ASSOCIATION OF MATRIX METALLOPROTEINASES GENE POLYMORPHISM WITH CLINICAL MANIFESTATIONS OF BRONCHIAL ASTHMA IN CHILDREN
https://doi.org/10.15789/1563-0625-2018-6-905-912
Abstract
In the present study, we have examined association between different polymorphic variants of metalloproteinases genes and clinical manifestations of bronchial asthma in children. We observed 103 patients including 42 children with an established diagnosis of asthma. Moreover, 61 persons were examined in the control group. All patients underwent genetic testing by allele-specific polymerase chain reaction. In particular, 320A>C polymorphic locus of ММР20 gene; Val275Ala ММР20, and -8202A>G gene ММР9 were analyzed.
We have found that 30 patients (71.4% of total) had bronchial asthma of mild severity, 9 children (21.4%) exhibited moderate degree, and 3 patients (7%) had severe-grade disease. Homozygous C/C variant of the polymorphic ММР20 gene, 320A>C heterozygous variant of the ММР20 Val275Ala polymorphism, and heterozygous locus of -8202A>G ММР9 gene were found to be most frequent among the children with asthma. Generally, we have observed that the frequencies of the studied alleles and genotypes did not significantly differ berween the asthma patients and children from the control group (p < 0.05). However, in patients with GGgenotype of -8202A>G ММР9 polymorphism combined with homozygosity for the C allele of ММР20 320A>C, a more severe disease was observed, being combined with polyvalent sensitization and high total IgE levels in blood serum.
In conclusion, frequencies of alleles and genotypes among patients with asthma did not show any statistically significant differences from the group of healthy children. The patients homozygous for G allele of ММР9 -8202A>G polymorphism gene and for the C allele ММР20 gene (320A>C) seem to be predisposed for a more severe clinical course of the disease.
About the Authors
A. A. Lebedenko
Rostov State Medical University
Russian Federation
Russian Federation
PhD, MD (Medicine), Profesor, Head, Department of Children’s Diseases No. 2
T. P. Shkurat
Southern Federal University
Russian Federation
Russian Federation
PhD, MD (Biology), Professor, Head, Department of Genetics
E. V. Mashkina
Southern Federal University
Russian Federation
Russian Federation
PhD, MD (Biology), Associate Professor, Department of Genetics
O. E. Semernik
Rostov State Medical University
Russian Federation
Russian Federation
PhD (Medicine), Assistant Professor, Department of Children’s Diseases No. 2
344022, Russian Federation, Rostov-on-Don, Pushkinskaya str., 176, apt 18.
Phone: 7 (918) 569-26-81
T. K. Dreyzina
ФГАОУ ВО «Южный федеральный университет»
Russian Federation
Student, Department of Genetics
Russian Federation
Student, Department of Genetics
E. B. Tyurina
Rostov State Medical University
Russian Federation
Clinical Pediatrician, Pediatric Clinic
Russian Federation
Clinical Pediatrician, Pediatric Clinic
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Review
For citations:
Lebedenko A.A., Shkurat T.P., Mashkina E.V., Semernik O.E., Dreyzina T.K., Tyurina E.B. ASSOCIATION OF MATRIX METALLOPROTEINASES GENE POLYMORPHISM WITH CLINICAL MANIFESTATIONS OF BRONCHIAL ASTHMA IN CHILDREN. Medical Immunology (Russia). 2018;20(6):905-912. (In Russ.) https://doi.org/10.15789/1563-0625-2018-6-905-912
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