SUBPOPULATIONAL FEATURES OF PERIPHERAL BLOOD CELLS IN THE PATIENTS WITH AUTOIMMUNE MYOCARDITIS: CLINICAL AND PATHOGENETIC ASPECTS

Abstract. The goal of our research was comparative study of the most important parameters of subset cytoarchitectonics in the patients with the different courses of myocarditis and evaluation of their pathogenetic and clinical value in the practice of the physician. We have investigated 99 patients with myocarditis and 40 healthy donors. In patients with malignant course of disease we revealed increased activation index of T/B-cells; increased expression of the activation markers on the both lines of differentiation; disproportion in the immunoregulatory subsets with increased role of dendric cells; decreased intensity of the autoreactive T-cells apoptosis. in the patient with the In patients with nonmalignant course of disease expressed signs of immunopathology were not found. Thus, study of activation markers on the cells of the peripheral blood is more informative and noninvasive method of diagnostics of myocarditis.

lymphocytes subpopulation, myocarditis, autoimmunity, flow cytometry

1. Амелюшкина В.А. Биохимические маркеры повреждения миокарда // Клин. лаб. диагностка. — 1999. — №7. — С. 25–32.

2. Барышников А.Ю. Моноклональные антитела серии ИКО к дифференцировочным антигенам лимфоцитов человека // Гематол. трансфузиол. — 1990. — № 8. — С. 4–7.

3. Палеев Н.Р., Палеев Ф.Н. Цитокины и их роль в патогенезе заболевании сердца. Клиническая медицина. — 2004. — Т. 82, № 5. — C. 4–7.

4. Палеев Н.Р., Гуревич М.А. Некоронарогенные заболевания миокарда. Состояние проблемы // Клин. мед. — 1998. — № 9. — С. 4–8.

5. Палеев Ф.Н. Популяционный и субпопуляционный состав и экспрессия активационных маркеров лимфоцитов при инфекционно–аллергическом миокардите // Кардиол. — 1999. — Т. 39, № 8. — С. 53–58.

6. Порядин Г.В., Палеев Н.Р., Казимирский А.Н., Салмаси Ж.М., Макарков А.И., Санина Н.П., Палеев Ф.Н. Динамическая характеристика поверхностного фенотипа лимфоцитов периферической крови у больных мпокардитом // Рос. иммунол. ж. — 1999. — Т.4, № 2. — С. 165–170.

7. Порядин Г.В., Макарков А.И., Салмаси Ж.М. // Пат. физиол. и эксперим. тер. — 1997. — № 1. — С. 42–45.

8. Ройтберг Г.Е., Струтынский. А.В. // “Внутренние болезни. Сердечно–сосудистая система”. М.: Издательство БИНОМ, 2003. — С. 856.

9. Сапрыгин Д.Б., Романов М.Ю. Миокардиальные маркеры. Традиционные и современные тест программы. Диагностическая специфичность // Лабор. мед. — 1999. — №2. — С. 16–23.

10. Ana Paula M.P. Marino, Maria Inкs P. Azevedo, Joseli Lannes–Vieira. Differential Expression of Adhesion Molecules Shaping the T–cell Subset Prevalence during the Early Phase of Autoimmune and Trypanosoma cruzi–elicited Myocarditis // Mem. Inst. Oswaldo Cruz, Rio de Janeiro. — Vol.98, N7. — P. 945–952.

11. Angelini A., Crosato M., Boffa G.M., Calabrese F., Calzolari V., Chioin R., Daliento L., Thiene G. Active versus borderline myocarditis: clinicopathological correlates and prognostic implications // Heart. — 2002. – 87. — P. 210–215.

12. Banchereau J., Pascual V., Palucka A.K. Autoimmunity through cytokine–induced dendritic cell activation // Immunity, 2004. — Vol.20, N5. — P. 539–550.

13. Bohbot A., Feugeas O., Cuillerot J.M., Grosse A., Rusciani I., Faradji A., Oberling F. Peripheral blood CD34+ cells: method of purification and ex vivo expansion // Nouv. Rev. Fr. Hematol., 1995. — Vol.37, N6. — P. 359–365.

14. Boyüm A. Separation of lymphocytes, granulocytes, and monocytes from human blood using iodinated density gradient media // Methods Enzymol., 1984. – Vol.108. — P. 88–102.

15. Brigl M., Brenner M.B. CD1: antigen presentation and T cell function // Annu. Rev. Immunol., 2004. – N22. — P. 817–890.

16. Huber S. T cells in coxsackievirus–induced myocarditis // Viral. Immunol., 2004. — Vol 17, N2. — P. 152–164.

17. Kanda T., Yokoyama T., Ohshima S., Yuasa K., Watanabe T., Suzuki T., Murata K. T–lymphocyte subsets as noninvasive markers of cardiomyopathy // Clin. Cardiol., 1990 Sep. — Vol.13, N9. — P. 617–622.

18. Kaya Z., Afanasyeva M., Wang Y., Dohmen K.M., Schlichting J., Tretter T., Fairweather D., Holers V.M., Rose N.R. Contribution of the innate immune system to autoimmune myocarditis: a role for complement. // Nat Immunol, 2001. — Vol. 2, N8. — P.739–745.

19. Lieberman E.B., Hutchins G.M., Herskowitz A., Rose N.R., Baughman K.L. Clinicopathologic description of myocarditis // J. Am. Coll. Cardiol., 1991 Dec. — Vol. 8, N. 7. — P.1617–1626.

20. Mancini G., Carbonara A.O., Heremans J.F. Immunochemical quantitation of antigens by single radial immunodiffusion // Immunochemistry, 1965 Sep. — Vol. 2, N3. — P.235–254.

21. Mason J.W. Myocarditis // Adv. Intern. Med., 1999. — Vol. 44. — P. 293–310.

22. Metelitsa L.S. Flow cytometry for natural killer T cells: multi–parameter methods for multifunctional cells // Clin. Immunol., 2004. — Vol.110, N3. — P.267–276.

23. Pummerer C.L., Grassl G., Sailer M., Bachmaier K.W., Penninger J.M., Neu N. Cardiac myosin–induced myocarditis: target recognition by autoreactive T cells requires prior activation of cardiac interstitial cells // Lab. Invest., 1996 May. — Vol. 74, N5. — P.845–852.

24. Schmaltz A.A., Kandolf R. Myocarditis in childhood: results of a decade,s research// Klin. padiatr., 2001 Jan–Feb. — Vol. 213, N1. — P. 1–7.

25. Stefan D. Anker, Stephan von Haehling. Inflammatory mediators in chronic heart failure: an overview // Heart, 2004. — Vol. 90. — P. 464–470.

26. Takamoto T., Hori Y., Yokoyama M.M., Koga Y., Toshima H. Lymphocyte subsets in patients with dilated cardiomyopathy and perimyocarditis // J. Clin. Lab. Immunol., 1986. — Vol.19, N3. — P. 113–116.

27. Taylor J.A., Havari E., McInerney M.F., Bronson R., Wucherpfennig K.W., Lipes M.A. A spontaneous model for autoimmune myocarditis using the human MHC molecule HLA–DQ8 // J. Immunol., 2004. — Vol.172, N4. — P. 2651–2658.

28. Toba K., Koike T., Shibata A., Hashimoto S., Takahashi M., Masuko M., Azegami T., Takahashi H., Aizawa Y. Novel technique for the direct flow cytofluorometric analysis of human basophils in unseparated blood and bone marrow, and the characterization of phenotype and peroxidase of human basophils // Cytometry, 1999. — Vol. 35, N 3. — P. 249–259.

29. Willmann K. Flow–cytometric immune function methodology for human peripheral blood dendritic cells // Methods Mol. Biol., 2003. — Vol.215. — P. 41–57.