A COMPARISON OF DIFFERENT LIPOPOLYSACCHARIDE CHEMOTYPES FROM ESCHERICHIA COLI AND SALMONELLA UPON SYNTHESIS OF TNFα AND IL-6 BY MACROPHAGE-LIKE THP-1 CELLS
https://doi.org/10.15789/1563-0625-2009-6-509-514
Abstract
Abstract. Present study was performed to investigate the influence of polysaccharide fragment or lipid A upon induction of TNFα and IL-6 cytokines. The study was performed with human THP-1 monocytic leukemia cells that were induced to differentiate into macrophage-like cells using PMA treatment. Bacterial lipopolysaccharides from S. typhimurium (S-chemotype form), S. typhimurium SL1181 (R-chemotype, Re-mutant), E. coli O55:B5 (S-chemotype), and E. coli JM103 (R-chemotype, Re-mutant) were used in this study. A decreased molar ratio for lipid A-KDO in S-form of LPS from E. coli is accompanied by diminished TNFα and IL-6 expression. By the contrast, for S-form of LPS from Salmonella, a decrease in lipid A-KDO molar ratio did cause a sufficient enhancement of TNFα expression. A contribution of lipid A structure into biological activity of LPS is more significant for Re-chemotype than for S-chemotype, independently on bacterial species.
About the Authors
E. V. Voloshina
Институт фундаментальных проблем биологии РАН, г. Пущино
Russian Federation
Russian Federation
S. V. Zubova
Институт фундаментальных проблем биологии РАН, г. Пущино
Russian Federation
Russian Federation
S. V. Prokhorenko
Московская медицинская академия им. И.М. Сеченова, Москва
Russian Federation
Russian Federation
N. I. Kosjakova
ПНЦ РАН, г. Пущино
Russian Federation
Russian Federation
I. R. Prokhorenko
Институт фундаментальных проблем биологии РАН, г. Пущино;
Московская медицинская академия им. И.М. Сеченова, Москва
Russian Federation
Russian Federation
References
1. Alexander C., Rietschel E.T. Bacterial lipopolysaccharides and innate immunity // J. Endotoxin Res. – 2001. – Vol. 7. – P. 167-202.
2. Beutler B., Hoebe K., Du X., Ulevitch R.J. How we detect microbes and respond to them: the Toll-like receptors and their transducers // J. Leukoc. Biol. – 2003. – Vol. 74. – P. 479-485.
3. Galanos C., Luderitz O., Rietschel E.T. In Lipopolysaccharide: properties of an amphipathic molecule // Ed., Elsevier, Amsterdam. – 1984. – Vol. 1. – P. 46.
4. Hamann L., Alexander C., Stamme C., Zahringer U., Schumann R.R. Acute-phase concentrations of lipopolysaccharide (LPS)-binding protein inhibit innate immune cell activation by different LPS chemotypes via different mechanisms // Infection and Immunity. – 2005. – Vol. 73, N 1. – P. 193-200.
5. Jiang Z., Georgel P., Du X., Shamel L., Sovath S., Mudd S., Huber M., Kalis C., Keck S., Galanos C., Freudenberg M., Beutler B. CD14 is required for MyD88-independent LPS signaling // Nat. Immunol. – 2005. – Vol. 6, N 6. – P. 565-570.
6. Krauss J.H., Seydel U., Weckesser J., Mayer H. Structural analisis of the nontoxic lipid A of Rhodobacter capsulatus 37b4. // Eur. J. Biochem. – 1989. – Vol. 180. – P. 519-526.
7. Miyake K. Endotoxin recognition molecules, Toll-like receptor 4-MD-2. // Seminars in Immunology. – 2004.– Vol. 16, N 1. – P. 11-16.
8. Muroi M., Tanamoto K. The polysaccharide portion plays an indispensable role in Salmonella lipopolysaccharide-induced activation of NF-{kappa} B through human Toll-like receptor 4 // Infect. Immun. – 2002. –Vol. 70, N 11. – P. 6043-6047.
9. Oshiumi H., Matsumoto M., Funami K., Akazawa T., Seya T. TICAM-1, an adaptor molecule that participates in Toll-like receptor 3-mediated interferon-beta induction // Nat. Immunol. – 2003. – Vol. 4. – P. 161-167.
10. Oshiumi H., Sasai M., Shida K., Fujita T., Matsumoto M., Seya T. TIR-containing adapter molecule (TICAM)-2, a bridging adapter recruiting to toll-like receptor 4 TICAM-1 that induces interferon-beta // J. Biol. Chem. – 2003. – Vol. 278. – P. 49751-49762.
11. Poltorak A., Ricciardi-Castagnoli P., Citterio S., Beutler B. Physical contact between lipopolysaccharide and Toll-like receptor 4 revealed by genetic complementation // Proc. Natl. Acad. Sci. USA. – 2000. – Vol. 97. – P. 2163-2167.
12. Rosengart M. R., Arbabi S., Bauer G.J., Garcia I., Jelacic S., Maier R.V. The actin cytoskeleton: an essential component for enhanced TNF alpha production by adherent monocytes // Shock. – 2002. – Vol. 17, N 2. – P. 109-113.
13. Schwende H., Fitzke E., Ambs P., Dieter P. Differences in the state of differentiation of THP-1 cells induced by phorbol ester and 1,25-dihydroxyvitamin D3 // J. Leukoc. Biol. – 1996. – Vol. 59, N 4. – P. 555 - 561.
14. Shiba T., Kusumoto S., Inage M., Chaki H., Shimamoto T. Recent developments in the organic synthesis of lipid A in relation to biologic activities // Res. Infect. Dis. – 1984. – Vol. 6. – P. 478-482.
15. Suda Y., Aoyama K., Arimoto K., Tamura T., Shoichi Kusumoto S. S-form lipopolysaccharide (LPS), but not lipid A or R-chemo-type LPS, induces interleukin-6 production in vitamin D3-differentiated THP-1 cells // Biochemical and Biophysical Research Communications. – 1999. – Vol. 257, N 2. – P. 327 - 332.
16. Suda Y., Aoyama K., Arimoto K., Tamura T., Shoichi Kusumoto S. S-form lipopolysaccharide (LPS), but not lipid A or R-chemo-type LPS, induces interleukin-6 production in vitamin D3-differentiated THP-1 cells // Biochemical and Biophysical Research Communications. – 1999. –Vol. 257, N 2. – P. 327- 332.
17. Takashiba S., Shapira L., Amar S., Van Dyke T.E. Cloning and characterization of human TNF alpha promoter region // Gene. – 1993. – Vol. 131. – P. 307-308.
18. Takashiba S., Van Dyke T.E., Amar S., Murayama Y., Soskolne A.W., Shapira L. Differentiation of monocytes to macrophages primes cells for lipopolysaccharide stimulation via accumulation of cytoplasmic nuclear factor B // Infect. Immun. – 1999. – Vol. 67, N 11. – P. 5573-5578.
19. Tanamoto K.-i., Azumi S. Salmonellatype heptaacylated lipid A is inactive and acts as an antagonist of lipopolysaccharide action on human line cells. // J. Immunol. – 2000. – Vol. 164. – P. 3149 - 3156.
20. Tapping R.I., Akashi S., Miyake K., Godowski P.J., Tobias P.S. Toll-like receptor 4, but not Toll-like receptor 2, is a signaling receptor for Escherichia and Salmonella lipopolysaccharides // J. Immunol. – 2000. – Vol. 165. – P. 5780-5787.
21. Tsai C.M. and Frasch C.E. A sensitive silver stain for detecting liupopolysaccharides in polyacrylamide gels // Anal. Biochem. – 1982. – Vol. 119. – P. 115 - 119.
22. Wright S.D., Ramos R.A., Tobias P.S., Ulevitch R.J., Mathison J.C. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein // Science. – 1990. – Vol. 249. – P. 1431 - 1432.
23. Zarember K.A., Godowski P.J. Tissue expression of human Toll-like receptors and differential regulation of Toll-like receptor mRNAs in leucocytes in response to microbes, their producs, and cytokines // J. Immunol. – 2002. – Vol. 168. – P. 554-561.
24. Zughaier S.M., Tzeng Y.L., Zimmer S.M., Datta A., Carlson R.W., Stephens D.S. Neisseria meningitidis lipooligosaccharide structure-dependent activation of the macrophage CD14/Toll-like receptor 4 pathway // Infection and Immunity. – 2004. – Vol. 72, N 1. – P. 371-380.
25. Zughaier S.M., Zimmer S.M., Datta A., Carlson R.W., Stephens D.S. Differential induction of the Toll-like receptor 4-MyD88-dependent and -independent signaling pathways by endotoxins // Infect. Immun. – 2005. – Vol. 73, N 5. – P. 2940- 2950.
Review
For citations:
Voloshina E.V., Zubova S.V., Prokhorenko S.V., Kosjakova N.I., Prokhorenko I.R. A COMPARISON OF DIFFERENT LIPOPOLYSACCHARIDE CHEMOTYPES FROM ESCHERICHIA COLI AND SALMONELLA UPON SYNTHESIS OF TNFα AND IL-6 BY MACROPHAGE-LIKE THP-1 CELLS. Medical Immunology (Russia). 2009;11(6):509-514. (In Russ.) https://doi.org/10.15789/1563-0625-2009-6-509-514
Views:
1016
Email this article 