INFLUENCE OF SOLUBLE PLACENTAL TISSUE-DERIVED MOLECULES UPON EXPRESSION OF ADHESION MOLECULES BY EA.HY926 ENDOTHELIAL CELLS

Abstract.  Leukocyte  recruitment  to  placental  tissue  is  an  important  factor  of  its  development.  In  this respect, adhesion molecules at the endothelial cell surface represent a key determining factor of leukocyte adhesion and their trans-endothelial migration. The goal of investigation was to evaluate changed expression of adhesion molecules on the endothelial cells induced by supernates of placental tissue cultures. Placental tissue supernatants produced by the first- and third-trimester placental tissue from normal pregnancy, as well as from women with gestosis, induced higher expression of CD31, CD9, CD62E, CD62P, CD34, CD54, CD51/61, CD49d  and  integrin  β7  expression  by  endothelial  cells,  as  compared  with  their  baseline  levels.  However, the  supernates  from  pre-eclamptic  placental  tissue (3rd  trimester)  caused  an  increased  CD9  expression by  endothelial  cells,  as  compared  with  effects  of placental  supernates  from  eclampsia-free  cases.  Our data  contribute  to  understanding  a  possible  role  of endothelial cell adhesion molecules in recruitment of leukocytes to placental tissue and possible participation of adhesion molecules in pathogenesis of pre-eclampsia. The work was supported by a grant from Russian Ministry of Education and Science ГК №02.740.11.0711 and Presidential grant № НШ-3594.2010.7 and МД-150.2011.7. (Med. Immunol., 2011, vol. 13, N 6, pp 589-596)

1. Соколов Д.И., Колобов А.В., Лесничия М.В., Костючек И.Н., Степанова О.И., Кветной И.М., Сельков С.А. Механизмы регуляции апоптоза в ткани плаценты при физиологической беременности и при беременности, осложненной гестозом // Бюллетень экспериментальной биологии и медицины. – 2009. – Т. 148, № 11. – С. 519-523.

2. Соколов Д.И., Лесничия М.В., Селютин А.В., Климова В.А., Аржанова О.Н., Сельков С.А. Роль цитокинов в контроле развития плаценты в норме и при гестозе // Иммуноло-гия. – 2009. – № 1. – С. 22-26.

3. Старикова Э.А., Амчиславский Е.И., Соколов Д.И., Фрейдлин И.С., Полосухина Е.Р., Барышников А.Ю. Изменение поверхностного фенотипа эндотелиальных клеток под влиянием провоспалительных и противовоспалительных цитокинов // Медицинская иммунология. – 2003. – Т. 5, № 1-2. – С. 39-48.

4. Фрейдлин И.C., Тотолян А.А. Клетки иммунной системы. – СПб.: Наука, 2000. – 231 с.

5. Ширшев С.В. Иммунология материнско-фетальных взаимодействий. Екатеринбург, 2009. – 582 с.

6. Barber D. F., Long E. O. Coexpression of CD58 or CD48 with intercellular adhesion molecule 1 on target cells enhances adhesion of resting NK cells // J. Immunol. – 2003. – Vol. 170. – P. 294-299.

7. Bardin N., Anfosso F., Masse J.-M., Cramer E., Sabatier F., Le Bivic A., Sampol J.,Dignat-George F. Identification of CD146 as a component of the endothelial junction involved in the control of cell-cell cohesion // Blood. – 2001. – Vol. 98. – P. 3677-3684.

8. Benyo D.F., Smarason A., Redman C.W.G., Sims C., Conrad K.P. Expression of inflammatory cytokines in placentas from women with preeclampsia // J. Clin. Endocrinol. Metab. – 2001. – Vol. 86. – P. 2505-2512.

9. Bowen J.A., Hunt J.S. The role of integrins in reproduction // P.S.E.B.M. – 2000. – Vol. 223. – P. 331-343.

10. Cao G., O’Brien C.D., Zhou Z., Sanders S.M., Greenbaum J.N., Makrigiannakis A., Delisser H.M. Involvement of human PECAM-1 in angiogenesis and in vitro endothelial cell migration // Am J Physiol Cell Physiol. – 2002. – Vol. 282. – P. 1181-1190.

11. Cartwright J.E., Fraser R., Leslie K., Wallace A.E., James J.L. Remodelling at the maternal–fetal interface: relevance to humanpregnancy disorders // Reproduction. – 2010. – Vol. 140. – P. 803-813.

12. De Oliveira L.G., Lash G.E., MurrayDunning C., Bulmer J.N., Innes B.A., Searle R.F.,Sass N., Robson S.C. Role of interleukin 8 in uterine natural killer cell regulation of extravillous trophoblast cell invasion// Placenta. – 2010. –Vol. 31. – P. 595-601.

13. Dimitriadis E., White C.A., Jones R.L., Salamonsen L.A. Cytokines, chemokines and growth factors in endometrium related to implantation //Human Reproduction Update. – 2005. – Vol. 11, N 6. – P. 613-630.

14. Drake P. M., Gunn M.D., Charo I.F., Tsou C.-L., Zhou Y., Huang L., Fisher S.J. Human placental cytotrophoblasts attract monocytes and CD56 bright Natural Killer cells via the actions of monocyte inflammatory protein 1 a // J. Exp. Med. – 2001. – Vol. 193, N 10. – P. 1199-1212.

15. Fujiwara T., Taketani Y. Demonstration of angiogenin in human endometrium and its enhanced expression in endometrial tissues in the secretory phase and the decidua //The Journal of Clinical Endocrinology & Metabolism. – 2001. – Vol 86, N 11. – P. 5609-5614.

16. Gomez-Lopez N., Guilbert L.J., Olson D.M Invasion of the leukocytes into the fetal-maternal interface during pregnancy // J. Leukoc. Biol. – 2010. – Vol. 88. – P. 1-9.

17. Granger J.P., Alexander B.T., Llinas M.T., Bennett W.A., Khalil R.A. Pathophysiology of hypertension during preeclampsia linking placental ischemia with endothelial dysfunction // Hypertension. – 2001. – Vol. 38. – P. 718-722

18. Huang Y., Zhu X.-Y., Du M.-R., Li D.-J. Human trophoblasts recruited T lymphocytes and Monocytes into decidua by secretion of chemokine CXCL16 and interaction with CXCR6 in the firsttrimester pregnancy // The Journal of Immunology – 2008. – Vol. 180. – P. 2367-2375.

19. Hung T.-H., Charnock-Jones D.S., Skepper J.N., Burton G.J. Secretion of tumor necrosis factor-α from human placental tissues induced by hypoxia-reoxygenation causes endothelial cell activation in vitro: A potential mediator of the inflammatory response in preeclampsia // American Journal of Pathology. – 2004. – Vol. 164, N 3. – P. 1049-1061.

20. Irminger-Fingera I., Jastrow N., Irion O. Preeclampsia: A danger growing in disguise // The International Journal of Biochemistry & Cell Biology. – 2008. – Vol. 40. – P. 1979-1983

21. Kai K., Nasu K., Nakamura S., Fukuda J., Nishida M., Miyakawa I. expression of interferonγ-indicible protein-10 in human endometrial stromal cells // Molecular Human Reproduction. – 2002. – Vol. 8, N 2. – P. 176-180.

22. Koga K., Osuga Y., Tsutsumi O., Yano T., Yoshino O., Takai Y., Matsumi H., Hiroi H., Kugu K., Momoeda M. Demonstration of angiogenin in human endometrium and its enhanced expression in endometrial tissues in the secretory phase and the deciduas // J. Clin Endocrinol Metab. – 2001. – Vol. 86. – P. 5609-5614. 23. Kruse A., Martens N., Fernekorn U., Hallmann R., Butcher E.C. Alterations in the expression of homing-associated molecules at the maternal/fetal interface during the course of pregnancy // Biology of reproduction. – 2002. – Vol. 66. – P. 333-345

23. Laresgoiti-Servitje E., Goґmez-Loґpez N., Olson D.M. An immunological insight into the origins of pre-eclampsia //Human Reproduction Update. – 2010. – Vol. 16, N 5. – P. 510–524.

24. Lash G.E., Naruse K., Innes B.A., Robson S.C., Searle R.F., Bulmer J.N. Secretion of angiogenic growth factors by villous cytotrophoblast and extravillous trophoblast in early human pregnancy // Placenta. – 2010. – Vol. 31. – P. 545-548.

25. Lyall F., Simpson H., Bulmer J.N., Barber A., Robson S.C. Transforming growth factor-β expression in human placenta and placental bed in third trimester normal pregnancy, preeclampsia, and fetal growth restriction // Am J. Pathol. – 2001. – Vol. 159, N 5. – P. 1827-1838.

26. Mellembakken J.R., Aukrust P., Olafsen M.K., Ueland T., Hestdal K., Videm V. Activation of leukocytes during the uteroplacental passage in preeclampsia // Hypertension. – 2002. – Vol. 39. – P. 155-160.

27. Park S.Y., DiMaio T. A., Scheef E. A., Sorenson C.M., Sheibani N. PECAM-1 regulates proangiogenic properties of endothelial cells through modulation of cell-cell and cell-matrix interactions // Am J. Physiol Cell Physiol. – 2010. –Vol. 299, N 6. – P. 1468-C1484.

28. Sato Y., Higuchi T., Yoshioka S., Tatsumi K., Fujiwara H., Fujii S. Trophoblasts acquire a chemokine receptor, CCR1, as they differentiate towards invasive phenotype // Development. – 2003. – Vol. 130 – P. 5519-5532.

29. Scaife P.J., Bulmer J.N., Robson S.C., Innes B.A., Searle R.F. Effector activity of decidual CD8+ T lymphocytes in early human pregnancy // BIOLOGY OF REPRODUCTION. – 2006. – Vol. 75 – P. 562-567.

30. Silva R., D’Amico G., Hodivala-Dilke K.M., Reynolds L.E. Integrins:the keys to unlocking angiogenesis // Arterioscler Thromb Vasc Biol. – 2008. – Vol. 28. –P. 1703-1713.

31. Tomescu C., Law W. K., Kedes D.H. Surface downregulation of major histocompatibility complex Class I, PECAM, and ICAM-1 following de novo infection of endothelial cells with Kaposi’s sarcoma-associated herpesvirus // Journal of virology – 2003. – Vol. 77, N 17. – P. 9669-9684.

32. Wood H. B., May G., Healy L., Enver T., and Morriss-Kay G.M. CD34 expression patterns during early mouse development are related to modes of blood vessel formation and reveal additional sites of hematopoiesis // Blood. – 1997. - Vol. 90, N 6. – P. 2300-2311.

33. Wu X., Li-P. Jin, Min-Min Yuan, Zhu Y., Ming-Yan Wang, and Da-Jin Li1. Human first-trimester trophoblast cells recruit CD56brightCD16 NK cells into decidua by way of expressing and secreting of CXCL12/stromal cell-derived factor 1 // The Journal of Immunology. – 2005. – Vol. 175 – P. 61-68.

34. Zhang F., Kotha J., Jennings L.K., Zhang X.A. Tetraspanins and vascular functions // Cardiovascular Research – 2009. – Vol. 83 – P. 7-15.