NAD(P)-DEPENDENT DEHYDROGENASE ACTIVITY IN PERIPHERAL BLOOD LYMPHOCYTES OF INFANTS WITH ENLARGEMENT OF PHARYNGEAL TONSILS
https://doi.org/10.15789/1563-0625-2014-4-381-384
Abstract
We have observed and examined 57 children 1 to 3 years old diagnosed with enlargement of pharyngeal tonsils. A control group was presented by 35 healthy children. Bioluminescence technique was applied for studying NAD(P)-dependent dehydrogenase activity in peripheral blood lymphocytes. Activation of aerobic respiration and increasing activity of pentose phosphate cycle-dependent plastic processes were registered in blood lymphocytes of children with hypertrophic pharyngeal tonsils; along with decreased function of malate-aspartate shunt in energy metabolism of the cells, diminished anaerobic reaction of NADHdependent LDH, lower interaction between Krebs cycle and reactions of amino acid metabolism, and reduced activity of glutathione reductase.
About the Authors
L. M. Kurtasova
Krasnoyarsk V.F. Voyno-Yasenetsky State Medical University of the Ministry of Health Care of Russian Federation, Krasnoyarsk
Russian Federation
Russian Federation
PhD, MD (Medicine), Professor, Department of Clinical Immunology, Krasnoyarsk V.F. Voino-Yasenetsky State Medical University, Russian Ministry of Health Care
A. R. Shmidt
Krasnoyarsk Regional Centre of Preventive Care and Control of Aids, Krasnoyarsk
Russian Federation
Pediatrician, Krasnoyarsk Regional Centre of Preventive Care and Control of AIDS
Russian Federation
Pediatrician, Krasnoyarsk Regional Centre of Preventive Care and Control of AIDS
T. V. Lubnina
Krasnoyarsk Regional Centre of Preventive Care and Control of Aids, Krasnoyarsk
Russian Federation
Pediatrician, Krasnoyarsk Regional Centre of Preventive Care and Control of AIDS
Russian Federation
Pediatrician, Krasnoyarsk Regional Centre of Preventive Care and Control of AIDS
References
1. Савченко А.А., Сунцова Л.И. Высокочувствительное определение активности дегидрогеназ в лимфоцитах периферической крови биолюминесцентным методом // Лабораторное дело. 1989. № 11. С. 23-25. [Savchenko A.A., Suntsova L.I. Vysokochuvstvitel`noe opredelenie aktivnosti degidrogenaz v limfotsitakh perifericheskoy krovi biolyuminestsentnym metodom [Highly sensitive determination of dehydrogenase activity in peripheral blood lymphocytes by bioluminescent method]. Laboratornoe delo = Laboratory Science, 1989, no. 11, pp. 23-25.]
2. Терскова Н.В., Николаева А.И., Вахрушев С.Г., Смбатян А.С. Загрязнение атмосферного воздуха как фактор риска гипертрофии глоточной миндалины // Сибирское медицинское обозрение. 2013. № 5. С. 59-64. [Terskova N.V., Nikolaeva A.I., Vakhrushev S.G., Smbatyan A.S. Zagryaznenie atmosfernogo vozdukha kak faktor riska gipertrofii glotochnoy mindaliny [Air pollution as a risk factor of pharyngeal tonsils enlargement]. Sibirskoe meditsinskoe obozrenie = Siberian medical review, 2013, no. 5, рр. 59-64.]
3. Abbrescia D.I., La Piana G., Lofrumento N.E. Malate-aspartate shuttle and exogenous NADH / cytochrome electron transport pathway as two independent cytosolic reducing equivalent transfer systems. Arch. Biochem. Biophys., 2012, Vol. 518, no. 2, pp. 157-163.
4. Boym A. Isolation of lymphocytes from blood and bone marrow. Scand. J. Clin. Lab. Invest., 1968, Vol. 21, no. 97, pp. 77-80.
5. De la Roche M., Tessier S.N., Storey K.B. Structural and functional properties of glycerol-3-phosphate degydrogenase from a mammalian hibernator. Protein J., 2012, Vol. 31, no. 2, pp. 109-119.
6. Hsich S.Y., Chen S.H., Hung H.C. Functional roles of the tetramer organization of malic enzyme. J. Biol. Chem., 2009, no. 27, pp. 18096-18105.
7. Lu M., Banerjee S., Saidel G.M., Yu X. Regulation of cytosolic and mitochondrial oxidation via malate-aspartate shuttle: an observation using dynamic 13С NMR spectroscopy. Adv. Exp. Med. Biol., 2011, Vol. 701, pp. 185-192.
8. Norris M.G., Malys N. What is the true enzyme kinetics in the biological system? An investigation of macromolecular crowding effect upon enzyme kinetics of glucose-6-phosphate dehydrogenase. Biochem. Biophys. Res. Commun, 2011, Vol. 405, no. 3, pp. 388-392.
9. Pallardo F.V., Markovic J., Garcia-Gimenez J.L., Vina J. Role of nuclear glutathione as a key regulator of cell proliferation. Mol. Aspects Med., 2009, Vol. 30, no. 1, pp. 77-85.
10. Stanton R.C. Glucose-6-phosphate dehydrogenase, NADPH, and cell survival. IUBMB Life, 2012, Vol. 64, no. 5, pp. 362-369.
11. Tandogan B., Sengezer C., Ulusu N.N. In vitro effects of imatinib on glucose-6-phosphate degydrogenase and glutathione reductase. Folia Biol. (Praha), 2011, Vol. 57, no. 2, pp. 57-64.
Review
For citations:
Kurtasova L.M., Shmidt A.R., Lubnina T.V. NAD(P)-DEPENDENT DEHYDROGENASE ACTIVITY IN PERIPHERAL BLOOD LYMPHOCYTES OF INFANTS WITH ENLARGEMENT OF PHARYNGEAL TONSILS. Medical Immunology (Russia). 2014;16(4):381-384. (In Russ.) https://doi.org/10.15789/1563-0625-2014-4-381-384
Views:
1105
Email this article 