REGULATORY MECHANISMS OF HUMAN MONOCYTIC THP-1 CELLS TRANSENDOTHELIAL MIGRATION INDUCED BY STREPTOCOCCUS PYOGENES COMPONENTS
https://doi.org/10.15789/1563-0625-2013-5-457-464
Abstract
Abstract. Enhanced transendothelial migration of leukocytes during infection and inflammation occurs due to increased expression of ICAM-1 and VCAM-1 adherence molecules of the immunoglobulin superfamily, and increased secretion of chemokines by endothelial cells. Our previous in vitro studies with human monocytic THP-1 cells showed that some components of Streptococcus pyogenes caused a sufficient enhancement of transendothelial cell migration. However, expression levels of adhesion molecules remained unchanged on endothelial cells. The purpose of the work was to study mechanisms underlying the enhanced transendothelial migration of THP-1 cells treated with supernatante of streptococcal cells (SSC). SSC exhibited chemoattractive activity towards THP-1 cells. SSC increased chemotaxis and transmigration of THP-1 cells. Meanwhile, a 24-hour preincubation of THP-1 cells with SSC caused suppression of the both effects, being accompanied by decreased level of Focal Adhesion Kinase phosphorylation. Preincubation of THP-1 cells with pertussis toxin did reduce the transmigration intensity in presence of the SSC down to the level of spontaneous migration. These results suggest that increased transmigration of THP-1 cells under the influence of SSC may be determined by its chemoattractive action upon the migrating cells.
About the Authors
E. A. Starikova
Research Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg
Russian Federation
Russian Federation
PhD, Senior Research Associate, Department of Immunology
197376, Acad. Pavlov str., 12. Phone: 7 (812) 234-16-69
A. M. Lebedeva
Research Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg
Russian Federation
Russian Federation
Postgraduate Student, Research Associate, Department of Immunology
N. A. Aktureeva
St. Petersburg State Institute of Technology (Technical University), St. Petersburg
Russian Federation
Student
Russian Federation
Student
L. A. Burova
Research Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg
Russian Federation
Russian Federation
PhD, MD (Medicine), Leading Research Associate, Department of Microbiology
I. S. Freidlin
Research Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg
Russian Federation
Russian Federation
PhD, MD (Medicine), Corresponding Member, Russian Academy of Medical Sciences, Chief, Department of Immunology
References
1. Лебедева А.М., Старикова Э.А., Бурова Л.А., Фрейдлин И.С. Компоненты Streptococcus pyogenes изменяют активность адгезии моноцитов к эндотелиальным клеткам // Медицинская иммунология. – 2011. –Т. 13, № 4-5. – С. 321-322. Lebedeva A.M., Starikova E.A., Burova L.A., Freydlin I.S. Komponenty Streptococcus pyogenes izmenyayut aktivnost` adgezii monotsitov k endotelial`nym kletkam [Components of Streptococcus pyogenes alter the activity of monocytes adhesion to the endothelial cells]. Meditsinskaya immunologiya – Medical Immunology , 2011, vol. 13, no. 4-5, pp. 321-322.
2. Старикова Э.А., Соколов Д.И., Чернова А.А., Бурова Л.А., Сельков С.А., Фрейдлин И.С. Влияние бактериальных лигандов паттерн распознающих рецепторов моноцитоподобных клеток THP-1 на их трансэндотелиальную миграцию // Медицинская иммунология. – 2008. – Т. 10, № 6. – С. 605-613. Starikova E.A., Sokolov D.I., Chernova A.A., Burova L.A., Sel`kov S.A., Freydlin I.S. Vliyanie bakterial`nykh ligandov pattern raspoznayushchikh retseptorov monotsitopodobnykh kletok THP-1 na ikh transendotelial`nuyu migratsiyu [Influence of bacterial ligands pattern recognition receptors on the THP-1 cells transendothelial migration]. Meditsinskaya immunologiya – Medical Immunology, 2008, vol. 10, no. 6, pp. 605-613.
3. Старикова Э.А., Фрейдлин И. С., Соколов Д.И., Сельков С.А. Изменения свойств эндотелиальных клеток линии EA.hy.926 под влиянием TNFальфа, IFNгамма и IL-4 // Иммунология. – 2005. – № 2. – C. 83-87. Starikova E.A., Freydlin I.S., Sokolov D.I., Sel`kov S.A. Izmeneniya svoystv endotelial`nykh kletok linii EA.hy.926 pod vliyaniem TNFal`fa, IFNgamma i IL-4 [Endothelial cells line EA.hy.926 properties alterations under the influence of TNFα, IFNγ and IL-4]. Immunologiya – Immunology, 2005, no. 2, pp. 83-87.
4. Blander J.M., Sander L.E. Beyond pattern recognition: five immune checkpoints for scaling the microbial threat. Nature reviews, 2012, vol. 12, pp. 215-225.
5. Bhavsar A.P., Guttman J.A., Finlay B.B. Manipulation of host-cell pathways by bacterial pathogens. Nature, 2007, vol .449, pp. 827-834.
6. Bokoch M.G. Chemoattractant Signaling and Leukocyte Activation. Blood, 1995, vol. 86, no. 5, pp. 1649-1660.
7. Imhof B.A., Aurrand-Lions M. Adhesion mechanisms regulating the migration of monocytes. Nature reviews, 2004, vol. 4, pp. 432-444.
8. Johnston B., Butcher E.C. Chemokines in rapid leukocyte adhesion triggering and migration. Seminars in immunology, 2002, vol. 14, pp. 83-92.
9. Le Y., Li B., Gong W., Shen W., Hu J., Dunlop N.M., Oppenheim J.J., Wang J.M. Novel pathophysiological role of classical chemotactic peptide receptors and their communications with chemokine receptors. Immunological Reviews, 2000, vol. 177, pp. 185-194.
10. Ley K., Laudanna C., Cybulsky M.I., Nourshargh S. Getting to the site of inflammation: the leukocyte adhesion cascade updated. Nat. Rev. Immunol., 2007, vol. 7, no. 9, pp. 678-689.
11. Mantovani A., Bussolino F., Martino I. Cytokine regulation endothelial cell function: from molecular level to bedside. Immunology today, 1997, vol. 1, pp. 231-239.
12. Fernandez-Borja M., van Buul J.D., Hordijk P.L. The regulation of leucocyte transendothelial migration by endothelial signalling events. Cardiovascular Research, 2010, vol. 86, pp. 202-210.
13. Middleton J., Patterson A.M., Gardner L., Schmutz C., Ashton B.A. Leukocyte extravasation: chemokine transport and presentation by the endothelium. Blood, 2002, vol. 100, no. 12, pp. 3853-3860.
14. Petri B., Phillipson M., Kubes P. The physiology of leukocyte recruitment: an in vivo perspective. The Journal of Immunology, 2008, vol. 180, pp. 6439-6446.
15. Tomar A., Schlaepfer D.D. Focal adhesion kinase: switching between GAPs and GEFs in the regulation of cell motility. Curr. Opin. Cell Biol., 2009, vol. 21, no. 5, pp. 676-683.
Review
For citations:
Starikova E.A., Lebedeva A.M., Aktureeva N.A., Burova L.A., Freidlin I.S. REGULATORY MECHANISMS OF HUMAN MONOCYTIC THP-1 CELLS TRANSENDOTHELIAL MIGRATION INDUCED BY STREPTOCOCCUS PYOGENES COMPONENTS. Medical Immunology (Russia). 2013;15(5):457-464. (In Russ.) https://doi.org/10.15789/1563-0625-2013-5-457-464
Views:
1051
Email this article 