GATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMA
https://doi.org/10.15789/1563-0625-2010-1-2-21-28
Abstract
The aim of the study is to establish the features of expression of GATA-3 in peripheral lymphocytes from bronchial asthma patients (BA). Material and methods. 10 healthy controls, 15 patients with allergic (atopic) and 15 persons with non-allergic BA were examined. A transcription factor GATA-3 expressed in peripheral lymphocytes was analyzed by Western blot after the lymphocytes were lysed. Preparation of cell lysates, and Western blotting were performed by means of a standard procedure (Amersham). An antibody against GATA-3 (Abcam, UK) was used. Levels of the protein were analyzed versus β-actin levels using anti-actin antibody (Sigma Aldrich, USA). Results. Expression of GATA-3 was significantly increased in lymphocytes of patients with allergic BA as compared to healthy persons and non-allergic BA patients. The level of GATA-3 negatively correlated with the degree of airflow obstruction and positively correlated with dosage of parenteral steroids administered. Conclusion. GATA-3 may play a key role in the pathophysiology of BA. One may suggest that increased expression of GATA-3 transcription factor in atopic BA underlie high levels of Th2-cytokines production in allergic disease
About the Authors
V. N. Mineev
Кафедра госпитальной терапии имени акад. М.В. Черноруцкого Санкт-Петербургского государственного
медицинского университета имени акад. И.П. Павлова
Russian Federation
Russian Federation
L. N. Sorokina
Кафедра госпитальной терапии имени акад. М.В. Черноруцкого Санкт-Петербургского государственного
медицинского университета имени акад. И.П. Павлова
Russian Federation
Russian Federation
M. A. Nyoma
Кафедра госпитальной терапии имени акад. М.В. Черноруцкого Санкт-Петербургского государственного
медицинского университета имени акад. И.П. Павлова
Russian Federation
Russian Federation
V. I. Trofimov
Кафедра госпитальной терапии имени акад. М.В. Черноруцкого Санкт-Петербургского государственного
медицинского университета имени акад. И.П. Павлова
Russian Federation
Russian Federation
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Review
For citations:
Mineev V.N., Sorokina L.N., Nyoma M.A., Trofimov V.I. GATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMA. Medical Immunology (Russia). 2010;12(1-2):21-28. (In Russ.) https://doi.org/10.15789/1563-0625-2010-1-2-21-28
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