FLOW CYTOMETRY AS A MODERN ANALYTICAL TOOL IN BIOLOGY AND MEDICINE
https://doi.org/10.15789/1563-0625-2007-4-5-
Abstract
Abstract. Flow cytometry is considered as a modern technology for fast measurements of cellular characteristics, their organelles, and processes occurring within them. It is regarded as an efficient solution in many important areas of cell biology, immunology and cellular engineering. Present article bears on main developments in flow cytometry and their applications in medical and biological practice. Usage of modern achievements in fluorescent dyes, progress in laser and computer technologies, as well as potent software, resulted in wide application of this technique in medical practice. Accordingly, usage of monoclonal antibodies conjugated to different fluorochromes has led to elaboration of multiparametric analysis and did sufficiently simplify specialized works aimed for diagnostics of various immune disorders. The new directions in flow cytometry, e.g., flow cytoenzymology, provide wide opportunities for detailed identification of damaged or altered cells, and taking adequate decisions in treatment of detected pathological changes. The authors suggest that this article could initiate a series of publications concerning usage of this technology and its modern applications in broad laboratory practice.
About the Authors
S. V. Khaidukov
Институт Биоорганической химии им. акад. М.М. Шемякина и Ю.А. Овчинникова РАН, Москва
Russian Federation
Russian Federation
A. V. Zurochka
ГОУ ВПО Челябинская государственная медицинская академия РОСЗДРАВА, г. Челябинск
Russian Federation
Russian Federation
References
1. Луговская С.А., Почтарь М.Е., Тупицын Н.Н. Иммунофенотипирование в диагностике гемобластозов. – М., Тверь: Триада, 2005. – 168 с.
2. Полетаев А.И. Проточная цитометрия и сортировка в цитологии, молекулярной биологии, биотехнологии и медицине / ИНТ серия «Общие проблемы физико-химической биологии». – М., 1989. – Т. 12. – 88 с.
3. Шмаров Д.А., Козинец Г.И. Лабораторно-клиническое значение проточно-цитометрического анализа крови. – М.: МИА, 2004. – 128 с.
4. Bossuyt X., Marti G.E., Fleisher T.A. Comparative analysis of whole blood lysis methods for flow cytometry // Cytometry. – 1997. – Vol. 30. – P. 124-133.
5. Chou T., Sano M., Ogura M., Marishima Y., Itagaki H., Takuda Y. Isolation and transplantation of highly purified autologous peripheral CD34+ progenitor cells: purging efficacy, hematopoietic reconstitution following high dose chemotherapy in patients with breast cancer: results of feasibility study in Japan // Brest Cancer. – 2005. – Vol. 12, N 3. – P. 178-188.
6. Claude L., Lobagiu C., Genin C. Enumeration of peripheral lymphocyte subsets using 6 vs. 4 color staining: a clinical evaluation of new flowcytometer // Cytometry, Part B. – 2005. – Vol. 70B. – P. 29-38.
7. Comans-Bitter W.M., de Groot R., van den Beemd R., Neijens H.J., Hop W.C., Groeneveld K., Hooijkaas H., van Dongen J.J. Immunophenotyping of blood lymphocytes in childhood. Reference values for lymphocyte subpopulations // J. Pediatr. – 1997. – Vol. 130, N 3. – P. 388-393.
8. Di Nicola M., Siena S., Bregni M., Peccatori F., Magni M., Ravagnani F., Zorzino L., Bonadonna G., Gianni A.M. Quantization of CD34+ peripheral blood hematopoietic progenitors for autografting in cancer patients // Int. J. Artif. Organs. – 1993. – Suppl. 5. – P. 80-82.
9. Llorente L., De La Fuente H., Richaud-Patin Y., Alvarado-De La Barrera C., Diaz-Borjon A., Lopez- Ponce A., Lerman-Garber I., Jakez-Ocampo J. Innate immune response mechanisms in non-insulin dependent diabetes mellitus patients assessed by flow cytoenzymology // Immunol. Lett. – 2000. – Vol. 74, N 3. – P. 239-244.
10. Leuсocyte typing VI / Ed. Kishimoto K. et al. – Garland Publishing, Inc. N. Y. & L., 1997. – P. 1342.
11. Luider J., Cyfra M., Johnson P., Auer I. Impact of the new Beckman Coulter Cytomics FC 500 5-color flow cytometer on a regional flow cytometry clinical laboratory servise // Lab. Hematol. – 2004. – Vol. 10. – P. 102-108.
12. Pope V., Larsen S.A., Rice R.J., Goforth S.N., Parham C.E., Fears M.B. Flow cytometric analysis of peripheral blood lymphocyte immunophenotypes in persons infected with treponema pallidum // Clin. Diagn. Lab. Immunol. – 1994. – Vol. 1, N 1. – P. 121-124.
13. Rack K.A., Harris P.C., MacCarthy A.B., Boone R., Raynham H., McKinleyt M., Fitchett M., Towe C.M., Rudd P., Armour J.A.L., Lindenbaum R.H., Buckle V.J. Characterization of three de novo derivative chromosomes 16 by «reverse chromosome painting» and molecular analysis // Am. J. Hum. Genet. – 1993. – Vol. 52. – P. 987-997.
14. Sack U., Scheibe R., Wotzel M., Hammerschmidt S., Kuhn H., Emmrich F., Hoheisel G.,Wirtz H., Gessner C. Multiplex analysis of cytokines in exhaled breath condensate // Cytometry. – 2006. – Vol. 69, Iss. 3. – P. 169-172.
15. Shapiro H.M. Practical flow cytometry. – WILEY-LISS, Inc., N.Y., Chichester, Brisbane, Toronto & Singapore, 1995. – P. 542.
16. Stratieva-Taneeva P.A., Khaidukov S.V., Kovalenko V.A., Nazimov I.V., Samochvalova L.V., Nesmeyanov V.A. Bispecific monoclonal antibodies to human Interleikin 2 and horseredish peroxidase // Hybridoma. – 1993. – Vol. 12, N 3. – P. 271-284.
17. Teixeira G., Lenormand B., Jean P., Fardoun D., Sumereau-Dassin E., Bastit D., Tilly H., Piguet H., Monconduit M., Vannier J.P. Immunological evaluation of harvested stem cells obtained by leukapheresis after chemotherapy // Am. J. Hematol. – 1992. – Vol. 39, N 3. – P. 172-175.
18. Xian H.-Q., McNichols E., St. Clair A., Gottlieb D.I. A subset of ES-cell-derived neural cells marked by gene targeting // Stem Cells. – 2003. – Vol. 21. – P. 41-49.
19. Xu L., Zha Q., Sun H., Jia Q., Li F., He X. Preparation and characterization of HLA-A*0201 tetramer loaded with IE-1316-324 antigenic peptide of human cytomegalovirus // Cell Mol. Immunol. – 2006. – Vol. 3, N 5. – P. 367-371.
20. Zidovec Lepej S., Vince A., Raku ic S., Рakovic Rode O., Sonicki Z., Jeren T. Center for disease control (CDC) flow cytometry panel for human immunodeficiency virus infection allows recognition of infectious mononucleosis caused by Epstein–Barr virus or Cytomegalovirus // Croat. Med. J. – 2003. – Vol. 44. – P. 702-706.
Review
For citations:
Khaidukov S.V., Zurochka A.V. FLOW CYTOMETRY AS A MODERN ANALYTICAL TOOL IN BIOLOGY AND MEDICINE. Medical Immunology (Russia). 2007;9(4-5):373-378. (In Russ.) https://doi.org/10.15789/1563-0625-2007-4-5-
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