/1563-0625-2014-5-437-442
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