STUDIES OF INNATE IMMUNITY PARAMETERS (TLR2, TLR4 AND HBD1) OF URETHERAL MUCOUS EPITHELIUM IN CHRONIC BACTERIAL CYSTITIS
https://doi.org/10.15789/1563-0625-2016-5-469-474
Abstract
The study for quantitation of TLR2, TLR4 and HBD-1 gene expression in urethral mucosal epithelium from women with chronic bacterial cystitis and healthy females. Materials and methods: RNA was extracted from the samples of urethral mucosa followed by RT-PCR. Results: increased expression of TLR2, TLR4 (respectively, 6.9-, 2.6-fold), along with 13.6-fold decrease in HBD-1 was revealed in women with chronic bacterial cystitis, as compared with appropriate parameters in normal women. Six months later, after combined therapy with autologous immunopeptide complex, TLR2, TLR4 и HBD1 expression levels proved to be near-normal in the patients, being similar to those in healthy females. Conclusion: Imbalanced expression of genes controlling innate immunity was revealed in urethral mucosa of the patients with chronic cystitis, thus increasing risk for urinary tract infections.
About the Authors
O. A. Svitich
N. Pyrogov Russian National Research Medical University;
I. Mechnikov Research Institute of Vaccines and Sera
Russian Federation
Russian Federation
PhD, MD (Medicine), Chief, Laboratory of Molecular Immunology,
123557, Moscow, Zoologicheskii Lane, 8, 100
L. V. Gankovskaya
N. Pyrogov Russian National Research Medical University
Russian Federation
Russian Federation
PhD, MD (Medicine), Professor, Head, Department of Immunology, Faculty of Medical Biology,
Moscow
A. V. Berenshtein
N. Pyrogov Russian National Research Medical University
Russian Federation
Russian Federation
Graduate Student, Department of Immunology, Faculty of Medical Biology,
Moscow
T. S. Perepanova
A. Lopatkin Research Institute of Urology and Interventional Radiology
Russian Federation
Russian Federation
PhD, MD (Medicine), Head, Department of Inflammatory Diseases,
Moscow
E. M. Volkova
A. Lopatkin Research Institute of Urology and Interventional Radiology
Russian Federation
Russian Federation
Postgraduate Student,
Moscow
References
1. Ганковская О.А, Ковальчук Л.В., Ганковская Л.В. Роль Toll-подобных рецепторов и дефенсинов в противомикробной защите урогенитального тракта женщин // Журнал микробиологии, эпидемиологии и иммунобиологии, 2008. №1. С.46-50. [Gankovskaya O.A., Koval’chuk L.V., Gankovskaya L.V., Lavrov V.F., Romanovskaya V.V., Kartashov D.D., Fenzeleva V.A. Role of Toll-like receptors and defensins in antimicrobial protection of urogenital tract in females. Zhurnal mikrobiologii, epidemiologii i immunobiologii = Journal of Miсrobiology, Epidemiology, Immunobiology, 2008, no. 1, pp. 46-50. (In Russ.)]
2. Гланц С. Медико-биологическая статистика. М.: Практика, 1998. 459 с. [Glants S. Medical-biological statistics]. Moscow: Practice, 1998. 459 p.
3. Ковальчук Л.В., Ганковская Л.В., Мешкова Р.Я. Клиническая иммунология и аллергология с основами общей иммунологии. М.: ГЭОТАР-Медиа, 2011. 640 c. [Kovalchuk L.V., Gankovskaya L.V., Meshkov R.J. Clinical immunology and allergology with the basics of fundamental immunology]. Moscow: GEOTAR-Media, 2011. 640 p.
4. Becknell B., Spencer J.D., Carpenter A.R. Expression and antimicrobial function of beta-defensin 1 in the lower urinary tract. PLoS One, 2013, Vol. 8, no. 10, e77714.
5. Foxman B. Recurring urinary tract infection: incidence and risk factors. Am. J. Public Health,1990, Vol. 80, no. 3, pp. 331-333.
6. Hagberg L., Hull R., Hull S. Difference in susceptibility to gram-negative urinary tract infection between C3H/HeJ and C3H/HeN mice. Infect. Immun., 1984, Vol. 46, no. 3, pp. 839-844.
7. Hawn T.R., Scholes D., Li S.S. Toll-like receptor polymorphisms and susceptibility to urinary tract infections in adult women. PLoS One, 2009, Vol. 4, no. 6, e5990.
8. Hooton T.M., Scholes D., Hughes J.P. A prospective study of risk factors for symptomatic urinary tract infection in young women. N. Engl. J. Med., 1996, Vol. 335, no. 7, pp. 468-474.
9. Ikäheimo R., Siitonen A., Heiskanen T. Recurrence of urinary tract infection in a primary care setting: analysis of a 1-year follow-up of 179 women. Clin. Infect. Dis., 1996, Vol. 22, no. 1, pp. 91-99.
10. Medzhitov R., Preston-Hurlburt P., Janeway C.A.Jr. A human homologue of the Drosophila Toll protein signals activation of adaptive immunity. Nature, 1997, Vol. 388, no. 6640, pp. 394-397.
11. Samuelsson P., Hang L., Wullt B. Toll-like receptor 4 expression and cytokine responses in the human urinary tract mucosa. Infect. Immun., 2004, Vol. 72, no. 6, pp. 3179-3186.
12. Song J., Abraham S.N. Innate and adaptive immune responses in the urinary tract. Eur. J. Clin. Invest., 2008, Vol. 38, Suppl. 2, pp. 21-28.
13. Townes C.L., Ali A., Robson W. Tolerance of bacteriuria after urinary diversion is linked to antimicrobial peptide activity. Urology, 2011, Vol. 77, no. 2, p. 509, e1-8.
14. Wolfs T.G., Buurman W.A., van Schadewijk A. In vivo expression of Toll-like receptor 2 and 4 by renal epithelial cells: IFN-gamma and TNF-alpha mediated up-regulation during inflammation. J. Immunol., 2002, Vol. 168, no. 3, pp. 1286-1293.
15. Zasloff M. Antimicrobial peptides, innate immunity, and the normally sterile urinary tract. J. Am. Soc. Nephrol., 2007, Vol. 18, no. 11, pp. 2810-2816.
Review
For citations:
Svitich O.A., Gankovskaya L.V., Berenshtein A.V., Perepanova T.S., Volkova E.M. STUDIES OF INNATE IMMUNITY PARAMETERS (TLR2, TLR4 AND HBD1) OF URETHERAL MUCOUS EPITHELIUM IN CHRONIC BACTERIAL CYSTITIS. Medical Immunology (Russia). 2016;18(5):469-474. (In Russ.) https://doi.org/10.15789/1563-0625-2016-5-469-474
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