EFFECTS OF MYOCARDIAL CYTOSOLIC FRACTION AND LIPOPOLYSACCHARIDE UPON MONOCYTIC FUNCTIONS
https://doi.org/10.15789/1563-0625-2013-5-439-448
Abstract
Abstract. Complicated systemic inflammatory response syndrome in patients undergone open-heart surgery is an important issue of cardiac surgery. The conditions and trigger mechanisms leading to such a complication remain unclear.
We studied the impact of mechanincal myocardial injury products released into blood during open-heart surgery, lipopolysaccharides and their combination on isolated monocytes.
It was found that mechanically injured myocardial tissue can be a source of intracellular heat shock protein 70 (Hsp70). The content of Hsp70 in the cytosolic cardiomyocyte fraction responsible for mechanical myocardial injury modeling corresponds to the level of proinflammatory cytokine production by monocytes and the density of TLR4 surface expression. The study results confirm the synergy and potentiation of the combined impact of mechanical myocardial injury products and lipopolysaccharides on the levels of cytokine production by monocytes.
About the Authors
V. G. Matveeva
Research Institute for Complex Problems of Cardiovascular Diseases, Russian Academy of Medical Sciences, Siberian Branch, Kemerovo
Russian Federation
Russian Federation
Research Associate, Cell Technology Laboratory, Department of Experimental and Clinical Cardiology
665002, Sosnovy blvrd, 6. Phone: 7 (3842) 64-41-56
A. S. Golovkin
Research Institute for Complex Problems of Cardiovascular Diseases, Russian Academy of Medical Sciences, Siberian Branch, Kemerovo
Russian Federation
Russian Federation
PhD (Medicine), Chief, Department of Experimental and Clinical Cardiology
M. N. Chernova
Research Institute for Complex Problems of Cardiovascular Diseases, Russian Academy of Medical Sciences, Siberian Branch, Kemerovo
Russian Federation
Russian Federation
Researcher, Cell Technology Laboratory, Department of Experimental and Clinical Cardiology
I. V. Kudryavtsev
Research Institute of Experimental Medicine, Russian Academy of Medical Sciences, North Western Branch, St. Petersburg
Russian Federation
PhD (Biology), Senior Research Associate
Russian Federation
PhD (Biology), Senior Research Associate
S. V. Ivanov
Research Institute for Complex Problems of Cardiovascular Diseases, Russian Academy of Medical Sciences, Siberian Branch, Kemerovo
Russian Federation
Russian Federation
PhD, MD (Medicine), Chief, Reconstructive surgery laboratory
E. V. Grigoriev
Research Institute for Complex Problems of Cardiovascular Diseases, Russian Academy of Medical Sciences, Siberian Branch, Kemerovo
Russian Federation
PhD, MD (Medicine), Professor, Deputy Director
Russian Federation
PhD, MD (Medicine), Professor, Deputy Director
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Review
For citations:
Matveeva V.G., Golovkin A.S., Chernova M.N., Kudryavtsev I.V., Ivanov S.V., Grigoriev E.V. EFFECTS OF MYOCARDIAL CYTOSOLIC FRACTION AND LIPOPOLYSACCHARIDE UPON MONOCYTIC FUNCTIONS. Medical Immunology (Russia). 2013;15(5):439-448. (In Russ.) https://doi.org/10.15789/1563-0625-2013-5-439-448
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