CLONING, EXPRESSION AND STLJDY OF IMMIJNOLOGICAL PROPERTIES OF THE STREPTOCOCCI GROUP B C5A PEPTIDASE RECOMBINANT PROTEIN
https://doi.org/10.15789/1563-0625-2009-1-7-14
Abstract
Molecular mass of SCPB1 was estimated to be equal to 43.0±0.5 kDa. Immunization experiments were performed in laboratory animals to investigate the SCPB1 immunogenic properties. Maximum titers of IgG antibodies against SCPB1 (1:5,1 х 104 и 1:1,6× x 106 for mice and rabbits, respectively) indicated to SCPB1 immunogenicity. Moreover, the antibodies against SCPB1 were revealed in sera from women infected with GBS thus designating the humoral immune response of human organism to C5a peptidase fragment under study. Protective properties of anti-SCPB1 antibodies against were demonstrated by experiments in vitro (opsonophagocytosis), and in vivo (a model of GBS infection in mice).
About the Authors
N. V. Duplik
Государственное учреждение «Научно-исследовательский институт экспериментальной медицины РАМН»,
Санкт-Петербург
Russian Federation
Russian Federation
I. V. Koroleva
Государственное учреждение «Научно-исследовательский институт экспериментальной медицины РАМН»,
Санкт-Петербург
Russian Federation
Russian Federation
A. N. Suvorov
Государственное учреждение «Научно-исследовательский институт экспериментальной медицины РАМН»,
Санкт-Петербург
Russian Federation
Russian Federation
References
1. Грабовская К.Б., Леонтьева Г.Ф., Мерингова Л.Ф., Воробьева Е.И., Суворов А.Н., Тотолян А.А.Протективные свойства некоторых поверхностных рекомбинантных полипептидов стрептококков группы В//ЖМЭИ. -2007. -№ 5. -С. 44-51.
2. Мерингова Л.Ф., Войцеховский Б.Л., Духин А.И., Крамская Т.А., Поляк Р.Я. Применение методов химической кинетики для оценки аффинитета циркулирующих свободных и связанных с антигеном антител (модель экспериментальный грипп)//Иммунология. -1986. -№ 6. -С. 48-51.
3. Baker C.J., Rench M.A., Edwards M.S. Immunization of pregnant woman with a polysaccharide vaccine of Group B streptococci//N. Engl. J. Med. -1988. -Vol. 319, N 18. -Р. 1180-1185.
4. Beckmann C., Waggoner J.D., Harris T.O., Tamura G.S., Rubens C.E. Identification of novel adhesins from group B streptococci by use of phage display reveals that C5a peptidase mediates fibronectin binding//Infect. Immun. -2002. -Vol. 70, N 6. -Р. 2869-2876.
5. Bohnsack J.F., Chang J.K., Hill H.R. Restricted ability of Group B Streptococcal C5a-asa to inactivate C5a prepared from different animal species//Infect. Immun. -1993. -Vol. 61. -N 4. -P. 1421-1426.
6. Chen C.C., Cleary P.P. Complete nucleotide sequence of the streptococcal C5a peptidase gene of Streptococcus pyogenes//J. Biol. Chem. -1990. -Vol. 265, N 6. -Р. 3161-3167.
7. Cheng Q., Debol S., Lam H., Eby R., Edwards L., Matsuka Y., Olmsted S., Cleary P. Immunization with C5a Peptidase or Peptidase-Type III Polysaccharide Conjugate Vaccines Enhances Clearance of Group B Streptococci from Lungs of Infected Mice//Infect. Immun. -2002. -Vol. 70, N 11. -Р. 6409-6415.
8. Cheng Q., Stafslien D., Purushothaman S.S., Cleary P. The group B streptococcal C5a peptidase is both a specific protease and an invasion//Infect. Immun. -2002. -Vol. 70, N 5. -P. 2408-2413.
9. Cheng, Q., Carlson B., Pillai S., Eby R., Edwards L., Olmsted S.B., Cleary P. Antibody against surface-bound C5a peptidase is opsonic and initiates macrophage killing of group B streptococci//Infect. Immun. -2001. -Vol. 69, N 4. -Р. 2302-2308.
10. Chmourigina Suvorov A.N., Ferrieri P., Cleary P.P. Conservation of the C5a peptidase genes in group A and B streptococci//Infect. Immun. -1996. -Vol. 67, N 7. -P. 2387-2390.
Review
For citations:
Duplik N.V., Koroleva I.V., Suvorov A.N. CLONING, EXPRESSION AND STLJDY OF IMMIJNOLOGICAL PROPERTIES OF THE STREPTOCOCCI GROUP B C5A PEPTIDASE RECOMBINANT PROTEIN. Medical Immunology (Russia). 2009;11(1):7-14. (In Russ.) https://doi.org/10.15789/1563-0625-2009-1-7-14
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