INTERACTION OF TRANSCRIPTION FACTORS PAX-5 AND STAT6 IN PATHOGENESIS OF ALLERGIC BRONCHIAL ASTHMA
https://doi.org/10.15789/1563-0625-2014-1-35-42
Abstract
The aim of this work was to study an opportunity of coordinated expression of PAX-5 and STAT6 in bronchial asthma (BA) development. 35 healthy controls, 83 patients with allergic bronchial asthma (ABA) and 63 patients with nonallergic bronchial asthma (NABA) o different severity were observed. To evaluate PAX- 5 and β-actin mRNA levels, a reverse transcription-PCR with endpoint detection was applied. To determine STAT6 and pSTAT6 protein levels, ECL Western blotting protocols (Amersham) was used. A negative correlation was revealed between РАХ-5 and STAT6 mRNA expression levels in control group and in ABA. In NABA patients, the appropriate correlations were nonsignificant. Expression of РАХ-5 mRNA in ABA with normal IgE levels showed a significant negative correlation with that of STAT6. Meanwhile, expression levels of active pSTAT6 form in patients with high IgE and eosinophilia did negatively correlate with РАХ-5 mRNA expression. In ABA, effects of IL-4 caused a more significant decrease of РАХ-5 mRNA as compared with those in control group. The strongest negative correlation between РАХ-5 mRNA expression and STAT6 levels was revealed in groups with higher levels of mRNA РАХ-5 and lower levels of STAT6. The revealed negative correlations between STAT6 levels and РАХ-5 mRNA expression suggest that STAT6 may inhibit PAX-5 transcription in ABA group, thus, probably, promoting differentiation of B cells into plasma cells.
Supporting agencies: St. Petersburg Government
About the Authors
V. N. Mineev
St. Petersburg State I.P. Pavlov Medical University, St. Petersburg
Russian Federation
PhD, MD (Medicine), Professor,
M.V. Chernorutsky Department of Hospital Therapy
Russian Federation
PhD, MD (Medicine), Professor,
M.V. Chernorutsky Department of Hospital Therapy
L. N. Sorokina
St. Petersburg State I.P. Pavlov Medical University, St. Petersburg
Russian Federation
Russian Federation
PhD, MD (Medicine), Associate
Professor, M.V. Chernorutsky Department of Hospital
Therapy
M. A. Nyoma
St. Petersburg State I.P. Pavlov Medical University, St. Petersburg
Russian Federation
Russian Federation
PhD Candidate, M.V. Chernorutsky
Department of Hospital Therapy
A. V. Eremeeva
St. Petersburg State I.P. Pavlov Medical University, St. Petersburg
Russian Federation
Russian Federation
PhD Candidate, M.V. Chernorutsky
Department of Hospital Therapy
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For citations:
Mineev V.N., Sorokina L.N., Nyoma M.A., Eremeeva A.V. INTERACTION OF TRANSCRIPTION FACTORS PAX-5 AND STAT6 IN PATHOGENESIS OF ALLERGIC BRONCHIAL ASTHMA. Medical Immunology (Russia). 2014;16(1):35-42. (In Russ.) https://doi.org/10.15789/1563-0625-2014-1-35-42
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