REGULATION OF TLR/RLR GENE ACTIVITY AND SYNTHESIS OF CYTOKINES DURING PHORBOL MYRISTATE ACETATE (PMA)-INDUCED DIFFERENTIATION OF THP-1 MONOCYTES INTO MACROPHAGE-LIKE CELLS
https://doi.org/10.15789/1563-0625-2017-1-27-34
Abstract
The levels of TLR/RLR gene expression and production of some cytokines were studied in monocytic THP-1 cell line during its differentiation to mature macrophage-like forms induced by phorbol 12-myristate 13-acetate (PMA) treatment for 1 and 5 days in vitro. For the first time, we have shown high induction levels for the genes that encode signaling immune receptors and transcription factors in response to PMA, as well as inhibitory effects of TLR3, TLR7/TLR8, TLR9-agonists in mature macrophages. The PMAactivated THP-1 macrophage-like cells secreted large quantitities of inflammatory IL-1β and TNFα cytokines into culture medium.
About the Authors
T. M. Sokolova
N.F. Gamaleya Research Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
PhD, MD (Biology), Leading Research Associate, Laboratory of Interferonogenesis,
123098, Moscow, Gamaleya str., 18
V. V. Poloskov
N.F. Gamaleya Research Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
Junior Research Associate, Laboratory of Interferonogenesis,
Moscow
A. N. Shuvalov
N.F. Gamaleya Research Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
Junior Research Associate, Laboratory of Interferonogenesis,
Moscow
F. I. Ershov
N.F. Gamaleya Research Institute of Epidemiology and Microbiology
Russian Federation
Russian Federation
PhD, MD (Medicine), Professor, Full Member, Russian Academy of Sciences, Chief, Department of Interferons and Laboratory of Interferonogenesis,
Moscow
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For citations:
Sokolova T.M., Poloskov V.V., Shuvalov A.N., Ershov F.I. REGULATION OF TLR/RLR GENE ACTIVITY AND SYNTHESIS OF CYTOKINES DURING PHORBOL MYRISTATE ACETATE (PMA)-INDUCED DIFFERENTIATION OF THP-1 MONOCYTES INTO MACROPHAGE-LIKE CELLS. Medical Immunology (Russia). 2017;19(1):27-34. (In Russ.) https://doi.org/10.15789/1563-0625-2017-1-27-34
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