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Innate immune factor gene expression profiles in patients with atopic dermatitis

https://doi.org/10.15789/1563-0625-IIF-2766

Abstract

Atopic dermatitis is a multifactorial genetically determined inflammatory skin disease characterized by itching, chronically relapsing dermatitis, age-related features of localization and morphology of lesions. The pathogenesis of atopic dermatitis is complex and includes epigenetic alterations, involved in the genomic adaptation, immune response reactions and dysfunction of the epithelial barrier that together trigger the development of atopic dermatitis. The aim of this study is to detect the expression level for IL4, IL13, IL33, TLR2, TLR9 genes in the biological materials of atopic patients.

The targeted genes for further expression evaluation were selected according to our previous findings on genome-wide methylation study. We detected the cascades with the differentially methylated genes that are most likely to take place in atopic dermatitis. Thus, we investigated expression levels for the IL4, IL13, IL33, TLR2, TLR9 genes in the skin, peripheral blood mononuclear cells and whole blood cells using RT-PCR on 55 pediatric patients and 26 healthy volunteers, and on 50 adult patients. Statistical analysis was performed with the use of Kruskal-Wallis H test and Mann-Whitney U test. Targeted expression analysis revealed that in the skin samples the expression of TLR9 and IL4 was 12 times significantly lower (p < 0.0001, p < 0.0005) in the lesional skin; and there was a 6-fold decrease in case of TLR2 (p < 0.01). The results for blood mononuclear cells differed and expression levels for most of the assessed targets were significantly higher before treatment. We have also found out that those differences were strongly pronounced especially in an elder age group (12-18 y.o.). Studying the IL33 gene expression in the whole blood samples of adults revealed that its level was significantly higher in case of patients with moderate form of AD. Besides, we concluded that locally in the affected skin inflammatory immune response may dominate; in the mononuclear cells Th2 immune response apparently takes place. New insights on immunological markers and links among them may shed a light on atopic dermatitis pathogenic mechanisms. The detected molecules could play role as potential therapeutic targets and form a management approach for patients with atopic dermatitis.

About the Authors

E. P. Bystritskaia
I. Mechnikov Research Institute for Vaccines and Sera
Russian Federation

Elizaveta P. Bystritskaya - Junior Research Associate, Laboratory of Molecular Immunology, I. Mechnikov Research Institute for Vaccines and Sera.

5a Maly Kazenny Lane Moscow 105064

Phone: +7 (915) 155-86-18


Competing Interests:

None



N. N. Murashkin
Medical Research Center for Children's Health; I. Sechenov First Moscow State Medical University
Russian Federation

Nikolay N. Murashkin - PhD, MD (Medicine), Professor, Senior Research Associate, Head of the Department of Dermatology, Head of the Laboratory of Skin Pathology in Children, Medical Research Center for Children's Health; Professor, Department of Pediatrics and Pediatric Rheumatology, I. Sechenov First Moscow State Medical University.

Moscow


Competing Interests:

None



O. Yu. Olisova
I. Sechenov First Moscow State Medical University
Russian Federation

Olga Yu. Olisova - PhD, MD (Medicine), Professor, Corresponding Member, Russian Academy of Sciences, Head, V. Rakhmanov Department of Skin and Venereal Diseases, I. Sechenov First Moscow State Medical University

Moscow


Competing Interests:

None



A. I. Materikin
Medical Research Center for Children's Health
Russian Federation

Alexander I. Materikin - PhD (Medicine), Dermatovenereologist, Department of Dermatology, Medical Research Center for Children's Health.

Moscow


Competing Interests:

None



M. B. Potapova
I. Mechnikov Research Institute for Vaccines and Sera; I. Sechenov First Moscow State Medical University
Russian Federation

Mariia B. Potapova - Junior Research Associate, Laboratory of Molecular Immunology, I. Mechnikov Research Institute for Vaccines and Sera; Postgraduate Student, I. Sechenov First Moscow State Medical University.

Moscow


Competing Interests:

None



A. B. Vinnitskaya
I. Mechnikov Research Institute for Vaccines and Sera
Russian Federation

Alexandra B. Vinnitskaya - PhD (Medicine), Research Associate, Laboratory of Molecular Immunology, I. Mechnikov Research Institute for Vaccines and Sera.

Moscow


Competing Interests:

None



A. G. Upatova
I. Mechnikov Research Institute for Vaccines and Sera
Russian Federation

Laboratory Assistant, Laboratory of Molecular Immunology, I. Mechnikov Research Institute for Vaccines and Sera.

Moscow


Competing Interests:

None



References

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For citations:


Bystritskaia E.P., Murashkin N.N., Olisova O.Yu., Materikin A.I., Potapova M.B., Vinnitskaya A.B., Upatova A.G. Innate immune factor gene expression profiles in patients with atopic dermatitis. Medical Immunology (Russia). 2023;25(5):1037-1042. https://doi.org/10.15789/1563-0625-IIF-2766

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ISSN 1563-0625 (Print)
ISSN 2313-741X (Online)