SERUM LEVEL AND GENE POLYMORPHISM OF INTERLEUKIN-1α, AND EFFICIENCY OF INFERTILITY TREATMENT BY IN VITRO FERTILIZATION

Search for novel prognostic criteria predicting successful in vitro fertilization remains a nonresolved problem at the present time. The aim of our study was to analyse a predictive role of IL-1α as an additional marker of pregnancy after in vitro fertilization (IVF). The study included 120 women with tubo-peritoneal infertility subjected to the IVF procedure. Retrospectively, two groups were formed of this cohort, dependent on efficiency of in vitro fertilization. Group I included 40 women with successful pregnancy whereas group II comprised 80 women with failed pregnancy. IL-1α concentrations in serum were detected by ELISA technique. A polymorphic rs1800587 marker at 5`UTR region has been amplified by PCR followed by Sanger sequencing. We have shown IL-1α hyperproduction in the women from group I. The women with effective IVF outcome exhibited positive correlation between IL-1α and luteinizing hormone, prolactin, progesterone, 17-hydroxyprogesterone, dehydroepiandrosterone sulfate levels. The women with ineffective in vitro fertilization have detected a negative correlation between IL-1α levels and anti-Muellerian hormone, a positive correlation of IL-1α with 17-hydroxyprogesterone. The women with T allele of the polymorphic rs1800587 marker at 5`UTR region have shown a 2.5-fold higher chance to become pregnant after IVF than the women carrying C allele (95% CI = [1.45-4.35], р = 0.0009). The women with T/T genotype exhibited a positive correlation between IL-1α and estradiol, testosterone; the subjects with heterozygous C/T genotype showed correlation with estradiol, and those harboring C/C genotype exhibited correlation with follicle-stimulating hormone. The revealed changes suggest a potential involvement of IL-1α into regulation of cyclic processes in the ovary including ovulation. Moreover, IL-1α may participate in formation of pro-inflammatory environment for successful blastocyst implantation.

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