STUDY OF APOPTOSIS OF IMMUNE CELLS IN TYPE 1 DIABETES MELLITUS
https://doi.org/10.15789/1563-0625-2017-5-635-640
Abstract
Type 1 diabetic mellitus (T1DM) is known to be associated with progressive destruction of β-cells of the pancreas. Dysregulated immunity and programmed cell death are an important link in pathogenesis of diabetes. In this study, we examined expression levels of interleukin-2, Bcl-1, ANXA-11 genes in patients with T1DM. The study was done with blood leukocures of T1DM patients (30) and healthy controls (70). Reverse-transcription PCR was done with Transcriptor First Strand cDNA Synthesis Kit from Roche Life Science. The study of IL-2 and Bcl-1 gene expression level in peripheral blood leukocytes indicated that the median gene expression levels of IL-2 and Bcl-1 are increased in patients with T1DM patients compared with control group of healthy persons. The study of apoptosis by annexin test has revealed an increased level of ANXA 11 expression in T1DM patients. The obtained data can serve as an additional source for understanding the pathogenesis of T1DM mechanisms.
About the Authors
L. M. Hovsepyan
Institute of Molecular Biology, National Academy of Sciences, Republic of Armenia.
Armenia
Armenia
Hovsepyan L.M., PhD (Biology), Leading Research Associate, Chief, Laboratory of Molecular Membranology, Institute of Molecular Biology, National Academy of Sciences, Republic of Armenia.
Yerevan.
G. S. Kazaryan
Institute of Molecular Biology, National Academy of Sciences, Republic of Armenia.
Armenia
Armenia
Kazaryan G.S., PhD (Biology), Research Associate, Laboratory of Molecular Membranology, Institute of Molecular Biology, National Academy of Sciences, Republic of Armenia.
Yerevan.
A. V. Zanginyan
Institute of Molecular Biology, National Academy of Sciences, Republic of Armenia.
Armenia
Armenia
Zanginyan A.V., PhD (Biology), Research Associate, Laboratory of Molecular Membranology, Institute of Molecular Biology, National Academy of Sciences, Republic of Armenia.
Yerevan.
A. A. Akopdzhanyan
Institute of Molecular Biology, National Academy of Sciences, Republic of Armenia.
Armenia
Armenia
Akopdzhanyan A.A., PhD (Biology), Research Associate, Laboratory of Molecular Membranology, Institute of Molecular Biology, National Academy of Sciences, Republic of Armenia.
Yerevan.
G. V. Zakharyan
Institute of Molecular Biology, National Academy of Sciences, Republic of Armenia.
Armenia
Armenia
Zakharyan G.V., PhD (Biology), Research Associate, Laboratory of Molecular Membranology, Institute of Molecular Biology, National Academy of Sciences, Republic of Armenia.
Yerevan.
Martin Petrek
Palacky University.
Czech Republic
Czech Republic
Petrek Martin, Head of Laboratory, Department of Pathophysiology, Palacky University.
Olomouc.
References
1. Балаболкин М.И. Диабетология. М.: Медицина, 2004. 383 с. [Balabolkin M.I. Diabetology]. Moscow: Medicine, 2004. 383 p.
2. Бра М., Квинан Б., Сузин С.А. Митохондрии в программированной гибели клетки: различные механизмы гибели // Биохимия, 2005. Т. 70, № 2. С. 284-293. [Bra M., Kvinan B., Suzin S.A. Programmed cell death via mitochondria: Different modes of dying. Biokhimiya = Biochemistry, 2005, Vol. 70, no. 2, pp. 231-239. (In Russ.)]
3. Карян Ш.С., Овсепян, Л.М., Захарян Г.В., Карагезян К.Г. Особенности нормализующего действия лазерного излучения при нарушениях метаболизма фосфолипидов и процессов перекисеобразования у аллоксандиабетических белых крыс // Информационные технологии и управление, 2003. T. 2. C. 212-218. [Кaryan Sh.S., Hovsepyan L., Zakharyan G., Karagezyan K.G. Specifities of laser irradiation normalizing effect at disturbances of phospholipid metabolism and peroxideformation processes of alloxandiabeted rats. Informatsionnye tekhnologii i upravlenie = Informaton Technologies and Management 2003, Vol. 2, pp. 212-218. (In Russ.)]
4. Саприна Т.В., Лазаренко Ф.Э., Прохоренко Т.С., Рязанцева Н.В., Новицкий В.В. Особенности продукции интерлейкина-2 мононуклеарными лейкоцитами крови при аутоиммунном сахарном диабете // Сибирский медицинский журнал, 2011. Т. 26, № 4. C. 65-70. [Saprina T.V., Lazarenko F.E., Prokhorenko T.S., Ryazantseva N.V., Novitsky V.V. Peculiariries of production and reception features of interleukin 2 by mononuclear leukocytes in autoimmune diabetes mellitus. Sibirskiy meditsinskiy zhurnal = Siberian Medical Journal, 2011, Vol. 26, no. 4, pp. 65-70. (In Russ.)]
5. Ярилин А.А., Никонова М.Ф., Ярилина А.А., Варфоломеева М.И., Григорьева Т.Ю. Апоптоз,роль в патологии и значимость его оценки при клиникоиммунологическом обследовании больных // Медицинская иммунология, 2000. Т. 2, № 1. С. 7-16. [Yarilin A.A., Nikonova M.F., Yarilina A.A., Varfolomeeva M.I., Grigorieva T. Yu. Apoptosis, importance of its evaluation in immunopatological states. Meditsinskaya immunologiya = Medical Immunology (Russia), 2000, Vol. 2, no. 1, pp. 7-16. (In Russ.)]
6. Cnop M., Welsh N., Jonas J.C., Jörns A., Lenzen S., Eizirik D.L. Mechanisms of pancreatic beta-cell death in type 1 and type 2 diabetes: many differences, few similarities. Diabetes, 2005, Vol. 54, pp. 97-107.
7. Eguchi Katsumi, Kawakami A. Involvement of apoptotic cell death in autoimmune diseases. Medical Electron Microscopy, 2002, Vol. 35, Issue 1, pp. 1-8.
8. Fadok V., Rose D. M., Pearson A. A receptor for phosphatidylserine-specific clearance of apoptotic cells. Nature, 2000, Vol. 405, pp. 85-90.
9. Green D.R. Apoptotic pathways: ten minutes to dead. Cell, 2005, Vol. 121, pp. 671-674.
10. Hussain M.J., Maher J., Warnock Т., Vats A., Peakman M., Vergani D. Cytokine overproduction in healthy first degree relatives of patients with IDDM. Diabetologia, 1998, Vol. 41. no. 3, pp. 343-349.
11. Mull A. Reverse transcription: do the fip. Nat. Rev. Moll. Cell Biol., 2008, Vol. 9, pp. 501-505.
12. Wang J.,Guo C., Liu S., Yin Y., Liang R., Sun M.Z., Frederick G. Annexin A-11 in disease. Clin. Chim. Acta, 2014, Vol. 431, pp. 164-168.
13. Xu H., Chen Y., Li Y., Xia F., Han B., Zhang H., Zhai H., Wu H., Li Y., Lu Y. Mitochondrial apoptosis of lymphocyte is induced in type 2 diabetes. Chin. Med. J. (Engl.), 2014, Vol. 127, no. 2, pp. 213-217.
Review
For citations:
Hovsepyan L.M., Kazaryan G.S., Zanginyan A.V., Akopdzhanyan A.A., Zakharyan G.V., Petrek M. STUDY OF APOPTOSIS OF IMMUNE CELLS IN TYPE 1 DIABETES MELLITUS. Medical Immunology (Russia). 2017;19(5):635-640. (In Russ.) https://doi.org/10.15789/1563-0625-2017-5-635-640
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