SUBPOPULATION PROFILES OF T HELPER CELLS EXPRESSING CD45RA AND CD31 MARKERS IN CHILDREN AFTER THYMECTOMY PERFORMED UPON SURGICAL TREATMENT OF CONGENITAL HEART DISEASE
https://doi.org/10.15789/1563-0625-2016-2-119-128
Abstract
Thymectomy is a stage of surgery when treating some congenital heart defects. Thymus gland is the central organ of immune system. This organ is the primary site of T-cell lymphopoiesis and central tolerance to autoantigens during fetal and early postnatal life. If performed neonatally or in infancy, the thymectomy may cause restriction of these immune functions. Suppression of T-cell lymphopoiesis in children with thymectomy can be estimated as a subpopulation of thymic naive T helper cells (CD3+CD4+CD45RA+CD31+). To perform this task, we evaluated subpopulations of thymic naive T helper lymphocytes with CD3+CD4+CD45RA+CD31+ phenotype in the children (n = 40) who underwent thymectomy during surgical treatment of congenital heart diseases in neonates, or in early postnatal life. Their data were compared with children who underwent surgical treatment of congenital heart disease without thymectomy at the same age periods (n = 12), and healthy children (n = 23). We have revealed that thymectomy in frames of surgery of congenital heart disease leads to reduced thymic naive T helper lymphocytes with CD3+CD4+CD45RA+CD31+ phenotype in peripheral blood. Early execution of thymectomy is associated with deficiency of the thymic naive T helper lymphocytes in the peripheral blood, as well as a decrease in T helper cells (CD3+CD4+). The number thymic naive T helper lymphocytes in peripheral blood negatively corrrelated with terms elapsed after the surgery of congenital heart defects in children.
About the Authors
Yu. I. Rovda
Kemerovo State Medical Academy
Russian Federation
Russian Federation
PhD, MD (Medicine), Head, Department of Pediatrics and Neonatology,
Kemerovo
S. A. Shmulevich
Kemerovo Cardiology Dispensary
Russian Federation
Russian Federation
Head, Department of Pediatric Cardiology,
Kemerovo
A. V. Shabaldin
Research Institute for Complex Issues of Cardiovascular Diseases
Russian Federation
Russian Federation
PhD, MD (Medicine), Leading Research Associate, Laboratory of Cellular Technologies,
652002, Kemerovo, Sosnovy blvd, 6
E. B. Lukoyanycheva
Kemerovo Regional Hospital
Russian Federation
Russian Federation
Head, Immunology Laboratory,
Kemerovo
References
1. Кудрявцев И.В. Т-клетки памяти: основные популяции и стадии дифференцировки // Российский иммунологический журнал, 2014. Т. 8, № 17. С. 947-964. [Kudryavtsev I.V. T-memory cells: basic population and stage of differentiation. Rossiyskiy immunologicheskiy zhurnal = Russian Journal of Immunology, 2014, Vol. 8, no. 17, pp. 947-964. (In Russ.)]
2. Brearley S., Gentle T.A., Baynham M.I., Roberts K.D., Abrams L.D., Thompson R.A. Immunodeficiency following neonatal thymectomy in man. Clin. Exp. Immunol., 1997, Vol. 70, pp. 322-327.
3. Fry T.J., Mackall C.L. Current concepts of thymic aging. Springer Semin. Immunopathol., 2002, Vol. 24, pp. 7-22.
4. Halnon N.J., Jamieson B., Plunkett M., Kitchen C.M., Pham T., Krogstad P. Thymic function and impaired maintenance of peripheral T-cell populations in children with congenital heart disease and surgical thymectomy. Pediatr. Res., 2005, Vol. 57, pp. 42-48.
5. Jamieson B.D. Generation of functional thymocytes in the human adult. Immunity, 1999, Vol.10, pp. 569-575.
6. Junge S., Kloeckener-Gruissem B., Zufferey R., Keisker A., Salgo B. Correlation between recent thymic emigrants and CD31+ (PECAM-1) CD4+ T cells in normal individuals during aging and in lymphopenic children. Eur. J. Immunol., 2007, Vol. 37, pp. 3270-3280.
7. Kimmig S., Przybylski G.K., Schmidt C.A., Laurisch K., Mowes B. Two subsets of naive T helper cells with distinct T cell receptor excision circle content in human adult peripheral blood. J. Exp. Med., 2002, Vol. 195, pp. 789-794.
8. Kohler S., Thiel A. Life after the thymus: CD31+ and CD31- human naive CD4+ T-cell subsets. Blood, 2009, Vol. 113, pp. 769-774.
9. Madhok A.B., Chandrasekran A., Parnell V., Gandhi M., Chowdhury D., Pahwa S. Levels of Recent Thymic Emigrant Cells Decrease in Children Undergoing Partial Thymectomy during Cardiac Surgery. Clin. Diagn. Lab. Immunol., 2005, Vol. 12, no. 5, pp. 563-565.
10. Marelli-Berg F.M., Clement M., Mauro C., Caligiuri G. An immunologist’s guide to CD31 function in T-cells. J. Cell Sci., 2013, Vol. 126, pp. 2343-2352.
11. Merkenschlager M., Terry L., Edwards R., Beverley P.C. Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation. Eur. J. Immunol., 1988, Vol. 18, pp. 1653-1661.
12. Peterson P., Tõnis Org, Rebane A. Transcriptional regulation by AIRE: molecular mechanisms of central tolerance. Nature Reviews Immunology, 2008, Vol. 8, pp. 948-957.
13. Roifman C.M. Studies of patients’ thymi aid in the discovery and characterization of immunodeficiency in humans. Immunol. Rev., 2005, Vol. 203, pp. 143-155.
14. Wells P.R., Smogorzewska E., Barr M. Neonatal thymectomy: does it affect immune function? J. Thorac. Cardiovasc. Surg., 1998, Vol. 115, pp. 1041-1046.
15. Young J.L., Ramage J.M., Gaston J.S., Beverley P.C. In vitro responses of human CD45R0brightRA- and CD45R0-RAbright T cell subsets and their relationship to memory and naive T cells. Eur. J. Immunol. 1997, Vol. 27, pp. 2383-2390.
16. http://www.eurocat-network.eu (дата обращения: 03.09.2015).
Review
For citations:
Rovda Yu.I., Shmulevich S.A., Shabaldin A.V., Lukoyanycheva E.B. SUBPOPULATION PROFILES OF T HELPER CELLS EXPRESSING CD45RA AND CD31 MARKERS IN CHILDREN AFTER THYMECTOMY PERFORMED UPON SURGICAL TREATMENT OF CONGENITAL HEART DISEASE. Medical Immunology (Russia). 2016;18(2):119-128. (In Russ.) https://doi.org/10.15789/1563-0625-2016-2-119-128
Views:
1122
Email this article 