EVALUATION OF T-CELL EFFECTOR SUBPOPULATIONS DISTRIBUTION IN CHILDREN AND ADULTS BY MEANS OF INTRACELLULAR STAINING OF CYTOKINES
https://doi.org/10.15789/1563-0625-2012-4-5-295-304
Abstract
Abstract. An intracellular staining technique of marker cytokines was applied for evaluation of quantitative distribution of T-lymphocyte effector subsets in children and adults. The results of this study have shown that the numbers of Th2 lymphocytes in infants (7 to 12 months old) correspond to appropriate normal values in adults, being accompanied by decreased Th1 and Тс1cell contents, whereas absolute amounts of Tc2 lymphocytes proved to be higher than in all other age groups. Absolute counts of Th17 and Tnc17 subsets in infants of 7 to 12 months were higher than in adults. Hence, these infants exhibited a shift of immune response towards Th2 (Tc2) and Th17 (Tnc17), thus presenting a potential factor of Th2-mediated allergic disorders in the children under one year. In children over 1 year, absolute amounts of Th1 and Тс1, as well as Th2 and Тс2 lymphocyte counts did not significantly differ from adult values. Th2 lymphocyte counts among adolescents (15 to 18 years) were decreased, along with relative increase of IL-17А-positive lymphocyte scores, thus reflecting a probable predisposition for autoimmune disorders induced at this age. The age-dependent increase in Th1 and Тс1 counts in children may immediately correlate with formation of T-cell memory pool.
About the Authors
Yu. G. Lagereva
МБУ «Клинико-диагностический Центр», г. Екатеринбург
Russian Federation
Russian Federation
S. V. Menshikov
МБУ «Клинико-диагностический Центр», г. Екатеринбург
Russian Federation
Russian Federation
T. L. Savinova
Управление здравоохранения администрации, г. Екатеринбург
Russian Federation
Russian Federation
J. B. Beykin
МБУ «Клинико-диагностический Центр», г. Екатеринбург
Russian Federation
Russian Federation
V. A. Chereshnev
Институт иммунологии и физиологии УрО РАН, г. Екатеринбург
Russian Federation
Russian Federation
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Review
For citations:
Lagereva Yu.G., Menshikov S.V., Savinova T.L., Beykin J.B., Chereshnev V.A. EVALUATION OF T-CELL EFFECTOR SUBPOPULATIONS DISTRIBUTION IN CHILDREN AND ADULTS BY MEANS OF INTRACELLULAR STAINING OF CYTOKINES. Medical Immunology (Russia). 2012;14(4-5):295-304. (In Russ.) https://doi.org/10.15789/1563-0625-2012-4-5-295-304
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