A STUDY OF IMMUNOGENIC AND PROTECTIVE PROPERTIES OF THE HEAT-STABLE LETHAL TOXIN OF YERSINIA PSEUDOTUBERCULOSIS AND ITS EFFECTS UPON HEMATOLOGICAL AND BLOOD CYTOKINE PARAMETERS OF LABORATORY MICE
https://doi.org/10.15789/1563-0625-2014-3-227-236
Abstract
The article presents some data concerning antigenic and immunogenic properties of the lethal heat-stable toxin (HST) from Yersinia pseudotuberculosis, a protein with molecular weight of 45 kDa. The mice,
following double immunization with HST at a dose of 0.1 mg per mouse, displayed higher antibody production, in comparison with a dose of 0.01 mg/mouse. The appropriate differences were revealed with regard of
leukocyte responses, i.e., development of leukopenia, neutropenia, lymphopenia upon immunization with the 0.01 mg of HST per mouse, whereas leukocytosis, and increase in lymphocytes and monocytes was detected after a dose of 0.1 mg/mouse. We detected some doseependent differences in cytokine-modulating activity. I.e., at HST dose of 0.01 mg per mouse, we detected mostly proinflammatory, acutehase responses, whereas a dose of 0.1 mg/mice caused induction of . IFNγ and cytokines promoting lymphocyte proliferation and antibody production by day +17. Upon double immunization of mice, the toxin showed protective properties when injecting them with lethal dose of Y. pseudotuberculosis. A lagging activation of antibody producers during
HST response suggests a need for searching effective adjuvant tools of enhancement and acceleration of specific humoral immune reactions against this antigen.
About the Authors
A. V. Tsybulsky
Far Eastern Federal University, Vladivostok, Russian Federation
Russian Federation
Russian Federation
PhD (Medicine), Associate Professor, Department of Biochemistry, Microbiology and Biotechnology, Far Eastern Federal University
N. F. Timchenko
Far Eastern Federal University, Vladivostok, Russian Federation
Russian Federation
Russian Federation
PhD, MD (Medicine), Professor,Head, Laboratory of Molecular Basis of Pathogenicity of Bacteria, G.P. Somov Research Institute of Epidemiology and Microbiology, Siberian Branch of Russian Academy of Medical Sciences
E. Ya. Kostetsky
G.P. Somov Research Institute of Epidemiology and Microbiology, Siberian Branch of Russian Academy of Medical Sciences, Vladivostok
Russian Federation
Russian Federation
PhD, MD (Biology), Professor, Head, Department of Biochemistry, Microbiology and Biotechnology, Far Eastern Federal University
N. S. Vorobieva
Far Eastern Federal University, Vladivostok, Russian Federation
Russian Federation
Russian Federation
Postgraduate Student, Department of Biochemistry, Microbiology and Biotechnology, Far Eastern Federal University
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Review
For citations:
Tsybulsky A.V., Timchenko N.F., Kostetsky E.Ya., Vorobieva N.S. A STUDY OF IMMUNOGENIC AND PROTECTIVE PROPERTIES OF THE HEAT-STABLE LETHAL TOXIN OF YERSINIA PSEUDOTUBERCULOSIS AND ITS EFFECTS UPON HEMATOLOGICAL AND BLOOD CYTOKINE PARAMETERS OF LABORATORY MICE. Medical Immunology (Russia). 2014;16(3):227-236. (In Russ.) https://doi.org/10.15789/1563-0625-2014-3-227-236
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