ACTIVATION AND DIFFERENTIATION OF PERIPHERAL BLOOD MONOCYTES IN PERIODONTITIS
https://doi.org/10.15789/1563-0625-2014-2-165-172
Abstract
Periodontitis is a chronic infectious inflammatory disease, being the most common bone disease in humans. Porphyromonas gingivalis bacteria are quite common in periodontal lesions, thus considered among major causal agents of periodontitis. Monocytes play a crucial role in development of the infectious events and subsequent inflammation. The aim of this study was to investigate appropriate changes of the monocyte subpopulations, as well as to assess relationships between expression of CD14, CD16, CD45RA, HLA-DR on the monocyte surfaces in the patients with periodontitis. It was demonstrated that periodontitis is accompanied by increased counts of pro-inflammatory monocytes. These changes are characterized by decreased expression of CD14, along with occurence of CD16 marker. Concomitant activation of the monocytes is reflected by CD45RA expression. However, in spite of significant increase in CD14lowCD16+HLA-DR+ cell counts observed in patients with periodontitis, these monocyte populations are not likely to play a significant role in development of a specific immune response and suppression of periodontitis.
About the Authors
L. M. Tebloeva
Moscow A.I. Evdokimov University of Medical Dentistry, Moscow
Russian Federation
Russian Federation
PhD (Medicine), Doctoral Candidate, Faculty of Postgraduate Education, Moscow A.I. Evdokimov University of Medical Dentistry
O. A. Guseva
Dmitry Rogachev Federal Research Clinical Center of Pediatric Hematology, Oncology and Immunology, Ministry of Health Care, Moscow
Russian Federation
Russian Federation
PhD (Biology), Senior Research Associate, Laboratory of Cellular Immunology, Dmitry Rogachev Federal Research Clinical Center of Pediatric Hematology, Oncology and Immunology, Ministry of Health Care
S. V. Philippov
Russian Children’s Clinical Hospital, Ministry of Health Care, Moscow
Russian Federation
Russian Federation
Medical Doctor (Clinical Laboratory Diagnostics), Russian Children’s Clinical Hospital, Russian Ministry of Health Care
S. V. Khaidukov
M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bio-Organic Chemistry, Russian Academy of Sciences, Moscow
Russian Federation
Russian Federation
PhD, MD (Biology), Head of Laboratory, Senior Research Associate, M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bio-Organic Chemistry, Russian Academy of Sciences
References
1. Khaidukov S.V., Baydun L.A., Zurochka A.V., Totolian Areg A. Standartizovannaya tekhnologiya «Issledovanie subpopulyatsionnogo sostava limfotsitov perifericheskoy krovi s primeneniem protochnykh tsitoflyuorimetrov-analizatorov» (Proekt) [A standardized technology «research subpopulation of lymphocytes in peripheral blood using flow cytometry analyzers» (The project)]. Meditsinskaya immunologiya – Medical Immunology, 2012, vol. 14(3), pp. 255-268.
2. Chukhlovin A.B., Solov`eva A.M., Matelo S.K., Kobiyasova I.V., Morozova E.B., Hokhlacheva A.V., Teplyakov B.G., Sysoev K.A., Konstantinova V.E., Matelo L.N., Totolian A.A. Mikrobnye markery zabolevaniy parodonta i ikh prakticheskaya znachimost` v stomatologii [Bacterial markers of periodontal diseases and their practical significance in dent istry]. Byulleten` eksperimental`noy biologii i meditsiny – Bulletin of Experimental Biology and Medicine. – 2007. – Vol. 144(10), – P. 427-431
3. Almeida J., Bueno C., Algueró M.C., Sanchez M.L., de Santiago M. Comparative analysis of the morphological, cytochemical, immunophenotypical, and functional characteristics of normal human peripheral blood lineage (-)/CD16(+)/HLA-DR(+)/CD14(-/lo) cells, CD14(+) monocytes, and CD16(-) dendritic cells. Clin. Immunol., 2001, vol. 100, no. 3, pp. 325-338.
4. Brohee D., Higuet N. In vitro stimulation of peripheral blood mononuclear cells by phytohaemagglutinin A induces CD45RA expression on monocytes. Cytobios., 1992, vol. 71, pp. 105-111.
5. Buduneli N., Bicakci N., Keskinoglu A. Flow-cytometric analysis of lymphocyte subsets and mCD14 expression in patients with various periodontitis categories. J. Clin. Periodontol., 2001, vol. 28, pp. 419-424.
6. Fearon D.T., Locksley R.M. The instructive role of innate immunity in the acquired immune responses. Science, 1996, vol. 272, pp. 50-54.
7. Fingerle G., Pforte A., Passlick B., Blumenstein M., Strobel M., Ziegler-Heitbrock H.W. The novel subset of CD14+/CD16+blood monocytesis expanded in sepsis patients. Blood, 1993, vol. 82, pp. 3170-3176.
8. Gabriel H., Urhausen A., Brechtel L., M ller H.J., Kindermann W. Alterations of regular and mature monocytes are distinct, and dependent of intensity and duration of exercise. Eur. J. Appl. Physiol. Occup. Physiol., 1994, vol. 69, no. 2, pp. 179-181.
9. Kanaya S., Nemoto E., Ogawa T., Shimauchi H. Porphyromonas gingivalis lipopolysaccharides induce maturation of dendritic cells with CD14+CD16+ phenotype. Eur. J. Immunol., 2004, vol. 34, pp. 1451-1460.
10. Rothe G., Gabriel H., Kovacs E., Klucken J., St hr J., Kindermann W., Schmitz G. Peripheral blood mononuclear phagocyte subpopulations as cellular markers in hypercholesterolemia. Arterioscler. Thromb. Vasc. Biol., 1996, vol. 16, pp. 1437-1447.
11. Taubman M.A., Kawai T. Involvement of T-lymphocytes in periodontal disease and in direct andindirect induction of bone resorption. Crit. Rev. Oral. Biol. Med., 2001, vol. 12, pp. 125-135.
12. Wood G.S., Freudenthal P.S., Edinger A., Steinman R.M., Warnke R.A. CD45 epitope mapping of human CD1a+ dendritic cells and peripheral blood dendritic cells. Am. J. Pathol., 1991, vol. 138(6), pp. 1451- 1459.
13. Ziegler-Heitbrock H.W.L., Strobel M., Kieper D., Fingerle G., Schlunck T., Petersmann I., Ellwart J., Blumenstein M., Haas J.G. Differential expression of cytokines in human blood monocytes. Blood, 1992, vol. 79, pp. 503-511.
14. Ziegler-Heitbrock H.W.L., Ulevitch R.J. CD14: Cell surface receptor and differentiation marker. Immunol. Today, 1993, vol. 14, pp. 121-125.
15. Ziegler-Heitbrock H.W.L. Heterogeneity of human blood monocytes: the CD14+CD16+ subpopulation. Immunol. Today, 1996, vol. 17, pp. 424-428.
16. Ziegler-Heitbrock H.W.L. The CD14+CD16+ blood monocytes: their role in infection and inflammation. J. Leukoc. Biol., 2007, vol. 81, pp. 584-592.
Review
For citations:
Tebloeva L.M., Guseva O.A., Philippov S.V., Khaidukov S.V. ACTIVATION AND DIFFERENTIATION OF PERIPHERAL BLOOD MONOCYTES IN PERIODONTITIS. Medical Immunology (Russia). 2014;16(2):165-172. (In Russ.) https://doi.org/10.15789/1563-0625-2014-2-165-172
Views:
1354
Email this article 