THE EFFECT OF SPLEEN LYMPHOCYTES TREATED WITH AN ORIGINAL ANTICONVULSANT ON HEMATOPOIESIS DURING LONG-TERM ALCOHOL CONSUMPTION

Abstract

Excessive alcohol consumption has a negative effect on hematopoiesis, which is expressed in a significant suppression of both the production of blood cells and structural changes in precursors, namely in the suppression of their maturation, up to pancytopenia. A distinction is made between the direct effect of alcohol (toxic effect on bone marrow, hematopoietic precursors and mature blood cells) and the indirect effect due to a deficiency of trophic factors. Alcoholics often exhibit anemia, as a consequence of the destruction of erythroid cells before they mature, and thrombocytopenia, which causes the appearance of petechiae and spontaneous bleeding. Chronic alcohol consumption also has a suppressive effect on the production and function of white blood cells, resulting in a poor ability to resist bacterial infection. We have previously identified the immunomodulatory properties of the innovative anticonvulsant meta-chlorobenzohydrylurea and demonstrated the positive psychoneuromodulatory effect of splenic lymphocytes modulated in vitro by the indicated anticonvulsant during chronic ethanol intoxication. In this study the influence of meta-chlorobenzhydrylurea-modulated spleen lymphocytes on bone marrow hematopoiesis and peripheral blood cells long-term alcoholized mice was studied. In the bone marrow of long-term alcoholized mice a decrease in the colony-forming activity of hematopoietic precursors was observed: the population of erythroid precursors was significantly reduced, and a decrease in the population of granulocyte-macrophage precursors was also recorded at a trend level. In peripheral blood, a decrease in the number of lymphocytes, platelets, erythrocytes and leukocytes was observed with an increase in the population of segmented neutrophils, indicating peripheral inflammation.     Lymphocytes precultured with meta-chlorobenzhydryl urea, after intravenous administration to syngeneic long-term alcoholized recipients, had a corrective effect on a number of hematopoietic parameters, which was manifested in the restoration of the colony-forming activity of bone marrow hematopoietic precursors to indicators comparable to those in intact mice of the corresponding age, in a decrease of segmented neutrophils and restoration of erythrocytes and lymphocytes populations with tendency to increase the number of platelets in the peripheral blood. The data obtained may indicate the effectiveness of meta-chlorobenzhydrylurea-modulated lymphocytes in correcting a number of changes in hematopoiesis provoked by long-term ethanol intoxication.

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