EXPRESSION OF рSTAT3 TRANSCRIPTION FACTOR IN PERIPHERAL BLOOD MONONUCLEAR CELLS AND ITS LEPTIN-INDUCED MODULATION IN BRONCHIAL ASTHMA
https://doi.org/10.15789/1563-0625-2014-6-551-558
Abstract
The aim of this study was to evaluate expression rates of phosphorylated STAT3 (рSTAT3) transcription factor in peripheral blood mononuclear cells (PBMC) and some features of its modulation by leptin in various clinical forma of bronchial asthma (BA).
Materials and methods. We evaluated 25 healthy persons and 62 BA patients. pSTAT3 expression in PBMC was measured by means of flow cytometry.
Results. A phenomenon of increased pSTAT3 expression was registered in PBMC from BA patients. A most sufficient elevation of pSTAT3 levels was revealed in allergic clinical variant of the disorder. Leptin was shown to increase pSTAT3 expression in PBMCs from healthy persons only. In allergic BA, such effect of leptin was not observed, whereas a trend for pSTAT increase was shown in non-allergic BA. In allergic BA, we have revealed a direct correlation between the STAT3-mediated leptin signaling index and bronchial patency parameters. Appropriate inverse correlation was registered in non-allergic BA.
Conclusion. We have revealed some characteristics of рSTAT3 expression in PBMCs and its modulation by leptin in various clinical forms of BA, thus providing a potential basis for development of targeted therapy aimed for transduction of different cytokine signals mediated by STAT3 transcription factor.
About the Authors
V. N. Mineev
First I. Pavlov State Medical University, St. Petersburg, Russian Federation
Russian Federation
Russian Federation
T. M. Lalaeva
First I. Pavlov State Medical University, St. Petersburg, Russian Federation
Russian Federation
Russian Federation
T. S. Vasilieva
First I. Pavlov State Medical University, St. Petersburg, Russian Federation
Russian Federation
Russian Federation
A. A. Kuzmina
First I. Pavlov State Medical University, St. Petersburg, Russian Federation
Russian Federation
Russian Federation
197089, Russian Federation, St. Petersburg, L. Tolstoy str., 6/8. Phone: 7 (921) 359-62-95
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Review
For citations:
Mineev V.N., Lalaeva T.M., Vasilieva T.S., Kuzmina A.A. EXPRESSION OF рSTAT3 TRANSCRIPTION FACTOR IN PERIPHERAL BLOOD MONONUCLEAR CELLS AND ITS LEPTIN-INDUCED MODULATION IN BRONCHIAL ASTHMA. Medical Immunology (Russia). 2014;16(6):551-558. (In Russ.) https://doi.org/10.15789/1563-0625-2014-6-551-558
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