EXPANSION OF OPPORTUNITIES OF THE FLOW CYTOMETRY METHOD FOR CLINICAL AND IMMUNOLOGYCAL PRACTICE
https://doi.org/10.15789/1563-0625-2008-1-5-12
Abstract
Abstract. The purpose of this article was to make a broad audience of experts familiar to new developments in the method of flow cytometry that have found applications in medical and biologic studies, in order of their further implementation into everyday practice. These applications include the following approcahes: detection of antigen-specific T-cells by using of tetramer technology, flow cytometric determination of cytokines in biological liquids, determination of in vitro sensitivity of basophilic granulocytes to allergen effects, detection of Th1 and Т-reg cells by their cell surface markers. When using multiparametric analysis, a multi-step gating and other new technologies, the flow cytometry technique allows of location and tracing the majority of processes occurring in development of immune response. When studying these dynamic events, we get an opportunity to react adequately to apropriate changes, and to develop new approaches to correct altered cellular activities, that they should determinate.
About the Authors
S. V. Khaidukov
Институт биоорганической химии им. акад. М.М. Шемякина и Ю.А. Овчинникова РАН, Москва
Russian Federation
Russian Federation
A. V. Zurochka
ГОУ ВПО Челябинская государственная медицинская академия Росздрава, г. Челябинск
Russian Federation
Russian Federation
References
1. Зуева Е.Е. Иммунофенотипирование в диагностике острых лейкозов // Российский Биомедицинский Журнал – 2003. – Том 4, СТ. 132 – С. 471-478.
2. Зурочка А.В., Биргер О.В., Бастрон А.С. Состояние иммунной системы у больных с комбинированными и сочетанными поражениями иммунной системы // Современные проблемы аллергологии, иммунологии и иммунофармакологии: Сб. тр. 5-го Конгресса РААКИ. – М., 2002. – С. 2-57.
3. Луговская С.А., Почтарь М.Е., Тупицын Н.Н. Иммунофенотипирование в диагностике гемобластозов. – М.-Тверь: ООО «Издательство Триада», 2005. – 168 с.
4. Хайдуков С.В., Литвинов И.С. Изменение гомеостаза ионов кальция в CD4+ Т-лимфоцитах периферической крови человека в процессе дифференцировки in vivo // Биохимия. – 2005. – Т. 70, № 6. – С. 838-849.
5. Altman J.D., Moss P.A., Goulder P.J., Barouch D.H., McHeyzer-Williams M.G., Bell J.I., McMichael A.J., Davis M.M. Phenotypic analysis of antigen-specific T lymphocytes // Science. – 1996. – Vol. 274. – P. 94-96.
6. Bishop J.E., Davis K.A. A flow cytometric immunoassay for b2-microglobulin in whole blood // J. Immunol. Meth. – 1997. – Vol. 210. – P. 79-87.
7. Boumiza R., Debard A.-L., Monneret G. The basophil activation test by flow cytometry: recent developments in clinical studies, standardization and emerging perspectives // Clin. Mol. Allergy. – 2005.– Vol. 3, N 9. – P. 1-8.
8. Buhring H.-J., Simmons P.J., Pudney M., Muller R., Jarrossay D., van Agthoven A., Willheim M., Brugger W., Valent P., Kanz L. The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multipotent and unipotent progenitors // Blood. – 1999. – Vol. 94, N 7. – P. 2343-2356.
9. Camilla C., Mely L., Magnan A., Casano B., Prato S., Debono S., Montero F., Defoort J.P., Martin M., Fert V. Flow cytometric microsphere-based immunoassay: analysis of secreted cytokines in whole-blood samples from asthmatics // Clin. Diagn. Lab. Immunol. – 2001. – Vol. 8, N 4. – P. 776-784.
10. Comans-Bitter W.M., de Groot R., van den Beemd R., Neijens H.J., Hop W.C., Groeneveld K., Hooijkaas H., van Dongen J.J. Immunophenotyping of blood lymphocytes in childhood. Reference values for lymphocyte subpopulations // J. Pediatr. – 1997. – Vol. 130, N 3. – P. 388-393.
11. Crucian B., Dunne P., Friedman H., Ragsdale R., Pross S., Widen R. Detection of altered T helper 1 and T helper 2 cytokine production by peripheral blood mononuclear cells in patients with multiple sclerosis utilizing intracellular cytokine detection by flow cytometry and surface marker analysis // Clin. Diagn. Lab. Immunol. – 1996. – Vol. 3, N 4. – P. 411-416.
12. Duramad P., McMahon C.W., Hubbard A., Eskenazi B., Holland N.T. Flow cytometric detection of intracellular Th1/Th2 cytokines using whole blood: validation of immunologic biomarker for use in epidemiologic studies // Cancer Epidemiol. Biomarkers Prev. – 2004. – Vol. 13, N 9. – P. 1452-1458.
13. Fritzsching B., Oberle N., Eberhardt N., Quick S., Haas J., Wildemann B., Krammer P.H., Suri-Payer E. In contrast to effector T cells, CD4+CD25+FoxP3+ regulatory T cells are highly susceptible to CD95 ligand – but not to TCR-mediated cell death // Immunology. – 2005. – Vol. 175, N 1. – P. 32-36.
14. Fulton R.J., McDade R.L., Smith P.L., Kienker L.J., Kettman J.R. Advanced multiplexed analysis with the FlowMetrics system // Clin. Chem. – 1997. – Vol. 43. – P. 1749-1756.
15. Harty J.T., Tvinnereim A.R., White D.W. CD8+ T cell effector mechanisms in resistance to infection // Annu Rev. Immunol. – 2000. – Vol. 18 – P. 275-230.
16. He X.H., Xu L.H., Liu Y. Procedure for preparing peptide-major histocompatibility complex tetramers for direct quantification of antigen-specific cytotoxic T lymphocytes // World J. Gastroenterol. – 2005. – Vol. 11. – P. 4180-4187.
17. He X.H., Zha Q.B., Liu Y., Xu L.H., Chi X.Y. High frequencies cytomegalovirus pp65(495-503)-specific CD8+ T cells in healthy young and elderly chinese donors: characterization of their phenotypes and TCRVβ usage // J. Clin. Immunol. – 2006. – Vol. 26. – P. 417-429.
18. Hsu S.C., Chen L.C., Kuo M.L., Huang J.L., Huang S.K. Novel SNPs in a candidate gene, CRTH2, for allergic diseases // Genes Immun. – 2002. – Vol. 3, N 2. – P. 114-116.
19. Ikinciogullari A., Kendirli T., Dogu F., Egin Y., Reisli I., Cin S., Babacan E. Peripheral blood lymphocyte subsets in healthy Turkish children // Turk. J. Pediatr. – 2004. – Vol. 46, N 2. – P. 125-130.
20. Iwasaki M., Nagata K., Takano S., Takahashi K., Ishii N., Ikezawa Z. Association of a new-type prostaglandin D2 receptor CRTH2 with circulating T helper 2 cells in patients with atopic dermatitis // J. Invest. Dermatol. – 2002. – Vol. 119, N 3. – P. 609-616.
21. Jaleco S., Swainson L., Dardalhon V., Burjanadze M., Kinet S., Taylor N. Homeostasis of naive and memory CD4+ T cells: IL-2 and IL-7 differentially regulate the balance between prolifera tion and Fasmediated apoptosis // J. Immunol. – 2003.– Vol. 171, N 1. – P. 61-68.
22. Kettman J.R., Davies T., Chandler D., Oliver K.G., Fulton R.J. Classification and properties of 64 multiplexed microsphere sets // Cytometry. – 1998. – Vol. 33. – P. 234-243.
23. Klebanoff C.A., Gattinoni L., Restifo N.P. CD8+ T-cell memory in tumor immunology and immunotherapy // Immunol. Rev. – 2006. – Vol. 211. – P. 214-224.
24. Lefranзois L., Marzo A. The descent of memory T-cell subsets // Annu Rev. Immunol. – 2006. – Vol. 6. – P. 618-623.
25. Lindmo T., Bormer O., Ungelstad J., Nustad K. Immunometric assay by flow cytometry using mixtures of two particle types of different affinity // J. Immunol. Methods. – 1990. – Vol. 126. – P. 183-189.
26. Lisi P.J., Huang C.W., Hoffman R.A., Teipel J.W. A fluorescence immunoassay for soluble antigens employing flow cytometric detection // Clin. Chem. Acta. – 1982. – Vol. 120. – P. 171-179.
27. Liu W., Putnam A.L., Xu-Yu Z., Szot G.L., Lee M.R., Zhu S., Gottlieb P.A., Kapranov P., Gingeras T.R., Fazekas de St. Groth B., Clayberger C., Soper D.M., Ziegler S.F., Bluestone J.A. CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ T reg cells // J. Exp. Med. – 2006.– Vol. 203, N 7. – P. 1701-1711.
28. Luider J., Cyfra M., Johnson P., Auer I. Impact of the new Beckman Coulter Cytomics FC 500 5-color flow cytometer on a regional flow Cytometry clinical laboratory servise // Laboratory Hematology. – 2004. – Vol. 10. – P. 102-108.
29. Maier R., Weger M., Haller-Schober E.M., ElShabrawi Y., Theisl A., Barth A., Aigner R., Haas A. Application of multiplex cytometric bead array technology for the measurement of angiogenic factors in the vitreous // Molecular Vision. – 2006. – Vol. 12. – P. 1143-1147.
30. Mason D.Y., Andre P., Bensussan A., Buckley C., Civin C., Clark E., de Haas M., Goyert S., Hadam M., Hart D., Horejsi V., Meuer S., Morissey J., SchwartzAlbiez R., Shaw S., Simmons D., Uguccioni M., van der Schoot E., Viver E., Zola H. CD antigens 2001 // Tissue Antigens. – 2001. – Vol. 58, N 6. – P. 425-430.
31. Meidenbauer N., Hoffmann T.K., Donnenbergc A.D. Direct visualization of antigen-specific T cells using peptide-MHC class I tetrameric complexes // Methods. – 2003. – Vol. 31. – P. 160-171.
32. Monneret G., Benoit Y., Debard A.-L., Gutowski M.C., Topenot I., Bienvenu J. Monitoring of basophil activation using CD63 and CCR3 in allergy to muscle relaxant drugs // Clin. Immunol. – 2002. – Vol. 102, N 2. – P. 192-199.
33. Murphy E., Shibuya K., Hosken N., Openshaw P., Maino V., Davis K., Murphy K., O'Garra A. Reversibility of T helper 1 and 2 populations is lost after long-term stimulation // J. Exp. Med. – 1996. – Vol. 183, N 3. – P. 901-913.
34. Nagata K., Tanaka K., Ogawa K., Kemmotsu K., Imai T., Yoshie O., Abe H., Tada K., Nakamura M., Sugamura K., Takano S. Selective expression of a novel surface molecule by human Th2 cells in vivo // J. Immunol. – 1999. – Vol. 162. – P. 1278-1286.
35. Ooi K.G., Galatowicz G., Towler H.M., Lightman S.L., Calder V.L. Multiplex cytokine detection versus ELISA for aqueous humor: IL-5, IL-10, and IFNgamma profiles in uveitis // Invest. Ophthalmol. Vis. Sci. – 2006. – Vol. 47, N 1. – P. 272-277.
36. Perniola R., Lobreglio G., Rosatelli M.C., Pitotti E., Accogli E., De Rinaldis C. Immunophenotypic characterisation of peripheral blood lymphocytes in autoimmune polyglandular syndrome type 1: clinical study and review of the literature // J. Pediatr. Endocrinol. Metab. – 2005. – Vol. 18, N 2. – P. 155-164.
37. Picker L.J., Singh M.K., Zdraveski Z., Treer J.R., Waldrop S.L., Bergstresser P.R., Maino V.C. Direct demonstration of cytokine synthesis heterogeneity among human memory/effector T cells by flow cytometry // Blood. – 1995. – Vol. 86, N 4. – P. 1408-1419.
38. Quint D.J., Bolton E.J., McNamee L.A., Solari R., Hissey P.H., Champion B.R., MacKenzie A.R., Zanders E.D. Functional and phenotypic analysis of human T-cell clones which stimulate IgE production in vitro // Immunology. – 1989. – Vol. 67, N 1. – P. 68-74.
39. Read S., Powrie F. CD4 (+) regulatory T cells // Curr. Opin. Immunol. – 2001. – Vol. 13. – P. 644-649.
40. Ryan D.H., Nuccie B.L., Ritterman I., Liesveld J.L., Abboud C.N., Insel R.A. Expression of interleukin-7 receptor by lineage-negative human bone marrow progenitors with enhanced lymphoid proliferative potential and B-lineage differentiation capacity // Blood. – 1997. – Vol. 89, N 3. – P. 929-940.
41. Schubert L.A., Jeffery E., Zhang Y., Ramsdell F., Ziegler S.F. Scurfin (FOXP3) acts as a repressor of transcription and regulates T cell activation // J. Biol. Chem. – 2001. – Vol. 276, N 40. – P. 37672-37679.
42. Seddiki N., Santner-Nanan B., Martinson J., Zaunders J., Sasson S., Landay A., Solomon M., Selby W., Alexander S.I., Nanan R., Kelleher A., Fazekas de St Groth B. Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells // J. Exp. Med. – 2006. – Vol. 203, N 7. – P. 1693-1700.
43. Shevach E.M. CD4+CD25+ suppressor T cells: more questions than answers // Nature Reviews Immunology. – 2002. – Vol. 2 – P. 389-400.
44. Takahashi T., Sakaguchi S. The role of regulatory T cells in controlling immunologic self-tolerance // Int. Rev. Cytol. – 2003. – Vol. 225 – P. 1-32.
45. Waldmann H., Graca L., Cobbold S., Adams E., Tone M., Tone Y. Regulatory T cells and organ transplantation // Semin. Immunol. – 2004. – Vol. 16, N 2. – P. 119-126.
46. Wherry E.J., Ahmed R. Memory CD8 T-cell differentiation during viral infection // J. Virol. – 2004. – Vol. 78. – P. 5535-5545.
47. Xu L., Zha Q., Sun1 H., Jia Q., Li F., He X. Preparation and Characterization of HLA-A*0201 Tetramer Loaded with IE-1316-324 Antigenic Peptide of Human Cytomegalovirus // Cell. Mol. Immunol. – 2006. – Vol. 3, N 5. – P. 367-371.
48. Yagi H., Nomura T., Nakamura K., Yamazaki S., Kitawaki T., Hori S., Maeda M., Onodera M., Uchiyama T., Fujii S., Sakaguchi S. Crucial role of FOXP3 in the development and function of human CD25+CD4+ regulatory T cells // Int. Immunol. – 2004. – Vol. 16, N 11. – P. 1643-1656.
49. Yuan J., Latouche J.B., Hodges J., Houghton A.N., Heller G., Sadelain M., Riviere I., Young J.W. Langerhans-type dendritic cells genetically modified to express full-length antigen optimally stimulate CTLs in a CD4-dependent manner // J. Immunol. – 2006. – Vol. 176, N 4. – P. 2357-2365.
50. Zaunders J.J., Dyer W.B., Munier M.L., Ip S., Liu J., Amyes E., Rawlinson W., De Rose R., Kent S.J., Sullivan J.S., Cooper D.A., Kelleher A.D. CD127+CCR5+CD38+++ CD4+ Th1 effector cells are an early component of the primary immune response to vaccinia virus and precede development of interleukin-2+ memory CD4+ T cells // J. Virol. – 2006. – Vol. 80, N 20. – P. 10151-10161.
51. Zola H., Swart B., Nicholson I., Aasted B., Bensussan A., Boumsell L., Buckley C., Clark G., Drbal K., Engel P., Hart D., Horejsi V., Isacke C., Macardle P., Malavasi F., Mason D., Olive D., Saalmueller A., Schlossman S.F., Schwartz-Albiez R., Simmons P., Tedder T.F., Uguccioni M., Warren H. CD molecules 2005: human cell differentiation molecules // Blood. – 2005. – Vol. 106, N 9. – P. 3123-3126.
Review
For citations:
Khaidukov S.V., Zurochka A.V. EXPANSION OF OPPORTUNITIES OF THE FLOW CYTOMETRY METHOD FOR CLINICAL AND IMMUNOLOGYCAL PRACTICE. Medical Immunology (Russia). 2008;10(1):5-12. (In Russ.) https://doi.org/10.15789/1563-0625-2008-1-5-12
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