CARBON MONOXIDE: ITS ROLE IN MITOCHONDRIAL PATHWAY OF APOPTOSIS INDUCTION IN JURKAT CANCER CELLS
https://doi.org/10.15789/1563-0625-2012-4-5-353-358
Abstract
Abstract. This study demonstrates ability of carbon monoxide to trigger mitochondrial pathway of apoptosis induction of Jurcat cells. We have shown that proapoptotic action of carbon monoxide is coupled to permeabilization of cellular mitochondrial membranes. Imbalance in Bcl-2 family of regulatory proteins may be considered among possible reasons of the membrane pore formation. We have shown downregulated cl-2 and Bcl-xl mRNA expression and decreased levels of antiapoptotic proteins, along wih decreased mRNA expression and increase of Bad proapototic protein level in Jurkat cells following incubation with 50 μm of CORM-2, a carbon monoxide donor.
About the Authors
E. G. Starikova
НОЦ молекулярной медицины ГБОУ ВПО «Сибирский государственный медицинский университет»
Министерства здравоохранения и социального развития РФ, г. Томск
Russian Federation
Russian Federation
L. A. Tashireva
НОЦ молекулярной медицины ГБОУ ВПО «Сибирский государственный медицинский университет»
Министерства здравоохранения и социального развития РФ, г. Томск
Russian Federation
Russian Federation
E. V. Beldiagina
НОЦ молекулярной медицины ГБОУ ВПО «Сибирский государственный медицинский университет»
Министерства здравоохранения и социального развития РФ, г. Томск
Russian Federation
Russian Federation
O. A. Vasilieva
НОЦ молекулярной медицины ГБОУ ВПО «Сибирский государственный медицинский университет»
Министерства здравоохранения и социального развития РФ, г. Томск
Russian Federation
Russian Federation
V. V. Novitsky
НОЦ молекулярной медицины ГБОУ ВПО «Сибирский государственный медицинский университет»
Министерства здравоохранения и социального развития РФ, г. Томск
Russian Federation
Russian Federation
N. V. Ryazantseva
НОЦ молекулярной медицины ГБОУ ВПО «Сибирский государственный медицинский университет»
Министерства здравоохранения и социального развития РФ, г. Томск
Russian Federation
Russian Federation
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Review
For citations:
Starikova E.G., Tashireva L.A., Beldiagina E.V., Vasilieva O.A., Novitsky V.V., Ryazantseva N.V. CARBON MONOXIDE: ITS ROLE IN MITOCHONDRIAL PATHWAY OF APOPTOSIS INDUCTION IN JURKAT CANCER CELLS. Medical Immunology (Russia). 2012;14(4-5):353-358. (In Russ.) https://doi.org/10.15789/1563-0625-2012-4-5-353-358
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